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Creatine Kinase Isoenzyme Assay Kit

A technology of creatine kinase and isoenzyme, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high price and detection cost of immune turbidimetric kits, high cost of latex manufacturing, and unfavorable cleaning of biochemical instruments. The effect of high specificity, wide linear range and high stability

Active Publication Date: 2022-03-18
江西英大生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, latex-enhanced immunoturbidimetric reaction will produce precipitation, which is not conducive to the cleaning of the biochemical instrument and interferes with the test results. Moreover, the manufacturing cost of latex is high, resulting in high prices of immunoturbidimetric kits and detection costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of the first reagent: Weigh 18g tris buffer solution, 13gNaCl, 9gPEG 8000, 15g Tween 80, 1.5g sodium azide, 0.5g heparin, dissolve in 1.0L double distilled water, adjust the pH to 7.2, Dilute to 1.5L to get the reagent. The first reagent (30mL / dose):

[0026] (2) Prepare the second reagent: Dilute the polystyrene latex particles (average particle diameter 350nm) with carboxyl functional groups on the surface to 1% (w / v) with glycine buffer solution, and make the concentration 0.1% to 1% (w / Add the EDAC aqueous solution of v) to the granules, and react in a constant temperature shaker at 37°C for 30 minutes; dilute the creatine kinase isoenzyme antibody (mouse monoclonal antibody) to 0.5mg / ml with a glycine buffer solution, and add it to the granules In a constant temperature shaker, react at 37°C for 3 hours; add blocking agent, and place overnight at room temperature; add cleaning solution to wash and centrifuge the particles sealed overnight for 3 tim...

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Abstract

The invention discloses a creatine kinase isoenzyme (CKMB) assay kit, comprising a first reagent and a second reagent, wherein the first reagent is a phosphate buffer containing polyethylene glycol and heparin; the second reagent is a phosphate buffer containing polyethylene glycol and heparin; Creatine Kinase Isoenzyme Latex Antibody and Hyaluronic Acid in Glycine Buffer. The creatine kinase isoenzyme assay kit of the present invention can quantitatively and objectively reflect the existence of CKMB, and provide more powerful experimental diagnosis basis for clinical diagnosis, curative effect observation and prognosis judgment. The creatine kinase isozyme assay kit provided by the invention has high specificity, good repeatability, high detection sensitivity, wide linear range and high stability.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a creatine kinase isozyme assay kit, in particular to a creatine kinase isozyme assay kit for latex immune turbidimetry and an application thereof. Background technique [0002] Creatine kinase (CreatineKinase, CK) widely exists in various tissues and is related to the regeneration of adenosine triphosphate (ATP). Creatine kinase is a dimer composed of M and B subunits, and there are three isozymes in the cytoplasm: CK-MM, CK-MB and CK-BB. There is another isoenzyme called CK-Mt in the mitochondria of cells. Although these isoenzymes have the same relative molecular mass, they have different immune characteristics. Creatine kinase isoenzymes are of great significance in clinical diagnosis. When various lesions including muscle atrophy and myocardial infarction occur, the level of creatine kinase in human serum increases rapidly. It is currently consider...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 曾曜龙易剑峰施清蓉蔡其浩胡小雄
Owner 江西英大生物技术有限公司
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