38results about How to "Separation fit" patented technology

The invention relates to the technical field of aspergillus oryzae isolation and preservation, in particular to a simple method for isolating and preserving aspergillus oryzae. Its separation method comprises the following steps: collect fresh yellow, green and black rice bent ball samples from diseased paddy fields, and dry at room temperature for 7-10d for subsequent use; select chlamydospore powder from the collected samples to cover rice bent rice with a thickness greater than 1mm Put the ball on the ultra-clean workbench and sterilize it with ultraviolet light for 30-40 minutes; take a single rice ball with tweezers under sterile conditions, and gently shake off a small amount of chlamydospore powder on the surface of the rice ball and put it on the PSA medium On; use a pipette gun to take 150-200ul sterile water drops on the PSA plate containing chlamydospores and so on. The method of the present invention can be used to quickly isolate and store the target bacterial strain, which solves the problems of time-consuming and low efficiency in the isolation of Aspergillus oryzae, and also solves the problems of pathogenicity degradation and space consumption of the bacterial strain during storage. .

The invention relates to an online low-pressure, middle-pressure and high-pressure combined two-dimension preparation liquid chromatographic system. Two one-dimension low-pressure, middle-pressure and high-pressure preparation liquid chromatographic systems are organically combined through a six-way valve and software. Pressure used by a second dimension system is not lower than pressure used by a first dimension separation system. When the first dimension system and the second dimension system are low-pressure or middle-pressure preparation liquid chromatographic systems, through switching the six-way valve, target compound directly enters a second dimension chromatographic column for second dimension separation after first dimension separation; and when pressure used by a second dimension separation system is higher than pressure used by the first dimension separation system, a trapping column is connected on the six-way valve, the target compound is adsorbed on the head of the trapping column after the first dimension separation, and then the target compound enters the second dimension chromatographic column for second dimension separation. The invention has the advantages thatthe contradiction between sample injection volume and separation capacity is solved, the large sample injection volume is ensured and the separation capacity of the preparation chromatographic systemis greatly improved; the system structure is simple and the establishment is easy; the operation is simple and convenient and the automation can be easily realized; and the application scope is wide.

The invention discloses a separation device and method for a mixture of gas, water and coal, comprising a separation bin, the separation bin is provided with a feed port, a pulverizer is installed on the feed port, and the separation bin A gas delivery pipe is installed on the upper end of the gas delivery pipe, and a gas collecting bottle is installed on the gas delivery pipe; a first screen is installed in the separation bin, and a first coal discharge pipe is installed on the first screen, and the first coal discharge pipe is installed on the first screen. The first row of coal pipes protrudes from the separation bin, and a vibrator is installed on the first screen, the vibrator is vertically arranged, and a sound wave transducer is installed at the lower end of the vibrator. A vibrating motor and an ultrasonic generator are installed outside the separation chamber, and the vibrating motor is connected with a vibrator. The separation device of the invention is simple in structure, safe and reliable, and is suitable for the separation of gas, water and coal mixtures.