100 results about "Nicotinuric acid" patented technology

The invention relates to dexcamphanol or fenchol ester derivatives of 2-hydroxynicotinic acid and pharmaceutical application of the dexcamphanol or fenchol ester derivatives, and belongs to the technical field of biological medicine. The structural general formula of the derivative disclosed by the invention is # imgabs0 #, wherein R1 is selected from-H,-Cl, alkyl with 1 to 8 carbon atoms,-CF3,-CF2H,-CN,-SCH3,-OH,-CHO,-CH2OH,-CH2NH2,-COOH, halogenated phenyl or-NR4R5; r4 and R5 are independently selected from-H or-CH3; r2 is selected from-H, alkyl with 1-6 carbon atoms, and acyl or carbamoyl with 2-7 carbon atoms; r3 is selected from # imgabs1; # X is selected from-CH = or-N =. The derivative disclosed by the invention has a good inflammation inhibition effect; and under the condition of high concentration, the compound has lower cytotoxicity and is safer. The compound can be used for preparing medicines for treating inflammation and inflammation-related diseases such as cerebral apoplexy, neuropathic pain, depression and senile dementia.

The invention provides a diagnostic marker for metabolic syndrome and preclinical stage thereof, and the diagnostic marker or a plasma small molecule metabolism biomarker consists of the following substances: pyrimidine, (S)-beta-aminoisobutyric acid, 3-hydroxybutyric acid, L-serine, guanidine acetic acid, 3-hydroxyisovaleric acid, L-homoserine, L-cysteine, piperidine acid, hypotaurine and creatinine. The beverage is prepared from phenylacetic acid, erythritol, picolinic acid, taurine, pyroglutamic acid, lemonic acid, malic acid, tyramine, isonicotinic acid, o-phosphoethanolamine, L-glutamine, salicylic acid and D-glucose. The invention also provides a kit for detecting the plasma small molecule metabolism biomarker composition, the spectrum peak intensity of the obtained plasma small molecule metabolism biomarker is detected, the risk score of whether each subject is MetS or pre-MetS is calculated, and then whether the subject is MetS or pre-MetS is predicted. According to the invention, efficient and rapid diagnosis of the metabolic syndrome and the preclinical stage thereof is realized.

The invention belongs to the technical field of biological polypeptide research, and particularly relates to a polypeptide capable of removing active oxygen and increasing SIRT gene expression quantity and application of the polypeptide. The general formula of the sequence of the polypeptide is nicotinic acid-C-C-A-r-B-r-B-r-B-r-A-R-G-D-A-NH2, and the general formula of the sequence of the polypeptide is as follows: Wherein G is glycine, R is arginine, D is aspartic acid, and r is arginine; a is one of tryptophan, tyrosine and phenylalanine, B is one of tryptophan, phenylalanine, tyrosine and 3-cyclohexyl alanine, C is one of tryptophan, tyrosine and phenylalanine, A, B and C are different from one another, r is a D configuration, and other amino acids are L configurations. The polypeptide provided by the invention can significantly improve the expression of longevity protein related genes in HaCaT cells, and can effectively remove ROS in the cells, so that the polypeptide has a wide application prospect in the aspects of aging resistance and oxidation resistance.

This invention discloses a method for efficient genetic transformation of rice that overcomes genotype dependence and a dedicated culture medium, relating to the field of biotechnology. The dedicated culture medium comprises KCl, KH₂PO₄, MgSO₄·7H₂O, CaCl₂·2H₂O, disodium ethylenediaminetetraacetate, FeSO₄·7H₂O, MnSO₄·H₂O, ZnSO₄·7H₂O, H₃BO₃, KI, CoCl₂·6H₂O, CuSO₄·5H₂O, Na₂MoO₄·2H₂O, glutamine, aspartic acid, arginine, glycine, cyclohexanehexyl alcohol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, and a carbon source. Using this dedicated culture medium can increase the number of rice cells containing the NiR gene. 375 S 453 The genetic transformation efficiency of haplotype rice varieties is of great significance for overcoming the bottleneck of rice genotype dependence, promoting functional genomics research and molecular breeding.

