60 results about "Nicotinuric acid" patented technology

This invention discloses a method for efficient genetic transformation of rice that overcomes genotype dependence and a dedicated culture medium, relating to the field of biotechnology. The dedicated culture medium comprises KCl, KH₂PO₄, MgSO₄·7H₂O, CaCl₂·2H₂O, disodium ethylenediaminetetraacetate, FeSO₄·7H₂O, MnSO₄·H₂O, ZnSO₄·7H₂O, H₃BO₃, KI, CoCl₂·6H₂O, CuSO₄·5H₂O, Na₂MoO₄·2H₂O, glutamine, aspartic acid, arginine, glycine, cyclohexanehexyl alcohol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, and a carbon source. Using this dedicated culture medium can increase the number of rice cells containing the NiR gene. 375 S 453 The genetic transformation efficiency of haplotype rice varieties is of great significance for overcoming the bottleneck of rice genotype dependence, promoting functional genomics research and molecular breeding.

The invention discloses a phagostimulant capable of improving the prevention and control effect of cotton flower thrips and application of the phagostimulant. The phagostimulant is prepared from the following components in parts by weight: 10 to 30 parts of p-methoxycinnamyl aldehyde, 5 to 40 parts of anisaldehyde, 40 to 70 parts of ethyl nicotinate and 10 parts of a cosolvent. The phagostimulant is prepared from the following components in parts by weight: 10 to 30 parts of p-methoxycinnamyl aldehyde, 5 to 40 parts of anisaldehyde, 40 to 70 parts of ethyl nicotinate and 10 parts of cosolvent. By utilizing the tropism of the cotton flower thrips to specific volatile compounds and through the synergistic effect of the three plant source compounds, pests hidden in stamens, petal wrinkles, leaf backs and other hidden parts are attracted to a pesticide application area, the contact area of insect bodies and the pesticide is remarkably increased, and therefore the prevention and control effect is improved. Field tests show that when an optimal formula (the ratio of p-methoxycinnamaldehyde to anisaldehyde to ethyl nicotinate to the cosolvent is 20: 20: 50: 10) is matched with spinetoram for use, the control effect can reach 84.43%-92.22%, the control effect is remarkably improved compared with that of single use of an insecticide, and a good control effect is shown. The method is safe and environment-friendly, reduces the amount and increases the efficiency, and has remarkable economic and ecological benefits.

This invention discloses a highly efficient catalytic synthesis process for nicotinamide intermediates using the field of organic synthesis technology. The process includes: adding appropriate amounts of dichloronicotinic acid, organic solvent, lanthanum-doped composite metal fluoride-diatomaceous earth supported catalyst, and encapsulated 4A molecular sieve to a reaction vessel under nitrogen protection; purging with nitrogen, heating, and stirring; introducing anhydrous dimethylamine gas, controlling the pressure, and continuing stirring; after the reaction is complete, washing with sodium bicarbonate aqueous solution, washing with deionized water, drying, concentrating, and recrystallizing to obtain the target product. The lanthanum-doped composite metal fluoride-diatomaceous earth supported catalyst prepared by this invention is recyclable, has mild reaction conditions, high product yield, is environmentally friendly, and suitable for industrial production.

This invention provides an induction culture medium and method for using marigold anthers as explants. The induction culture medium is based on MS solid medium containing agar, with specific amounts of sucrose, tryptophan, phenylalanine, arginine, casein amino acids, anhydrous asparagine, 6-benzylpurine, naphthaleneacetic acid, silver nitrate, pyridoxine hydrochloride, thiamine hydrochloride, nicotinic acid, D-biotin, folic acid, ascorbic acid, and coconut juice added. This invention also provides a method for induction culture using this induction culture medium. This invention can greatly improve the callus induction rate of marigolds, significantly increasing the number of regenerated seedlings, and provides technical guidance for the cultivation of haploid marigolds using anthers.

