Microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme

A microfluidic chip and creatine kinase technology, applied in the field of protein detection, can solve the problems of multiple washing steps, low sensitivity, long incubation time, etc., and achieve the effect of strong reaction specificity, good stability and reliable results

Pending Publication Date: 2021-08-27
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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Problems solved by technology

ELISA kits can meet the requirements of portability and have been widely used in the screening of CKMB. However, this method has disadvantages such as long incubation time, many washing steps, and low sensitivity.

Method used

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  • Microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme
  • Microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme
  • Microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme

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Embodiment Construction

[0045] Preferred embodiments of the present invention will be described in more detail below. Although preferred embodiments of the present invention are described below, it should be understood that the present invention can be embodied in various forms and should not be limited by the embodiments set forth herein.

[0046] This example is used to illustrate the DNA hydrogel-based microfluidic chip detection method for creatine kinase isoenzyme detection in human serum, which specifically includes the following steps:

[0047] In the example, chloroauric acid (HAuCl 4 ·H 2 O) (>99.5%), sodium citrate, ammonium persulfate (APS), tetramethylethylenediamine (TEMED), and magnesium chloride were purchased from Damao Chemical Reagent Factory (Tianjin, China). Bovine serum albumin (BSA), 10,000×SYBRGreen (SG), 30% acrylamide (AA) were purchased from Solebol (Beijing, China). 10×DNA loading buffer, 20bp DNA ladder was purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China). ...

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Abstract

The invention belongs to the field of protein detection, and relates to a microfluidic chip colorimetric detection method and kit for detecting creatine kinase isoenzyme. The method comprises the following steps: S1, preparing a linear polyacrylamide-DNA polymer; S2, synthesizing and modifying gold nanoparticles; S3, designing and assembling a micro-fluidic chip; S4, synthesizing DNA hydrogel; and S5, detecting. According to the invention, the DNA hydrogel is used for detecting the target object, the stability is good, the reaction specificity is high, and visual detection can be achieved; and the micro-fluidic chip is used for detection, and the purpose of portable quantitative detection is achieved.

Description

technical field [0001] The invention belongs to the field of protein detection, and in particular relates to a microfluidic chip colorimetric detection method and a kit for detecting creatine kinase isoenzymes. Background technique [0002] Acute myocardial infarction (AMI) is a common cause of death worldwide. According to WHO statistics, among the 17.9 million deaths from cardiovascular diseases in 2015, about 8.9 million people died from ischemic heart disease, and acute myocardial infarction is the main cause of it, and it tends to be younger. Therefore, rapid and effective diagnosis of acute myocardial infarction has important clinical significance. Due to the suddenness and unpredictability of the onset of myocardial infarction, it is urgent to develop a portable detection method that can be quickly and effectively diagnosed on any occasion. [0003] Detecting the concentration of biomarkers in body fluid is an effective method for diagnosing acute myocardial infarct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/58G01N21/78
CPCG01N33/573G01N33/587G01N21/78G01N2333/9123G01N2800/324
Inventor 高志贤王瑜韩铁彭媛李双韩殿鹏任舒悦秦康陈梦梦
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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