The invention discloses a phagostimulant capable of improving the prevention and control effect of cotton flower thrips and application of the phagostimulant. The phagostimulant is prepared from the following components in parts by weight: 10 to 30 parts of p-methoxycinnamyl aldehyde, 5 to 40 parts of anisaldehyde, 40 to 70 parts of ethyl nicotinate and 10 parts of a cosolvent. The phagostimulant is prepared from the following components in parts by weight: 10 to 30 parts of p-methoxycinnamyl aldehyde, 5 to 40 parts of anisaldehyde, 40 to 70 parts of ethyl nicotinate and 10 parts of cosolvent. By utilizing the tropism of the cotton flower thrips to specific volatile compounds and through the synergistic effect of the three plant source compounds, pests hidden in stamens, petal wrinkles, leaf backs and other hidden parts are attracted to a pesticide application area, the contact area of insect bodies and the pesticide is remarkably increased, and therefore the prevention and control effect is improved. Field tests show that when an optimal formula (the ratio of p-methoxycinnamaldehyde to anisaldehyde to ethyl nicotinate to the cosolvent is 20: 20: 50: 10) is matched with spinetoram for use, the control effect can reach 84.43%-92.22%, the control effect is remarkably improved compared with that of single use of an insecticide, and a good control effect is shown. The method is safe and environment-friendly, reduces the amount and increases the efficiency, and has remarkable economic and ecological benefits.

This invention discloses a highly efficient catalytic synthesis process for nicotinamide intermediates using the field of organic synthesis technology. The process includes: adding appropriate amounts of dichloronicotinic acid, organic solvent, lanthanum-doped composite metal fluoride-diatomaceous earth supported catalyst, and encapsulated 4A molecular sieve to a reaction vessel under nitrogen protection; purging with nitrogen, heating, and stirring; introducing anhydrous dimethylamine gas, controlling the pressure, and continuing stirring; after the reaction is complete, washing with sodium bicarbonate aqueous solution, washing with deionized water, drying, concentrating, and recrystallizing to obtain the target product. The lanthanum-doped composite metal fluoride-diatomaceous earth supported catalyst prepared by this invention is recyclable, has mild reaction conditions, high product yield, is environmentally friendly, and suitable for industrial production.

This invention provides an induction culture medium and method for using marigold anthers as explants. The induction culture medium is based on MS solid medium containing agar, with specific amounts of sucrose, tryptophan, phenylalanine, arginine, casein amino acids, anhydrous asparagine, 6-benzylpurine, naphthaleneacetic acid, silver nitrate, pyridoxine hydrochloride, thiamine hydrochloride, nicotinic acid, D-biotin, folic acid, ascorbic acid, and coconut juice added. This invention also provides a method for induction culture using this induction culture medium. This invention can greatly improve the callus induction rate of marigolds, significantly increasing the number of regenerated seedlings, and provides technical guidance for the cultivation of haploid marigolds using anthers.

The invention belongs to the field of medicine synthesis, and relates to a preparation method of a raw material medicine, in particular to a preparation method of a nikethamide raw material medicine, which is prepared by four steps of salifying, condensing, neutralizing and washing, and oxidizing and steaming water to decolor. In the production of nikethamide, nikethamide is firstly salified, after the reaction activity of nikethamide is improved, nikethamide is further condensed with diethylamine, the reaction is an exothermic reaction, the reaction temperature is strictly controlled in the reaction process, the reaction rate of condensation is improved, and the yield is also improved; furthermore, through post-treatment such as oxidation, water distillation and decoloration, the produced raw material medicine has good characters and chemical stability, and meets the requirements of Chinese pharmacopoeia 2020 edition on nikethamide. According to the invention, the production process of the nikethamide bulk drug is optimized, the production period is shortened, and the cost is reduced.