The present invention belongs to the technical field of biomedicine, and relates to a class of dexborneol or fenchyl alcohol ester derivatives of 2-hydroxynicotinic acid, and pharmaceutical uses thereof. The structural general formula of the derivatives is (1), R1 being selected from -H, -Cl, a hydrocarbon group having 1-8 carbon atoms, -CF3, -CF2H, -CN, -SCH3, -OH, -CHO, -CH2OH, -CH2NH2, -COOH, halogenated phenyl, or -NR4R5; R4, R5 are independently selected from -H or -CH3; R2 is selected from -H, an alkyl group having 1-6 carbon atoms, an acyl group having 2-7 carbon atoms, or a carbamoyl group; R 3 is selected from formula (a) or formula (b); X is selected from -CH= or -N=. The derivatives have good inflammation-inhibiting effects, and at high concentrations, they exhibit low cytotoxicity and are safer. The derivatives can be used for preparing a drug for treating inflammation and inflammation-related diseases such as stroke, neuropathic pain, depression and Alzheimer's disease.

The present disclosure provides antibodies and polypeptides that specifically bind to nicotinic acid phosphoribosyltransferase (NAPRT). Also provided are nucleic acids encoding the antibodies, expression vectors and host cells for making the antibodies, methods of using the antibodies to detect NAPRT in a sample from a subject in need thereof, methods of using the antibodies to diagnose a subject having cancer or sepsis. Compositions comprising these antibodies are also provided. Also provided are compositions comprising a nicotinamide phosphoribosyltransferase inhibitor (NAMPTi), and methods of using the NAMPTi to treat cancer following diagnosis with the NAPRT antibody.

The invention discloses an efficient rice genetic transformation method capable of overcoming genotype dependence and a special culture medium, and relates to the technical field of biology. The special culture medium is prepared from KCl, KH2PO4, MgSO4. 7H2O, CaCl2. 2H2O, disodium ethylene diamine tetraacetate, FeSO4. 7H2O, MnSO4. H2O, ZnSO4. 7H2O, H3BO3, KI, CoCl2. 6H2O, CuSO4. 5H2O, Na2MoO4. 2H2O, glutamine, aspartic acid, arginine, glycine, inositol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid and a carbon source. By adopting the special culture medium, the genetic transformation efficiency of a rice variety containing the NiR gene E375S453 haplotype can be improved, and the special culture medium has important significance in overcoming the bottleneck of rice genotype dependence and promoting functional genome research and molecular breeding.

The application discloses a preparation method of a meta-aminobenzoic acid bisamide compound. The method is prepared by condensation reaction with N-(2-bromo-4-(perfluoropropane-2-yl)-6-(trifluoromethyl)phenyl)-2-fluoro-3-(hydroxyl amido)benzamide and 2-fluoropyridine-5-formyl chloride as raw materials. The 2-fluoropyridine-5-formyl chloride is prepared from 6-fluoronicotinic acid and phosgene. The preparation method takes the 2-fluoropyridine-5-formyl chloride with high yield and high purity as raw material, can omit the purification step, shorten the synthesis route, improve the preparation efficiency, improve the selectivity of the reaction, reduce the generation of impurities, improve the yield of the product, efficiently prepare the product with high yield and high purity, has the advantages of simple process, convenient operation, low cost, mild reaction condition, high preparation efficiency, high yield, high purity, environmental friendliness and the like, is suitable for large-scale preparation, and is convenient for industrial application.

The invention relates to the field of pesticide synthesis, in particular to a synthesis method of ethonicotinic acid. 5-ethyl pyridine-2. 3-dicarboxylic acid diethyl ester and 2-amino-2. 3-dimethyl butyramide are used as raw materials and react in a sodium ethoxide ethanol solution at the temperature of 50-70 DEG C, hydrogen chloride gas is introduced into a system for acidification after the reaction is completed, and imazethapyr and a by-product sodium chloride are obtained. The hydrogen chloride gas is directly introduced in the acidification process, and the acidification process is free of hydration, so that on one hand, no direct wastewater is generated, the generation of wastewater with high salt content and high organic matter content is avoided, the treatment cost is reduced, on the other hand, the byproduct sodium chloride is firstly removed from the system by controlling the ethanol amount of the system, and then concentration and crystallization are performed; the high-purity imazethapyr of which the purity is greater than 99% is obtained by utilizing the solubility of the solvent ethanol to impurities, and the quality of both byproducts and main products is at a higher level in the industry.

