39 results about "Creatine kinase isoenzymes" patented technology

The invention provides a test paper used for detecting acute myocardial infarction, and a preparation method and an application method thereof. The test paper comprises a base plate and sequentially closely connected components adhered to the base plate, wherein the components are a sample absorption pad, a bonding pad, a chromatography membrane, and a water absorption pad. The bonding pad is coated with a glucan-antibody-fluorescein mixed marker. The chromatography membrane is provided with a detection band and a control band. A monoclonal antibody is fixed on the detection band. A rabbit anti-mouse IgG antibody which can be speficically bound with the glucan-antibody-fluorescein mixed marker is fixed on the control band. The sensitivity range of the reagent provided by the invention reaches 0.1ng/L. With the test paper, quantitative detections upon trace myoglobin, troponin I and creatine kinase isoenzyme in myocardial infarction patient serum can be realized. A result can be obtained in 3-5 minutes after sample spotting. The operation is simple, and professional operation is not needed.

The invention particularly discloses a double-antibody sandwich immunoassay in-vitro diagnostic reagent suitable for screening progressive muscular dystrophy of newborns. The double-antibody sandwichimmunoassay in-vitro diagnostic reagent is used for specifically detecting creatine kinase isoenzyme CK-MM. According to the invention, a filter paper dry blood slide calibrator and a filter paper dryblood slide sample are adopted and are not influenced by AK released by red blood cells during hemolysis, so that the universality of screening the progressive muscular dystrophy of newborns is realized. The invention discloses a filter paper dry blood slide calibrator preparation method, which solves the instability of the calibrator and prevent the hydrolysis of creatine kinase isoenzyme and/orsubtype by carboxyl peptidase in blood plasma. The invention discloses a special filter paper dry blood slide sample eluent, which solves the stability problem of a to-be-detected object during sample detection and reduces the interference of external factors. After muscular dystrophy is diagnosed in the early stage and proper treatment measures are taken, the progress of the disease can be greatly delayed, the life quality of a patient is improved, and huge cost caused by blind medical treatment of the patient can also be avoided.

The invention relates to a novel separated binding protein comprising a CKMB (Hybrid Creatine Kinase Isoenzymes) antigen binding structural domain, and researches the aspects of preparation, application and the like of the binding protein. The binding protein has high activity, has a very high affinity with a human CKMB protein, and can be widely applied to the field of detection of the CKMB protein.

The invention belongs to the field of protein detection, and relates to a rolling circle amplification-gold tetrahedron colorimetric detection method and kit for detecting creatine kinase isoenzyme. The detection method comprises the following steps: S1, preparing a magnetic bead-aptamer-complementary chain compound; S2, preparing a DNA-AuNPs compound; S3, preparing an AuNPs tetrahedron; S4, preparing a rolling circle amplification template; S5, carrying out competitive reaction on creatine kinase isoenzyme and a complementary chain; S6, performing rolling circle amplification; S7, carrying out aggregation chromogenic reaction on the gold tetrahedron; and S8, conducting reading. According to the invention, an isothermal amplification technology is utilized, a complicated temperature change process is not needed, stability is relatively good, and sensitivity is relatively high. Compared with other detection methods, the colorimetric method disclosed by the invention does not need a large instrument for detection, can obtain a detection result through imaginJ software, is relatively low in cost, has a detection limit of 0.05 pM, and is suitable for on-site screening and rapid detection processes.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a creatine kinase isoenzyme CK-MB detection kit and a detection method thereof. The creatine kinase isoenzyme CK-MB detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises chromium nicotinate, an imidazole buffer solution, diadenosine pentaphosphate (AP5A), adenosine monophosphate (AMP), glucose-6-phosphate dehydrogenase, magnesium acetate, glucose, nicotinamide adenine dinucleotide phosphate (NADP <+>) and an anti-CK-MB antibody; the R2 reagent comprises an imidazole buffer solution, adenosine diphosphate (ADP), phosphocreatine, sodium azide and glucokinase. Meanwhile, the invention further provides a method for detecting the content or concentration of the creatine kinase isoenzyme CK-MB, the method adopts the creatine kinase isoenzyme CK-MB detection kit to detect the content or concentration of the creatine kinase isoenzyme CK-MB, and the detection accuracy can be effectively improved.

The invention provides a high-sensitivity creatine kinase isoenzyme detection kit and a preparation method thereof. The high-sensitivity creatine kinase isoenzyme detection kit comprises a reagent R1and a reagent R2; the preparation method comprises the steps: reacting an anti-mouse IgG-Fc antibody with amino groups on the surfaces of latex microspheres to form a shift alkali, directionally marking the latex microspheres with the anti-mouse IgG-Fc antibody, connecting the anti-mouse IgG-Fc antibody with at least two mouse anti-human CK-MB monoclonal antibodies, and coupling the latex microspheres with the mouse anti-human CK-MB monoclonal antibodies to prepare a latex microsphere reagent; and adding the latex microsphere reagent into a closed buffer solution, carrying out resuspension ultrasonic treatment, closing, adding a latex preservation solution, carrying out resuspension ultrasonic treatment to prepare latex reagents, and mixing at least two latex reagents to prepare a reagentR2. Compared with the prior art, the kit is high in sensitivity, wide in detection range, good in repeatability, high in specificity and good in stability; compared with other coupling methods, the method provided by the invention has the advantages that the CK-MB monoclonal antibody F (ab) end active sites are prevented from being coupled to the microspheres, meanwhile, the CK-MB monoclonal antibody coupling amount is increased, and the reagent detection sensitivity is obviously improved.