The invention discloses a nutrition regulating agent for dairy cows in the perinatal period and a preparation method thereof, and relates to the technical field of animal husbandry production. The feed additive comprises the following nutritional ingredients in percentage by mass: 10%-20% of calcium carbonate, 5%-10% of calcium hydrophosphate, 10%-15% of rumen protected fatty acid calcium, vitamin D3, 4%-6% of anionic salt, 0.1%-0.15% of L-carnitine, 0.6%-1% of nicotinic acid, 0.08%-0.12% of tryptophan, 2%-3% of a yeast culture, 0.3%-0.7% of sodium butyrate and the balance of a carrier. The preparation method comprises the following steps: 1, premixing calcium carbonate, calcium hydrogen phosphate, rumen protected fatty acid calcium, anionic salt and vitamin D3; step 2, respectively coating metabolism adaptive components, namely L-carnitine, nicotinic acid and tryptophan, and functional additives, namely yeast culture and sodium butyrate, by micro-capsules to prepare the micro-capsules; and 3, mixing the premix obtained in the step 1, the microencapsulated component obtained in the step 2 and a carrier, and granulating at low temperature to obtain the nutrition regulating agent.

The present invention belongs to the technical field of biomedicine, and relates to a class of dexborneol or fenchyl alcohol ester derivatives of 2-hydroxynicotinic acid, and pharmaceutical uses thereof. The structural general formula of the derivatives is (1), R1 being selected from -H, -Cl, a hydrocarbon group having 1-8 carbon atoms, -CF3, -CF2H, -CN, -SCH3, -OH, -CHO, -CH2OH, -CH2NH2, -COOH, halogenated phenyl, or -NR4R5; R4, R5 are independently selected from -H or -CH3; R2 is selected from -H, an alkyl group having 1-6 carbon atoms, an acyl group having 2-7 carbon atoms, or a carbamoyl group; R 3 is selected from formula (a) or formula (b); X is selected from -CH= or -N=. The derivatives have good inflammation-inhibiting effects, and at high concentrations, they exhibit low cytotoxicity and are safer. The derivatives can be used for preparing a drug for treating inflammation and inflammation-related diseases such as stroke, neuropathic pain, depression and Alzheimer's disease.

The application belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for producing L-valine and a construction method and application thereof. The genetically engineered bacterium takes Escherichia coli as a host, heterologously overexpresses alss genes of Bacillus subtilis for coding acetyl-lactate synthetase and bcd genes for coding leucine dehydrogenase, brnFE genes of Corynebacterium glutamicum for coding branched-chain amino acid membrane transport proteins, and overexpresses ilvC genes of Escherichia coli for coding ketol-acid reductoisomerase mutants L67E / R68F / K75E, ilvD genes for coding dihydroxy-acid dehydratase, gapA genes for coding 3-phosphoglyceraldehyde dehydrogenase and pncB genes for coding nicotinate phosphoribosyltransferase; and does not express mdh genes for coding malate dehydrogenase; and replaces thioctamide dehydrogenase with a mutant A321T. A genetically engineered bacterium for stably and efficiently producing L-valine is obtained.

The present disclosure provides antibodies and polypeptides that specifically bind to nicotinic acid phosphoribosyltransferase (NAPRT). Also provided are nucleic acids encoding the antibodies, expression vectors and host cells for making the antibodies, methods of using the antibodies to detect NAPRT in a sample from a subject in need thereof, methods of using the antibodies to diagnose a subject having cancer or sepsis. Compositions comprising these antibodies are also provided. Also provided are compositions comprising a nicotinamide phosphoribosyltransferase inhibitor (NAMPTi), and methods of using the NAMPTi to treat cancer following diagnosis with the NAPRT antibody.