The present invention provides a preparation method of tozasertib, which relates to the technical field of medicine. The preparation method provided by the present invention comprises the following steps: (1) reacting methyl 2-cyanoisonicotinate with hydrazine hydrate, and crystallizing to obtain intermediate TPS-1; (2) reacting 4-cyanopyridine with a base, and then reacting completely with TPS-1, followed by filtration, washing and drying to obtain TPS-2a; (3) subjecting TPS-2a to a cyclization reaction with a reaction solvent, and after the reaction is complete, mixing with a crystallization solvent to obtain the product TPS. The product obtained by this process has a relatively high yield, with the highest total yield being about 62%, high purity, with a purity greater than 99.9%; the preparation method is simple, under the conditions of this preparation method, the raw materials react quickly, and at the same time, time-consuming operations such as extraction and concentration are avoided, and the product can be directly obtained by filtration after the reaction, with a short production cycle; the reaction conditions are easy to realize industrial operation.

To provide formulation technology with which it is possible to suppress browning that occurs over time in a topical composition containing a non-steroidal anti-inflammatory, 0.015 wt.% or more of vanillylamide nonanoate, and 0.01 wt.% or more of benzyl nicotinate.SOLUTION: In this topical drug composition, the browning that occurs over time can be suppressed effectively by blending a monoterpene with a non-steroidal anti-inflammatory, 0.015 wt.% or more of vanillylamide nonanoate, and 0.01 wt.% or more of benzyl nicotinate.SELECTED DRAWING: None

The application discloses a composition for improving anti-aging and firming effects and a preparation method thereof, and belongs to the technical field of cosmetics. The composition for improving anti-aging and firming effects comprises the following raw materials in parts by mass: galactomyces ferment l filtrate 20-60 parts, nicotinamide adenine dinucleotide 0.1-5 parts, myristyl nicotinate 0.5-5 parts, chrysanthemum coronarium leaf extract 0.2-4.5 parts, lens culinaris seed extract 0.1-5.5 parts and chrysanthemum coronarium extract 0.3-5 parts. The composition for improving anti-aging and firming effects has significant firming and anti-aging effects and is overall mild and non-irritating.

The application provides a preparation method of a medical intermediate 4-bromo-6-chloronicotinic acid methyl ester. The method takes 4,6-dichloronicotinic acid methyl ester as a starting material, reacts with an alkali salt to obtain 6-chloro-4-hydroxynicotinic acid methyl ester; then the 6-chloro-4-hydroxynicotinic acid methyl ester reacts with a bromination reagent under the action of an acid binding agent to obtain the target product 4-bromo-6-chloronicotinic acid methyl ester. The preparation method has short steps, the raw material is easy to obtain and low in price, the reaction conditions are mild, easy to purify, and simple to operate; meanwhile, the product has high yield, high purity, low production cost, is convenient for industrialized production, has a good application prospect, and plays a positive role in the research and development of downstream products of 4-bromo-6-chloronicotinic acid methyl ester.

This invention discloses a 2-hydroxynicotinic acid-2-hydroxybenzyl ester, its preparation method, and its application. The structural formula of the 2-hydroxynicotinic acid-2-hydroxybenzyl ester is shown below. This invention utilizes 2-hydroxynicotinic acid as a raw material and obtains a 2-hydroxynicotinic acid-2-hydroxybenzyl ester with a novel structure through esterification. The 2-hydroxynicotinic acid-2-hydroxybenzyl ester has low cytotoxicity, can scavenge reactive oxygen species, and has good anti-UVB-induced oxidative damage activity in HaCaT cells. It can be used in products for anti-skin oxidative damage.

