Test paper used for detecting acute myocardial infarction, and preparation method and application method thereof

A technology for acute myocardial infarction and test strips, which is applied in the field of test strips for detecting acute myocardial infarction, can solve the problems of impossible and quantitative detection of trace amounts, and achieves the effect of less sample consumption and improved sensitivity

Inactive Publication Date: 2013-01-30
武汉渊元医学科技有限公司
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In contrast, the sensitivity of colloidal gold technology is worse than that of ELISA, which can only reach 50ng/ml. It is impossible. In add

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  • Test paper used for detecting acute myocardial infarction, and preparation method and application method thereof
  • Test paper used for detecting acute myocardial infarction, and preparation method and application method thereof
  • Test paper used for detecting acute myocardial infarction, and preparation method and application method thereof

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[0025] The technical solutions of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0026] The principle of the present invention is that the detection method involved in the present invention is a double-antibody sandwich method, one is a coated antibody, and the other is a labeled antibody to detect the antigen in the serum. The conventional antibody label uses horseradish peroxidase to label the antibody. However, horseradish peroxidase labeling has a problem of poor sensitivity. Fluorescein-labeled antibody technology can greatly improve the sensitivity of detection reagents. As f...

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Abstract

The invention provides a test paper used for detecting acute myocardial infarction, and a preparation method and an application method thereof. The test paper comprises a base plate and sequentially closely connected components adhered to the base plate, wherein the components are a sample absorption pad, a bonding pad, a chromatography membrane, and a water absorption pad. The bonding pad is coated with a glucan-antibody-fluorescein mixed marker. The chromatography membrane is provided with a detection band and a control band. A monoclonal antibody is fixed on the detection band. A rabbit anti-mouse IgG antibody which can be speficically bound with the glucan-antibody-fluorescein mixed marker is fixed on the control band. The sensitivity range of the reagent provided by the invention reaches 0.1ng/L. With the test paper, quantitative detections upon trace myoglobin, troponin I and creatine kinase isoenzyme in myocardial infarction patient serum can be realized. A result can be obtained in 3-5 minutes after sample spotting. The operation is simple, and professional operation is not needed.

Description

technical field [0001] The invention relates to the field of immunodiagnostic reagents, in particular to a test paper for detecting acute myocardial infarction, and a method for preparing and applying the test paper. Background technique [0002] With the aggravation of the aging process of our country's population, acute myocardial infarction has become a major disease that endangers China. The World Health Organization pointed out that 8.5 million people die of acute myocardial infarction every year in the world. In my country, there are 20 million patients with acute myocardial infarction every year, and 1 million people are killed by acute myocardial infarction. Therefore, acute myocardial infarction has become a major disease that endangers the health of Chinese people. Treatment shows that within 3 hours after the onset of acute myocardial infarction is a critical time for treatment. Only by early prevention, early detection, and early treatment of myocardial infarc...

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/573G01N33/558G01N33/533
Inventor 江建华
Owner 武汉渊元医学科技有限公司
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