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Method for determining triterpenes content of ganoderma lucidum spore oil

A technology of Ganoderma lucidum spore oil and substance content, which is applied in the direction of color/spectral characteristic measurement, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve inaccurate results and human body damage. , Chloroform is volatile and other problems, to achieve the effect of good reproducibility, stable method and strong reaction specificity

Inactive Publication Date: 2007-10-24
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some methods for using vanillin as the color reagent to directly determine the content of triterpenoids in Ganoderma lucidum products by colorimetry, such as the method of Yu Cun et al. (see references: Yu Cun, Yu Sha, Han Jianlong, etc. Ganoderma lucidum Study on the determination method of triterpenoids in health food. Zhejiang Preventive Medicine, 2006, 18(8): 74-75.) Indirect determination of triterpenes in Ganoderma lucidum products by colorimetry using vanillin as color reagent under the action of sulfuric acid The content of triterpenoids in Ganoderma lucidum spore oil is refluxed with chloroform, and both the reference substance and the test solution are fixed to volume with chloroform. Because chloroform is poisonous, it may cause damage to the human body, and chloroform is very volatile. When the liquid is used, the volume error is large, resulting in inaccurate results

Method used

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  • Method for determining triterpenes content of ganoderma lucidum spore oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Maximum Absorption Wavelength Determination

[0016] Accurately draw 1 mL of oleanolic acid solution into a colorimetric tube (make a blank at the same time), heat in a water bath to evaporate the solvent, add (vanillin-glacial acetic acid-perchloric acid) solution 1.0 mL, and heat in a water bath at 70°C for 15 minutes. Take out ice water and cool for 10 minutes, add 5 mL of glacial acetic acid to dilute, shake well, scan with a UV-visible spectrophotometer in the range of 500-600 nm, and measure the maximum absorbance value at 549 nm. The absorption curve is shown in Figure 1.

Embodiment 2

[0017] Embodiment 2: preparation of oleanolic acid standard curve

[0018] Preparation of standard solution: Accurately weigh 10 mg of oleanolic acid reference substance, dissolve in ethanol and dilute to 100 mL.

[0019] Precisely draw standard solutions 0.2, 0.4, 0.6, 0.8, 1.0, 1.2mL into colorimetric tubes (make a blank at the same time), heat in a water bath to evaporate the solvent, add vanillin-glacial acetic acid-perchloric acid solution 1.0mL, 70°C Heat in a water bath for 15 minutes, take out ice water and cool for 10 minutes, add 5 mL of glacial acetic acid to dilute, shake well, measure the absorbance value Y at 549 nm with a UV-visible spectrophotometer, the standard curve of oleanolic acid is shown in Figure 2, and the linear regression is calculated The equation is: Y=8.6018X-0.0011 and the correlation coefficient is R 2 = 0.9992.

Embodiment 3

[0020] Embodiment 3: Ganoderma lucidum spore oil sample determination

[0021] Accurately weigh 0.0302g of 1 drop of Ganoderma lucidum spore oil, add ethanol, shake, heat in a hot water bath at 60-70°C, shake to dissolve at the same time, and set the volume to 50mL to obtain the test solution. Measured according to Example 3, with the measured absorbance value Y through the linear regression equation, the result calculated by formula (1) is 14.6% of the mass fraction.

[0022] Precisely draw 1mL of the test solution into the colorimetric tube, heat in a water bath to evaporate the solvent (make a blank at the same time), add 1.0mL of vanillin glacial acetic acid perchloric acid solution, heat in a water bath at 70°C for 15 minutes, take out ice water and cool for 10 minutes, Add 5 mL of glacial acetic acid to dilute, shake well, and measure the absorbance at 549 nm with a UV-Vis spectrophotometer. With the measured absorbance value Y(A) through the linear regression equation,...

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Abstract

The invention relates to a method for measuring the content of triterpenoids in lucid ganoderma spore oil, which comprises that heating lucid ganoderma spore oil via alcohol in hot water bath at 60-70Deg. C until being dissolved, extracting the triterpenoids in the lucid ganoderma spore oil, to react with vanillin via perchloric acid in glacial acetic acid, using oleanolic acid as reference, using colorimetric method to test the content at 549nm wavelength. The inventive method can check the triterpenoids content of lucid ganoderma spore oil, with strong reaction specificity, stable method, better repeatability, high sensitivity at 0. 0087 / mug, minimum detect limit as 2mug, and label recycle rate as 98. 15%.

Description

technical field [0001] The invention relates to a method for measuring the content of triterpenoids in ganoderma lucidum spore oil, in particular to a method for indirectly measuring the content of triterpenoids in ganoderma lucidum products by using vanillin as a color developing agent and using a colorimetric method. Background technique [0002] Ganoderma lucidum products include Ganoderma lucidum fruiting body, Ganoderma lucidum mycelium, Ganoderma lucidum spore powder and Ganoderma lucidum spore oil, the active ingredients of which are mainly triterpenes. At present, there are some methods for using vanillin as the color reagent to directly determine the content of triterpenoids in Ganoderma lucidum products by colorimetry, such as the method of Yu Cun et al. (see references: Yu Cun, Yu Sha, Han Jianlong, etc. Ganoderma lucidum Study on the determination method of triterpenoids in health food. Zhejiang Preventive Medicine, 2006, 18(8): 74-75.) Indirect determination of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78G01N21/25
Inventor 徐学平章卫民钟韩陶美华
Owner GUANGDONG INST OF MICROORGANISM
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