The present application relates to the technical field of oral liquid, in particular to a kind of oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow and preparation method thereof, it is characterized in that, the oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow is made of the following components, by weight parts: artemisia capillaris 200~300 parts, gardenia 150~200 parts, bupleurum 100~150 parts, polygonatum 50~100 parts, radix paeoniae alba 50~100 parts, liquorice 10~50 parts, nicotinic acid 1~10 parts, propylene glycol 200~300 parts, fructose diphosphate sodium 1~10 parts, pure water 200~400 parts.The oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow can prevent and treat ketosis, fatty liver and liver damage at the same time, comprehensively improve the constitution of new cow, improve immunity, improve physical indicators and reproductive performance, increase economic benefits.

Disclosed in the present invention are a salt of a nicotinate compound, a crystal of the salt, and a preparation method therefor and a use thereof. The salt is selected from benzenesulfonate, phosphate, sulfate, hydrochloride, and methanesulfonate of a compound represented by formula (I). The crystal is crystal form A of the benzenesulfonate of the compound represented by formula (I), crystal form B of the benzenesulfonate of the compound represented by formula (I), crystal form A of the phosphate of the compound represented by formula (I), crystal form A of the sulfate of the compound represented by formula (I), crystal form A of the hydrochloride of the compound represented by formula (I), crystal form A of the methanesulfonate of the compound represented by formula (I), crystal form B of the methanesulfonate of the compound represented by formula (I), or an amorphous form of the methanesulfonate of the compound represented by formula (I).

The invention discloses a preparation method of 4-bromo-6-chloronicotinic acid, which is characterized by comprising the following steps: dissolving 2-chloro-4-amino-5-methylpyridine in dilute acid, then adding a catalyst and a bromide, and dropwise adding a sodium nitrite aqueous solution to diazotize the bromine to obtain 2-chloro-4-bromo-5-methylpyridine, wherein the molar ratio of the 2-chloro-4-amino-5-methylpyridine, the catalyst, the bromide and the sodium nitrite is 1:(1-3):(2-5):(2-5); and (2) reacting the 2-chloro-4-bromo-5-methylpyridine with an oxidizing agent to obtain 4-bromo-6-chloronicotinic acid, wherein the molar ratio of the 2-chloro-4-bromo-5-methylpyridine to the oxidizing agent is 1:(1-3). The method has the advantages of simple process circuit, mild and safe reaction conditions, low production cost and high total yield.

The invention relates to metabonomic characterization of microorganisms, in particular to a method for identifying cell types of cells in a sample, comprising: culturing the sample in a growth medium comprising nicotinamide to obtain a cultured growth medium; analyzing the cultured growth medium by chemical analysis; and identifying the cell type as at least one of the genus Escherichia, the genus Klebsiella, the genus Pseudomonas, the genus Enterococcus, the genus Staphylococcus or the genus Streptococcus when the cultured growth medium contains a higher concentration of nicotinate than the growth medium; wherein the growth culture medium comprises the following components in a buffer culture medium: 0.02 to 2 g / l of D-glucose, 0.0001 to 0.1 g / l of nicotinamide, 0.0001 to 0.1 g / l of pyridoxin.HCl, 0.02 to 1 g / l of hypoxanthine, 0.2 to 5 g / l of L-arginine, 0.2 to 5 g / l of L-threonine, 0.1 to 5 g / l of L-histidine, 0.02 to 2 g / l of spermine and catalase. According to the method, identification and toxin sensitivity detection can be completed within about half of time compared with that of an existing method.

