Kit for determining activity of creatine kinase isoenzyme and determination method thereof

A technology of creatine kinase and assay method, which is applied in the field of kits for assaying creatine kinase isoenzyme activity and its assay field, and can solve the problems of reduced detection accuracy and the like

Pending Publication Date: 2021-12-31
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved in the embodiment of the present application is that in the related art, the detection sensitivity is improved by increasing the sample size, resulting in a decrease in detection accuracy.

Method used

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  • Kit for determining activity of creatine kinase isoenzyme and determination method thereof
  • Kit for determining activity of creatine kinase isoenzyme and determination method thereof
  • Kit for determining activity of creatine kinase isoenzyme and determination method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] In this embodiment, the first reagent R1 and the second reagent R2 were prepared according to the components and concentrations in Table 2 below.

[0069] Table 2 The composition and concentration table of the first reagent R1 and the second reagent R2

[0070]

[0071]

[0072] The preparation method of the above reagent is a conventional method, that is, the components of the reagent are added to purified water, mixed and stirred uniformly, and the pH is adjusted.

Embodiment 2

[0074] In this embodiment, the first reagent R1 and the second reagent R2 were prepared according to the components and concentrations in Table 3 below.

[0075] Table 3 The composition and concentration table of the first reagent R1 and the second reagent R2

[0076]

[0077]

[0078] The preparation method of the above reagent is a conventional method, that is, the components of the reagent are added to purified water, mixed and stirred uniformly, and the pH is adjusted.

[0079] The present application also sets up a set of comparative examples, and prepares the reagents R1 and R2 of the comparative examples according to the components and concentrations in Table 4 below.

[0080] Table 4 The composition and concentration table of the first reagent R1 and the second reagent R2

[0081]

[0082] The components of the kit of the control example are relative to the diaphorase and tetrazolium salt of Example 1, and the preparation method is the same as that of Example...

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Abstract

The embodiment of the invention belongs to the field of medical examination and determination, and relates to a kit for determining activity of creatine kinase isoenzyme. The kit comprises a first reagent and a second reagent; the first reagent comprises a first buffer solution, adenosine diphosphate, disodium ethylene diamine tetraacetate, magnesium acetate, an activator, adenosine monophosphate, an anti-human CK-MM antibody, AP5A, glucose, NAD (P) + and a first preservative; and the second reagent comprises the following components: a second buffer solution, phosphocreatine, magnesium acetate, hexokinase, glucose-6-phosphate dehydrogenase, tetrazolium salt, diaphorase and a second preservative. The invention also relates to a determination method of creatine kinase isoenzyme activity. According to the technical scheme, the test sensitivity can be improved.

Description

technical field [0001] The present application relates to the technical field of medical testing and determination, and more specifically, relates to a kit for determining the activity of creatine kinase isoenzyme and a determination method thereof. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) has three main isozymes, namely CK-MM, CK-MB, CK-BB. The muscle type (MM) mainly exists in various muscle cells, the brain type (BB) mainly exists in brain cells, the hybrid type (MB) mainly exists in cardiomyocytes, and the creatine kinase MB isoenzyme (CK-MB ) is the most specific enzyme in the myocardial enzyme spectrum. Creatine kinase MB isoenzyme (CK-MB) is mainly distributed in the myocardium, and its content changes can reflect the state of the myocardium. It can be used for the diagnosis and prognosis of acute myocardial infarction (AMI), diagnosis and prognosis of myocarditis, diagnosis of muscle diseases, brain Diagnosis of diseases such as cerebral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/9123
Inventor 齐文闯蒋析文吴润锋简俊兴段少卿卢华丹
Owner DAAN GENE CO LTD
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