Creatine kinase isoenzyme CK-MB detection kit and detection method thereof

Abstract

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a creatine kinase isoenzyme CK-MB detection kit and a detection method thereof. The creatine kinase isoenzyme CK-MB detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises chromium nicotinate, an imidazole buffer solution, diadenosine pentaphosphate (AP5A), adenosine monophosphate (AMP), glucose-6-phosphate dehydrogenase, magnesium acetate, glucose, nicotinamide adenine dinucleotide phosphate (NADP <+>) and an anti-CK-MB antibody; the R2 reagent comprises an imidazole buffer solution, adenosine diphosphate (ADP), phosphocreatine, sodium azide and glucokinase. Meanwhile, the invention further provides a method for detecting the content or concentration of the creatine kinase isoenzyme CK-MB, the method adopts the creatine kinase isoenzyme CK-MB detection kit to detect the content or concentration of the creatine kinase isoenzyme CK-MB, and the detection accuracy can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a creatine kinase isoenzyme CK-MB detection kit and a detection method thereof. Background technique [0002] The creatine kinase isoenzyme CK-MB is mainly distributed in the myocardium. After the onset of acute myocardial infarction with chest pain, the serum CK-MB level increases. Serum CK-MB is an important indicator for diagnosing acute myocardial infarction and determining whether there is myocardial necrosis. Especially for acute myocardial infarction and recurrent myocardial infarction without Q wave in the electrocardiogram, the increase of serum CK-MB is decisive. The role of diagnosis. [0003] The creatine kinase isoenzyme (CKMB) assay kit is a relatively mature routine item on the market and registered by most IVD manufacturers. The existing creatine kinase isozyme (CK-MB) assay kit has the following defects: creatine kinase CK has three isozyme...

AI Technical Summary

Problems solved by technology

How does hemolysis lead to high creatine kinase isoenzyme CK-MB? The reason is that red blood cells contain a very small amount of creatine kinase isoenzyme CK-MB and a large amount of adenylate kinase AK. Adenylate kinase AK will be released into the serum. Adenylate kinase AK is an enzyme for the interconversion between adenosine diphosphate ADP and adenosine triphosphate ATP. The glycoside ADP can be converted into adenosine triphosphate ATP under the catalysis of adenylate kinase AK, and adenosine triphosphate ATP will react with the reagent to generate a certain product, which can interfere with the colorimetric reaction, resulting in an increase in the concentration of the reaction product, and finally causing all Pseudo-increased creatine kinase isoenzyme CK-MB measured

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