The invention relates to a color organic light emitting display panel and a manufacturing method thereof. The manufacturing method is as follows: 1) cleaning and drying bottom substrate glass, sputtering two metal layers on bottom substrate ITO, and annealing by laser; 2) coating photoresist, exposing, developing, cleansing, drying, and etching out an anode pattern; 3) cleaning and drying upper substrate glass, coating photoresist on upper substrate ITO, prebaking, exposing, developing, cleansing, drying, baking, and etching out a metal cathode pattern on upper substrate ITO by etching liquor; 4) spin-coating the bottom substrate glass, baking, exposing, developing, washing, drying, baking again and curing to obtain an insulation column; 5) evaporating various organic pixel layers on the bottom substrate glass; 6) carrying out vacuum aligning gluing on the bottom substrate glass and the upper substrate glass; and 7) carrying out vacuum lamination on a glued display panel, curing by irradiation of an ultraviolet lamp to obtain the color organic light emitting display panel. The method can effectively improve the service life and light-extraction efficiency of a device, reduce loss of light power source and save working procedures.