The invention discloses an efficient rice genetic transformation method capable of overcoming genotype dependence and a special culture medium, and relates to the technical field of biology. The special culture medium is prepared from KCl, KH2PO4, MgSO4. 7H2O, CaCl2. 2H2O, disodium ethylene diamine tetraacetate, FeSO4. 7H2O, MnSO4. H2O, ZnSO4. 7H2O, H3BO3, KI, CoCl2. 6H2O, CuSO4. 5H2O, Na2MoO4. 2H2O, glutamine, aspartic acid, arginine, glycine, inositol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid and a carbon source. By adopting the special culture medium, the genetic transformation efficiency of a rice variety containing the NiR gene E375S453 haplotype can be improved, and the special culture medium has important significance in overcoming the bottleneck of rice genotype dependence and promoting functional genome research and molecular breeding.

The application discloses a preparation method of a meta-aminobenzoic acid bisamide compound. The method is prepared by condensation reaction with N-(2-bromo-4-(perfluoropropane-2-yl)-6-(trifluoromethyl)phenyl)-2-fluoro-3-(hydroxyl amido)benzamide and 2-fluoropyridine-5-formyl chloride as raw materials. The 2-fluoropyridine-5-formyl chloride is prepared from 6-fluoronicotinic acid and phosgene. The preparation method takes the 2-fluoropyridine-5-formyl chloride with high yield and high purity as raw material, can omit the purification step, shorten the synthesis route, improve the preparation efficiency, improve the selectivity of the reaction, reduce the generation of impurities, improve the yield of the product, efficiently prepare the product with high yield and high purity, has the advantages of simple process, convenient operation, low cost, mild reaction condition, high preparation efficiency, high yield, high purity, environmental friendliness and the like, is suitable for large-scale preparation, and is convenient for industrial application.

The invention relates to the field of pesticide synthesis, in particular to a synthesis method of ethonicotinic acid. 5-ethyl pyridine-2. 3-dicarboxylic acid diethyl ester and 2-amino-2. 3-dimethyl butyramide are used as raw materials and react in a sodium ethoxide ethanol solution at the temperature of 50-70 DEG C, hydrogen chloride gas is introduced into a system for acidification after the reaction is completed, and imazethapyr and a by-product sodium chloride are obtained. The hydrogen chloride gas is directly introduced in the acidification process, and the acidification process is free of hydration, so that on one hand, no direct wastewater is generated, the generation of wastewater with high salt content and high organic matter content is avoided, the treatment cost is reduced, on the other hand, the byproduct sodium chloride is firstly removed from the system by controlling the ethanol amount of the system, and then concentration and crystallization are performed; the high-purity imazethapyr of which the purity is greater than 99% is obtained by utilizing the solubility of the solvent ethanol to impurities, and the quality of both byproducts and main products is at a higher level in the industry.

The present invention provides a preparation method of tozasertib, which relates to the technical field of medicine. The preparation method provided by the present invention comprises the following steps: (1) reacting methyl 2-cyanoisonicotinate with hydrazine hydrate, and crystallizing to obtain intermediate TPS-1; (2) reacting 4-cyanopyridine with a base, and then reacting completely with TPS-1, followed by filtration, washing and drying to obtain TPS-2a; (3) subjecting TPS-2a to a cyclization reaction with a reaction solvent, and after the reaction is complete, mixing with a crystallization solvent to obtain the product TPS. The product obtained by this process has a relatively high yield, with the highest total yield being about 62%, high purity, with a purity greater than 99.9%; the preparation method is simple, under the conditions of this preparation method, the raw materials react quickly, and at the same time, time-consuming operations such as extraction and concentration are avoided, and the product can be directly obtained by filtration after the reaction, with a short production cycle; the reaction conditions are easy to realize industrial operation.