The subject of the application is a tool for applying methyl nicotinate solution, which is characterized in that it consists of a base (1) in the shape of a cuboid. The lower surface of the base (1) has a T-shaped recess along its entire length, in which at least two T-shaped mounting brackets (2) are press-fitted. Square frames (3) are attached to the lower surfaces of the mounting brackets (2). Sponges (4) are press-fitted into the frames (3). A digital timer (5) is attached to the upper surface of the base (1) by gluing.

The present invention aims to provide an oral composition that has an astringent sensation while effectively suppressing discoloration over time. [Solution] An oral composition comprising (A) component: pyridoxine or a salt thereof, (B) component: fluorine-containing compound, and (C) component: an anionic surfactant, wherein the content of component (B) is such that when converted to a fluoride ion concentration it is 1000 to 4000 ppm, and the content of component (C) is such that it is 0.05 to 5% by mass, preferably further comprising (D) component: at least one selected from the group consisting of saccharin sodium, sucralose, xylitol, erythritol, maltitol, acesulfame potassium, trehalose, and mannitol, and more preferably further comprising (E) component: at least one selected from the group consisting of tocopherol acetate and tocopherol nicotinate.

The invention discloses a method for detecting beta-nicotinamide nucleotide (NMN), beta-nicotinamide adenine dinucleotide (NAD), nicotinic acid (NA) and nicotinamide (NAM) in cosmetics. The method comprises the following steps: (1) preparing a standard sample; (2) drawing a standard curve; (3) preparing a sample to be detected; (4) extracting the to-be-detected sample prepared in the step (3) with water, and (5) filtering an extracting solution obtained in the step (4), then detecting in high performance liquid chromatography, and calculating the contents of beta-nicotinamide nucleotide (NMN), beta-nicotinamide adenine dinucleotide (NAD), nicotinic acid (NA) and nicotinamide (NAM) in the to-be-detected sample according to a formula.

The invention relates to a composition with an eye enlarging effect and application of the composition. The composition is prepared from soluble collagen, tocopheryl nicotinate, acetyl hexapeptide-8, hydrolyzed rice protein, acetyl tetrapeptide-5, Arabic gum, rhizobium gum, an arnica extract and schizophyllum commune fermentation product filtrate. By compounding a plurality of plant extracts and polypeptides for use, excellent effects of tightening, lifting, removing pouches and reducing melanin can be realized, and the eye cream has a visual eye enlarging effect and is more young.

A composition comprising an active substance selected from nicotinamide; nicotinic acid; nicotinic acid esters; tryptophan; a tryptophan dipeptide; nicotinamide adenine dinucleotide (NAD); nicotinamide adenine dinucleotide phosphate (NADP); an intermediate in the biosynthesis of NAD or NADP selected from the group consisting of N-formylkynurenine, L-kynurenine, 3-hydroxy-L-kynurenine, 3-hydroxyanthranilate, 2-amino-3-carboxymuconate semialdehyde, quinolinate, nicotinic acid mononucleotide (beta-nicotinate D-ribonucleotide), and nicotinic acid adenine dinucleotide; nicotinamide riboside; nicotinamide mononucleotide; 1-methylnicotinamide (N-methylnicotinamide); or a combination thereof, for use for preventing and / or reducing one or more post-acute symptoms of an infection, wherein the composition is formulated to partly or completely release the active substance(s), preferably nicotinamide, for topical supplementation or efficacy in the lower small intestine and / or the colon, wherein preferably the pathogen is SARS-CoV-2 and / or the post-acute symptoms are PCS

The present disclosure relates to a non-therapeutic use of a human milk oligosaccharide (HMO) selected from 3'-sialyllacto-N-tetraose (LSTa), 6'-sialyllacto-N-neotetraose (LSTc) and 3'-sialyl- 3-fucosyllactose (FSL), or a combination thereof, in supporting or improving one or more of brain health, immune system health, gut health, and metabolic health, in a subject, optionally by increasing the production of one or more metabolites selected from the group consisting of of pyridoxine, pyridoxamine, nicotinic acid, N-acetyl-L-glycine, sarcosine, N-acetyl-L-glutamine, N- acetyl-L-aspartic acid, trigonelline, trans-4-hydroxyproline, 3-hydroxybutyric acid (BHB), 4- hydroxybenzoic acid, and N-acetyl-L-tryptophan, in the gastrointestinal tract of the subject.

