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Anti-creatine kinase isoenzyme antibody as well as preparation method, application and amino acid sequence thereof

A technology of creatine kinase, amino acid, applied in the field of immunology

Active Publication Date: 2020-09-01
杭州博岳生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, if a monoclonal antibody against creatine kinase isoenzyme with high specificity and strong affinity can be developed and applied to the clinical diagnosis of acute myocardial infarction, the success rate of acute myocardial infarction can be greatly improved; No

Method used

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  • Anti-creatine kinase isoenzyme antibody as well as preparation method, application and amino acid sequence thereof
  • Anti-creatine kinase isoenzyme antibody as well as preparation method, application and amino acid sequence thereof
  • Anti-creatine kinase isoenzyme antibody as well as preparation method, application and amino acid sequence thereof

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Experimental program
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Effect test

Embodiment 1

[0122] Such as figure 2 Amino acid sequences of the indicated anti-creatine kinase isozyme antibodies, including

[0123] the amino acid sequence of the variable region of a heavy chain, comprising the sequence of SEQ ID NO: 1; and

[0124] the amino acid sequence of the variable region of a light chain, comprising the sequence of SEQ ID NO: 2,

[0125] The sequence of SEQ ID NO: 1 has at least one substitution with the sequence of SEQ ID NO: 2.

[0126] Instead of being selected from the group,

[0127] The 12th amino acid of SEQ ID NO: 1 is replaced by arginine by leucine, the 20th amino acid of SEQ ID NO: 1 is replaced by isoleucine by leucine, and the heavy chain obtained after the substitution is SEQ ID N0: 3;

[0128] The 6th amino acid of SEQ ID NO: 2 is replaced by glutamine to aspartic acid, the 12th amino acid of SEQ ID NO: 2 is replaced by serine to threonine, the 22nd amino acid of SEQ ID NO: 2 is replaced by Threonine is substituted with methionine, and the ...

Embodiment 2

[0131] Amino acid sequences of anti-creatine kinase isozyme antibodies, including

[0132] the amino acid sequence of the variable region of a heavy chain, comprising the sequence of SEQ ID NO: 1; and

[0133] the amino acid sequence of the variable region of a light chain, comprising the sequence of SEQ ID NO: 2,

[0134] The sequence of SEQ ID NO: 1 has at least one substitution with the sequence of SEQ ID NO: 2.

[0135] Instead of being selected from the group,

[0136] The 11th amino acid of SEQ ID NO: 1 is substituted by methionine to tryptophan, the 24th amino acid of SEQ ID NO: 1 is substituted by phenylalanine to proline, and the heavy chain obtained after the substitution is SEQ ID NO :5;

[0137] The 13th amino acid of SEQ ID NO: 2 is replaced by aspartic acid by alanine, the 21st amino acid of SEQ ID NO: 2 is replaced by arginine by methionine, the 42nd of SEQ ID NO: 2 amino acid is replaced by glutamic acid by serine, and the light chain obtained after the sub...

Embodiment 3

[0140] Amino acid sequences of anti-creatine kinase isozyme antibodies, including

[0141] the amino acid sequence of the variable region of a heavy chain, comprising the sequence of SEQ ID NO: 1; and

[0142] the amino acid sequence of the variable region of a light chain, comprising the sequence of SEQ ID NO: 2,

[0143] The sequence of SEQ ID NO: 1 has at least one substitution with the sequence of SEQ ID NO: 2.

[0144] Instead of being selected from the group,

[0145] The 22nd amino acid of SEQ ID NO: 1 is replaced by cysteine ​​with serine, the 50th amino acid of SEQ ID NO: 1 is replaced by leucine with glutamic acid, and the heavy chain obtained after the substitution is SEQ ID NO: 7;

[0146] The 35th amino acid of SEQ ID NO: 2 is replaced by aspartic acid by alanine, the 35th amino acid of said SEQ ID NO: 2 is replaced by tyrosine to glycine, the 35th amino acid of said SEQ ID NO: 2 68 amino acids were substituted by threonine for tryptophan, and the light chain ...

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Abstract

The invention relates to the technical field of immunology, and particularly discloses an anti-creatine kinase isoenzyme antibody and a preparation method, application and amino acid sequence thereof.The anti-creatine kinase isoenzyme antibody comprises an amino acid sequence of a variable region of a heavy chain, wherein the amino acid sequence comprises a sequence shown in SEQ ID NO: 1; and anamino acid sequence of a variable region of a light chain, wherein the amino acid sequence comprises a sequence shown in SEQ ID NO: 2. The sequence shown as SEQ ID NO: 1 and the sequence shown as SEQID NO: 2 have at least one substitution. The antibody has the characteristics of high specificity and strong affinity.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to an anti-creatine kinase isoenzyme antibody and its preparation method, application and amino acid sequence. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) (ATP: Creatine N-phosphotransferase EC2.7.3.2), also known as phosphocreatine kinase (Creatine Phosphokinase, CPK), usually exists in the cytoplasm of animal heart, muscle and brain tissues And in mitochondria, it can reversibly catalyze the transphosphoryl reaction between creatine and ATP, and is an important kinase directly related to intracellular energy operation, muscle contraction, and ATP regeneration. Creatine kinase is a dimeric protein composed of two subunits, which can be divided into muscle type (M), brain type (B), hybrid type (MB) and mitochondrial type according to the location of tissue distribution and subunit composition. Type (Mt) four isozyme forms. The muscle creatine kinase mole...

Claims

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Application Information

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IPC IPC(8): C07K16/40C07K1/36C07K1/30C07K1/22C12N15/13C12N15/85G01N33/573G01N33/577
CPCC07K16/40C12N15/85G01N33/573G01N33/577C07K2317/56G01N2333/9123G01N2800/324
Inventor 李因来戚跃明王玮
Owner 杭州博岳生物技术有限公司
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