To provide formulation technology with which it is possible to suppress browning that occurs over time in a topical composition containing a non-steroidal anti-inflammatory, 0.015 wt.% or more of vanillylamide nonanoate, and 0.01 wt.% or more of benzyl nicotinate.SOLUTION: In this topical drug composition, the browning that occurs over time can be suppressed effectively by blending a monoterpene with a non-steroidal anti-inflammatory, 0.015 wt.% or more of vanillylamide nonanoate, and 0.01 wt.% or more of benzyl nicotinate.SELECTED DRAWING: None

The application discloses a composition for improving anti-aging and firming effects and a preparation method thereof, and belongs to the technical field of cosmetics. The composition for improving anti-aging and firming effects comprises the following raw materials in parts by mass: galactomyces ferment l filtrate 20-60 parts, nicotinamide adenine dinucleotide 0.1-5 parts, myristyl nicotinate 0.5-5 parts, chrysanthemum coronarium leaf extract 0.2-4.5 parts, lens culinaris seed extract 0.1-5.5 parts and chrysanthemum coronarium extract 0.3-5 parts. The composition for improving anti-aging and firming effects has significant firming and anti-aging effects and is overall mild and non-irritating.

The application provides a preparation method of a medical intermediate 4-bromo-6-chloronicotinic acid methyl ester. The method takes 4,6-dichloronicotinic acid methyl ester as a starting material, reacts with an alkali salt to obtain 6-chloro-4-hydroxynicotinic acid methyl ester; then the 6-chloro-4-hydroxynicotinic acid methyl ester reacts with a bromination reagent under the action of an acid binding agent to obtain the target product 4-bromo-6-chloronicotinic acid methyl ester. The preparation method has short steps, the raw material is easy to obtain and low in price, the reaction conditions are mild, easy to purify, and simple to operate; meanwhile, the product has high yield, high purity, low production cost, is convenient for industrialized production, has a good application prospect, and plays a positive role in the research and development of downstream products of 4-bromo-6-chloronicotinic acid methyl ester.

This invention discloses a 2-hydroxynicotinic acid-2-hydroxybenzyl ester, its preparation method, and its application. The structural formula of the 2-hydroxynicotinic acid-2-hydroxybenzyl ester is shown below. This invention utilizes 2-hydroxynicotinic acid as a raw material and obtains a 2-hydroxynicotinic acid-2-hydroxybenzyl ester with a novel structure through esterification. The 2-hydroxynicotinic acid-2-hydroxybenzyl ester has low cytotoxicity, can scavenge reactive oxygen species, and has good anti-UVB-induced oxidative damage activity in HaCaT cells. It can be used in products for anti-skin oxidative damage.

The present application relates to the technical field of oral liquid, in particular to a kind of oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow and preparation method thereof, it is characterized in that, the oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow is made of the following components, by weight parts: artemisia capillaris 200~300 parts, gardenia 150~200 parts, bupleurum 100~150 parts, polygonatum 50~100 parts, radix paeoniae alba 50~100 parts, liquorice 10~50 parts, nicotinic acid 1~10 parts, propylene glycol 200~300 parts, fructose diphosphate sodium 1~10 parts, pure water 200~400 parts.The oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow can prevent and treat ketosis, fatty liver and liver damage at the same time, comprehensively improve the constitution of new cow, improve immunity, improve physical indicators and reproductive performance, increase economic benefits.

Disclosed in the present invention are a salt of a nicotinate compound, a crystal of the salt, and a preparation method therefor and a use thereof. The salt is selected from benzenesulfonate, phosphate, sulfate, hydrochloride, and methanesulfonate of a compound represented by formula (I). The crystal is crystal form A of the benzenesulfonate of the compound represented by formula (I), crystal form B of the benzenesulfonate of the compound represented by formula (I), crystal form A of the phosphate of the compound represented by formula (I), crystal form A of the sulfate of the compound represented by formula (I), crystal form A of the hydrochloride of the compound represented by formula (I), crystal form A of the methanesulfonate of the compound represented by formula (I), crystal form B of the methanesulfonate of the compound represented by formula (I), or an amorphous form of the methanesulfonate of the compound represented by formula (I).