The invention relates to the technical field of immunology, and particularly discloses an anti-creatine kinase isoenzyme antibody and a preparation method, application and amino acid sequence thereof.The anti-creatine kinase isoenzyme antibody comprises an amino acid sequence of a variable region of a heavy chain, wherein the amino acid sequence comprises a sequence shown in SEQ ID NO: 1; and anamino acid sequence of a variable region of a light chain, wherein the amino acid sequence comprises a sequence shown in SEQ ID NO: 2. The sequence shown as SEQ ID NO: 1 and the sequence shown as SEQID NO: 2 have at least one substitution. The antibody has the characteristics of high specificity and strong affinity.

The invention provides a latex enhanced immunoturbidimetry kit for detecting creatine kinase isoenzyme CK-MB. The latex enhanced immunoturbidimetry kit comprises a reagent R1 and a reagent R2, whereinthe reagent R1 comprises a buffer solution, a coagulant, a blocking agent, a surfactant, a protective agent and a preservative, and the reagent R2 comprises a buffer solution, CK-MB antibody coated nano microspheres, a protective agent and a preservative. A rabbit anti-mouse IgM antibody or a SeaBlock fish plasma blocking agent is adopted as the blocking agent in the reagent 1, and glycine, ethanolamine and calf serum are adopted as a sealing agent in the CK-MB antibody coated nano microspheres of the reagent 2, so that the detection interference can be effectively reduced. The kit provided by the invention has the advantages of strong anti-interference performance, high sensitivity, wide detection range, good repeatability, high specificity, good stability and the like, can realize automatic detection on a biochemical analyzer, replace a chemiluminescence product and reduce the detection cost, and meets clinical use.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a CK-MB type creatine kinase isozyme and a preparation method and application thereof. The CK-MB type creatine kinase isozyme obtained by the preparation method provided by the invention is relatively high in yield, and the yield of M subunit protein and B subunit protein after equal-proportion renaturation is 35mg/L. The CK-MB type creatine kinase isoenzyme obtained by the preparation method disclosed by the invention performs quantitative detection by using a Roche creatine kinase isoenzyme detection kit (an electrochemical luminescence method), and the activity rate of the CK-MB type creatine kinase isoenzyme is greater than 41%. The preparation method of the CK-MB type creatine kinase isoenzyme provided by the invention is simple to operate, low in cost and high in yield. The prepared CK-MB type creatine kinase isoenzyme is high in biological activity and good in stability, can be used as a quality control product or a calibration product in in-vitro diagnosis, and can also be used as immunogen or screening antigen for creatine kinase isoenzyme antibody development.

The invention discloses a phosphocreatine kinase isoenzyme detection kit, and relates to the technical field of biomedicine. The kit includes a first reagent, wherein the first reagent comprises the following components: imidazole with concentration of 124 to 126mmol/L; magnesium acetate with concentration of 2 to 3mmol/L; ethylenediamine tetraacetic acid with concentration of 12-13 mmol/L; mannitol with concentration of 34 to 34.3 mmol/L; adenosine monophosphate with concentration of 6 to 6.5 mmol/L; lithium diadenosine pentaphosphate with concentration of 0.01 to 0.015 mmol/L, reductive coenzyme II with concentration of 2 to 3mmol/L, Proclin150 with concentration of 0.8 to 1.2 g/L, hexokinase with concentration of 3.5 to 4ku/L, N-acetylcysteine with concentration of 24 to 26mmol/L, sodium sulfite with concentration of 2 to 3mmol/L, and anti-human CK-M antibody with concentration of 2 to 3 ml/L. The improved phosphocreatine kinase isoenzyme detection kit provided by the invention is higher in sensitivity, better in repeatability, higher in accuracy and better in stability.

The invention discloses a sandwich immunoassay kit. The invention provides an application of a surface-enhanced Raman detection kit in creatine kinase isoenzyme detection, the kit comprises a top layer reagent, a bottom layer reagent, a BSA solution and an EDTA anticoagulant, the top layer reagent in the kit adopts 3-mercapto-1, 2, 4-triazole molecules as Raman detection molecules, and compared with common P-ATP molecules in the prior art, the 3-mercapto-1, 2, 4-triazole molecules have a stronger Raman signal enhancing function; the surface-enhanced Raman detection kit is applied to the detection of creatine kinase isoenzyme in a human body for the first time, can make up for the defects in the existing detection technology, greatly improves the detection limit and sensitivity, and plays a key role in preventing sepsis.

The invention discloses a high-sensitivity creatine kinase isoenzyme quantitative detection test strip which comprises a connecting plate and a reagent tube integrally formed with the connecting plate, the connecting plate is sequentially divided into a handheld area, a reagent area, a washing area and a detection area, antiskid lines are arranged on an upper surface and a lower surface of the handheld area, and a sample reagent tube and a main reagent tube are arranged in the reagent area; a streptavidin magnetic particle solution, a biotin-labeled creatine kinase isoenzyme antibody solution,an acridinium ester-labeled creatine kinase isoenzyme antibody solution, an acidic excitation solution and an alkaline excitation solution are respectively arranged in the main reagent tube; the principle of a double-antibody sandwich method is adopted, meanwhile, an avidin-biotin system is utilized, acridinium ester chemiluminiscence is adopted, the detection sensitivity of the test strip is greatly improved, the reaction time is shortened, and the reagent cost is reduced.