The invention relates to the technical field of tobacco leaf grade discrimination, in particular to a metabolic marker composition for discriminating the grade of tobacco leaves, a screening method and application. The invention discloses a metabolic marker composition for judging the grade of tobacco leaves. Comprising the following metabolic markers: L-seryl-L-tyrosine, L-tryptophanyl-L-histidine, D-gluconic acid, N-acetylglutamic acid, linoleic acid, alpha-linolenic acid, nicotine, shikimic acid, N-methyl nicotinic acid, O-acetyl-L-carnitine, D-mannose, D-glucaric acid, phosphatidylcholine 36: 1, sucrose, mesaconic acid, fraxin and N-methyltryptamine. By utilizing the 17 metabolic markers, the grade of the tobacco leaves can be effectively distinguished.

The invention relates to the technical field of chemical synthesis, in particular to reaction equipment for preparing benzyl pyridinium nicotinate and a preparation process of the benzyl pyridinium nicotinate. The reaction equipment for preparing the benzyl pyridinium nicotinate comprises a heat preservation shell, a reaction kettle, a temperature control device and a temperature control device, and an installation space is formed in the heat preservation shell; the mixing cavity is arranged at the top of the mounting space, a feeding pipe is arranged at the top in the mixing cavity, and a discharging opening is formed in the bottom; the mixed reaction module comprises a feeding cavity, a mixed reaction pipe and a discharging cavity; the mixed reaction tube comprises a detection unit, a mixing unit and a verification unit; the collecting cavity is arranged below the mixing reaction pipe, is used for collecting and conveying the mixed liquid conveyed by the mixing reaction pipe, and is communicated with the mixing cavity and / or the feeding cavity through a conveying pipeline; and the bidirectional stirring mechanism is arranged in the mixing cavity and the collecting cavity. Under the condition that no catalyst is adopted, side reactions can be effectively inhibited, generation of by-products is reduced, the displacement and deep plating capacity is effectively improved, and the industrial value is good.

The invention relates to the technical field of chemical synthesis, in particular to a synthetic method of isoniazide, which comprises the following steps: carrying out cyanation reaction on 4-bromopyridine and K4Fe (CN) 6 under the catalytic action of a catalyst Pd / Fe3O4NPs to generate 4-cyanopyridine; 4-cyanopyridine is subjected to a hydrolysis reaction under the alkaline condition to generate isonicotinic acid; isonicotinic acid and hydrazine hydrate are subjected to an amidation reaction under the action of a condensing agent to generate isoniazide. The synthesis method of isoniazide has the advantages of simple operation, good repeatability and cost saving.

The invention discloses a preparation method and application of beta-nicotinamide mononucleotide, and belongs to the technical field of nucleotide synthesis. Tetraacetyl ribose and ethyl nicotinate are used as raw materials, a catalyst is added for a condensation reaction, the catalyst comprises a component A and a component B, the component A is mercaptosilane modified mesoporous silica loaded trimethylsilyl trifluoromethanesulfonate, and the component B is nitrogen-doped carbon nanotube loaded stannous chloride. And after the condensation reaction, carrying out ammonolysis deacetylation reaction, phosphorylation reaction and post-treatment to obtain the beta-nicotinamide mononucleotide. According to the catalyst provided by the invention, the component A and the component B are two catalytic components loaded by different carriers, so that the catalyst is favorably filtered and recycled, the catalyst residue is reduced, double activation of a glycosyl donor and a nucleophilic reagent can be realized by adjusting the ratio of the component A to the component B and through a synergistic effect, the forward proceeding of a reaction is promoted, and the catalyst has high selectivity and is suitable for industrial production. The method is suitable for the application field of cosmetics.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.