The invention discloses a culture medium for separating actinomycetes in a high-salt environment and application thereof. The culture medium is composed of 1% of mannitol, 0.1% of alanine, 0.1% of K2HPO4, 0.1% of MgSO4.7H2O, 0.02% of CaCO3, 0.001% of FeSO4.7H2O, 1.8% of Agar, 0.0003% of trace salt, 0.0004% of compound vitamin, 0.000025% of biotin and 0.005% of potassium dichromate, wherein the trace salt is composed of 0.0001% of FeSO4.7H2O, 0.0001% of MnCl2.4H2O and 0.0001% of ZnSO4.7H2O; and the compound vitamin is composed of 0.00005% of vitamin B1, 0.00005% of vitamin B2, 0.00005% of vitamin B6, 0.00005% of nicotinic acid, 0.00005% of inositol, 0.00005% of pantothenic acid, 0.00005% of paraaminobenzoic acid and 0.00005% of folic acid. The culture medium is suitable for separating actinomycetes in an oligotrophic high-salt environment which has a high salt content, and has wide application values.