Sandwich immunoassay kit

A sandwich immunization and kit technology, applied in the field of sandwich immunization kits, can solve the problem that the detection method is insufficient to meet the sensitivity, and achieve the effects of rapid determination, enhanced Raman signal, and strong Raman signal.

Pending Publication Date: 2021-12-21
BOZHOU CITY THE NEW HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0028] At present, the commonly used detection methods for the whole CRP are immunodiffusion method, radioimmunoassay, turbidity method, and enzyme-labeled immunoassay method. The detection limit of the above methods is μg. The normal range is 800-8000μg/L (immunodiffusion or turbidity method), but the prevention detection

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Examples

Experimental program
Comparison scheme
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Embodiment 1

[0090] Such as figure 1 Shown is a process diagram of the structure formation of a sandwich immunoassay kit of the present invention. This embodiment provides a preparation method of a sandwich immunoassay kit. The method includes the following steps:

[0091] 1. Preparation of gold-core silver-shell nanorods

[0092] 1. Preparation of gold nanorods

[0093] 1) In a 20mL glass bottle, dilute 0.1mL of 25mM HAuCl4 solution with deionized water to 5mL, and add 5mL of 0.2M CTAB solution to the diluted solution to obtain solution 1;

[0094] 2) Quickly inject 0.6mL of 0.01 M NaBH4 solution into Solution 1. The NaBH4 solution is ready-to-use, and the mixed solution is magnetically stirred at a speed of 1200 rpm for 2 minutes. The finally obtained seed solution is kept at 30°C for 30 minutes to be use;

[0095] 3) Dissolve 7.0g of CTAB and 1.234g of sodium oleate in 250mL of 50°C water, cool naturally to 30°C, then add 18ml of 4.0mM silver nitrate solution and keep warm for 1min t...

Embodiment 2

[0135] In this example, a sandwich immunoassay kit is used to detect β2-microglobulin. This embodiment provides a method for preparing a sandwich immunoassay kit, which method includes the following steps:

[0136] 1. Preparation of gold-core silver-shell nanorods

[0137] 1. Preparation of gold nanorods

[0138] 1) In a 20mL glass bottle, dilute 0.1mL of 25mM HAuCl4 solution with deionized water to 5mL, and add 5mL of 0.2M CTAB solution to the diluted solution to obtain solution 1;

[0139] 2) Quickly inject 0.6mL of 0.01 M NaBH4 solution into Solution 1. The NaBH4 solution is ready-to-use, and the mixed solution is magnetically stirred at a speed of 1200 rpm for 2 minutes. The finally obtained seed solution is kept at 30°C for 30 minutes to be use;

[0140] 3) Dissolve 7.0g of CTAB and 1.234g of sodium oleate in 250mL of 50°C water, cool naturally to 30°C, then add 18ml of 4.0mM silver nitrate solution and keep warm for 1min to obtain solution 2;

[0141] 4) Inject 250ml...

Embodiment 3

[0180] In this example, a sandwich immunoassay kit is used to detect heart-type fatty acid binding protein. This embodiment provides a method for preparing a sandwich immunoassay kit, which method comprises the following steps:

[0181] 1. Preparation of gold-core silver-shell nanorods

[0182] 1. Preparation of gold nanorods

[0183] 1) In a 20mL glass bottle, dilute 0.1mL of 25mM HAuCl4 solution with deionized water to 5mL, and add 5mL of 0.2M CTAB solution to the diluted solution to obtain solution 1;

[0184] 2) Quickly inject 0.6mL of 0.01 M NaBH4 solution into Solution 1. The NaBH4 solution is ready-to-use, and the mixed solution is magnetically stirred at a speed of 1200 rpm for 2 minutes. The finally obtained seed solution is kept at 30°C for 30 minutes to be use;

[0185] 3) Dissolve 7.0g of CTAB and 1.234g of sodium oleate in 250mL of 50°C water, cool naturally to 30°C, then add 18ml of 4.0mM silver nitrate solution and keep warm for 1min to obtain solution 2;

...

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Abstract

The invention discloses a sandwich immunoassay kit. The invention provides an application of a surface-enhanced Raman detection kit in creatine kinase isoenzyme detection, the kit comprises a top layer reagent, a bottom layer reagent, a BSA solution and an EDTA anticoagulant, the top layer reagent in the kit adopts 3-mercapto-1, 2, 4-triazole molecules as Raman detection molecules, and compared with common P-ATP molecules in the prior art, the 3-mercapto-1, 2, 4-triazole molecules have a stronger Raman signal enhancing function; the surface-enhanced Raman detection kit is applied to the detection of creatine kinase isoenzyme in a human body for the first time, can make up for the defects in the existing detection technology, greatly improves the detection limit and sensitivity, and plays a key role in preventing sepsis.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a sandwich immune kit. Background technique [0002] Neutrophil gelatinase-associated lipocalin (NGAL) is a kind of lipocalin, also known as human lipocalin 2 (lipocalin2, Ln2) or siderocalin (siderocalin), is a member of the human lipocalin family A new member, a small-molecular-weight secreted protein originally discovered in activated neutrophils, can participate in the operation of iron-containing substances, and its expression level in vivo is an effective indicator for the treatment and monitoring of different kidney diseases, promoting Cell proliferation and inhibition of apoptosis after kidney injury. NGAL can induce renal tubular epithelial cell regeneration during renal injury. Modern studies have shown that NGAL is one of the most effective biological markers for the diagnosis of acute kidney injury and one of the effective markers for early diabetic nephropathy....

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/65G01N33/68
CPCG01N33/6893G01N33/577G01N33/587G01N2333/47G01N2800/347G01N2800/042G01N21/65G01N33/54346G01N33/573G01N33/6887G01N33/74G01N2333/4712G01N2333/4737G01N2333/58G01N2333/585G01N2333/805G01N2333/9123G01N2800/26G01N2800/323G01N2800/324G01N2800/325G01N2800/52G01N2800/7095G01N33/54313G01N33/68
Inventor 熊良钟熊清爵李庆海张茂峰熊孟智王梓光张超卫颜亚伟曹珍利
Owner BOZHOU CITY THE NEW HEALTH TECH CO LTD
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