The present invention relates to a method for controlling the fruit hair of the plane tree, comprising: a. drilling an injection hole at the primary bifurcated branch of the plane tree, the drilling time of the injection hole is the end of March-mid-April or mid-April-early May each year; b. inserting a device for controlling the fruit hair of the plane tree into the injection hole, the device comprising a medicament composed of the following components by mass percentage: 1%-5% ethephon, 0.5%-1.5% fructose, 0.05%-0.15% nicotinic acid, and the balance is water. The method of the present invention is simple to operate, has a good fruit hair control effect, and the removal rate of the plane tree fruit is as high as 99%; the medicament used in the present invention, whether it is a low-concentration medicament or a high-concentration medicament, is within an acceptable range for the damage to the plane tree body; the device used in the present invention for controlling the fruit hair of the plane tree can effectively ensure that the medicament flows out smoothly without overflowing, and can accurately calculate the dosage of the medicine.

The invention discloses a preparation method of 4-bromo-6-chloronicotinic acid, which is characterized by comprising the following steps: dissolving 2-chloro-4-amino-5-methylpyridine in dilute acid, then adding a catalyst and a bromide, and dropwise adding a sodium nitrite aqueous solution to diazotize the bromine to obtain 2-chloro-4-bromo-5-methylpyridine, wherein the molar ratio of the 2-chloro-4-amino-5-methylpyridine, the catalyst, the bromide and the sodium nitrite is 1:(1-3):(2-5):(2-5); and (2) reacting the 2-chloro-4-bromo-5-methylpyridine with an oxidizing agent to obtain 4-bromo-6-chloronicotinic acid, wherein the molar ratio of the 2-chloro-4-bromo-5-methylpyridine to the oxidizing agent is 1:(1-3). The method has the advantages of simple process circuit, mild and safe reaction conditions, low production cost and high total yield.

The invention provides a two-step continuous preparation method of 2-chloro-N, N-dimethyl nicotinamide, which comprises the following steps: mixing 2-chloronicotinic acid with a solvent and thionyl chloride in proportion to prepare a solution A; continuously adding the solution A into the first section of the tubular reactor at a certain flow through a metering pump, and controlling the reaction temperature and the retention time to prepare a solution B; and continuously feeding the solution B into a tubular reactor II, continuously adding dimethylamine into the tubular reactor II, controlling the pressure, the temperature and the retention time, carrying out an amide reaction in the tubular reactor, and carrying out solid-liquid separation by using a centrifugal machine after the reaction is completed to obtain a liquid which is a 2-chloro-N, N-nicotinamide solution. According to the technical scheme, the compatible organic matter is used for replacing a large amount of thionyl chloride to serve as the reaction solvent, two-step continuous reaction is achieved, the procedure of distilling excessive reactants after acyl chloride is prepared is omitted, and the technological process is simplified.

The invention relates to metabonomic characterization of microorganisms, in particular to a method for identifying cell types of cells in a sample, comprising: culturing the sample in a growth medium comprising nicotinamide to obtain a cultured growth medium; analyzing the cultured growth medium by chemical analysis; and identifying the cell type as at least one of the genus Escherichia, the genus Klebsiella, the genus Pseudomonas, the genus Enterococcus, the genus Staphylococcus or the genus Streptococcus when the cultured growth medium contains a higher concentration of nicotinate than the growth medium; wherein the growth culture medium comprises the following components in a buffer culture medium: 0.02 to 2 g / l of D-glucose, 0.0001 to 0.1 g / l of nicotinamide, 0.0001 to 0.1 g / l of pyridoxin.HCl, 0.02 to 1 g / l of hypoxanthine, 0.2 to 5 g / l of L-arginine, 0.2 to 5 g / l of L-threonine, 0.1 to 5 g / l of L-histidine, 0.02 to 2 g / l of spermine and catalase. According to the method, identification and toxin sensitivity detection can be completed within about half of time compared with that of an existing method.

The subject of the application is a tool for applying methyl nicotinate solution, which is characterized in that it consists of a base (1) in the shape of a cuboid. The lower surface of the base (1) has a T-shaped recess along its entire length, in which at least two T-shaped mounting brackets (2) are press-fitted. Square frames (3) are attached to the lower surfaces of the mounting brackets (2). Sponges (4) are press-fitted into the frames (3). A digital timer (5) is attached to the upper surface of the base (1) by gluing.