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Creatine kinase isoenzyme detection kit

A technology for detecting kits and creatine kinase, which is applied in measuring devices, instruments, and biological material analysis, etc., can solve the problems such as the kit detection linear range is not wide, the reagent storage is unstable, and the anti-interference ability is poor, so as to avoid defects Influence, improve the detection range, avoid the effect of interference

Pending Publication Date: 2022-05-13
广西康柏莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are creatine kinase isoenzyme detection kits based on latex-enhanced immunoturbidimetric assay on the market, these kits have problems such as not wide detection linear range, low sensitivity, poor anti-interference ability, and unstable storage of reagents.

Method used

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  • Creatine kinase isoenzyme detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The creatine kinase isoenzyme detection kit provided in this embodiment includes reagent R1 and reagent R2, and the composition of the reagent R1 and reagent R2 is as follows:

[0044] Reagent R1:

[0045]

[0046]

[0047] In the table: the mass ratio of TritonX-100 and styrene polyoxyethylene ether is 3.2:1.

[0048] Preparation of reagent R1: according to the components and content of reagent R1 in the above table, take the components of reagent R1, add them into purified water, mix well, adjust the pH value to 7.0, filter through a filter membrane, and constant volume to obtain reagent R1 .

[0049] Reagent R2:

[0050]

[0051] In the table: the mass ratio of triethanolamine, glycine and casein is 0.5:1:2.5.

[0052] Preparation of reagent R2:

[0053] ①Microsphere activation: Add polystyrene latex microspheres with a diameter of 300nm and surface-modified carboxyl groups to the MOPS buffer with a pH of 7.2, and add 1-ethyl-(3-dimethylaminopropyl) carb...

Embodiment 2

[0059] The creatine kinase isoenzyme detection kit provided in this embodiment includes reagent R1 and reagent R2, and the composition of the reagent R1 and reagent R2 is as follows:

[0060] Reagent R1:

[0061]

[0062] In the table: the mass ratio of TritonX-100 and styrene polyoxyethylene ether is 2.5:1.

[0063] Preparation of reagent R1: According to the components and contents of reagent R1 in the above table, take each component of reagent R1, add them into purified water, mix well, adjust the pH value to 6.5, filter through a filter membrane, and constant volume to obtain reagent R1 .

[0064] Reagent R2:

[0065]

[0066] In the table: the mass ratio of triethanolamine, glycine and casein is 1:1:3.

[0067] Preparation of reagent R2:

[0068] ①Microsphere activation: Add polystyrene latex microspheres with a diameter of 400nm and surface-modified amino groups to the HEPES buffer with a pH of 6.0, and add mercaptonicotinamide, react at 30°C for 35min, and re...

Embodiment 3

[0074] The creatine kinase isoenzyme detection kit provided in this embodiment includes reagent R1 and reagent R2, and the composition of the reagent R1 and reagent R2 is as follows:

[0075] Reagent R1:

[0076]

[0077] In the table: the mass ratio of TritonX-100 and styrene polyoxyethylene ether is 4.5:1.

[0078] Preparation of reagent R1: According to the components and content of reagent R1 in the above table, take each component of reagent R1, add them into purified water, mix well, adjust the pH value to 8.0, filter through a filter membrane, and constant volume to obtain reagent R1 .

[0079] Reagent R2:

[0080]

[0081]

[0082] In the table: the mass ratio of triethanolamine, glycine and casein is 2:1:4.

[0083] Preparation of reagent R2:

[0084] ①Microsphere activation: Add polystyrene latex microspheres with a diameter of 250nm and surface-modified hydroxyl groups to the MOPSO buffer with a pH of 7.5, and add isocyanate, react at 25°C for 40min, re...

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Abstract

The invention discloses a creatine kinase isoenzyme detection kit, and belongs to the technical field of biological detection. The creatine kinase isoenzyme detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffer solution, a coagulant, a chelating agent, a blocking agent, a surfactant, a stabilizer and a preservative; and the reagent R2 comprises the following components: a buffer solution, latex particles coated with creatine kinase isoenzyme antibodies, a sealing agent, a stabilizer and a preservative. The creatine kinase isoenzyme detection kit disclosed by the invention overcomes the defects of the existing creatine kinase isoenzyme detection kit, and has the advantages of strong anti-interference performance, high sensitivity, high specificity, high accuracy, wide linear range, good stability and the like; and a safe, rapid, accurate and pollution-free detection means is provided for the detection of the creatine kinase isoenzyme.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a creatine kinase isozyme detection kit. Background technique [0002] Creatine kinase (CK) is a dimeric enzyme that has four different forms: mitochondrial isozyme, cytoplasmic isozyme CK-MM (muscle type), creatine kinase brain isozyme CK- BB (brain type), creatine kinase isoenzyme (CK-MB), in which creatine kinase isoenzyme is mainly located in the myocardium. [0003] Creatine kinase isoenzyme has high sensitivity and specificity in diagnosing transmural myocardial infarction, its expression level is low under normal conditions, creatine kinase isoenzyme is excreted from damaged cells 4-8 hours after onset And rapidly begins to increase in the blood, reaches the highest level after 12-24 hours, and returns to normal within 24-48 hours. If the level of creatine kinase isoenzyme remains high after acute myocardial infarction, it indicates that myocardial necrosis ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/573G01N33/546G01N33/543
CPCG01N33/577G01N33/573G01N33/54313G01N2333/9123
Inventor 李名星张设熙莫智林韦佳志韦松利苏琴
Owner 广西康柏莱科技有限公司
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