Electrochemical detection of nucleic acid hybridization

a nucleic acid hybridization and electrochemical detection technology, applied in the field of nucleic acid hybridization and sequencing, can solve problems such as disadvantageous techniques
US20050233358A1Inactive Publication Date: 2005-10-20THORP H +5
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
THORP H
Publication Date
2005-10-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.
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Description

[0001] This application is a continuation-in-part of copending application Ser. No. 08 / 495,817 filed Jun. 27, 1995, and is a continuation-in-part of copending application Ser. No. 60 / 016,265 filed Apr. 19, 1996, the disclosures of which are incorporated by reference herein in their entirety.FIELD OF THE INVENTION

[0002] The present invention relates to nucleic acid hybridization and sequencing, and particularly to methods of qualitatively and quantitatively detecting nucleic acid hybridization and to methods of nucleic acid sequencing. BACKGROUND OF THE INVENTION

[0003] The detection of individual DNA sequences in heterogenous samples of DNA provides a basis for identifying genes, DNA profiling, and novel approaches to DNA sequencing. One approach to DNA hybridization detection involves the use of surface bound DNA sequences which can be assayed using an analytical response that indicates hybridization of the surface-bound oligomer to a sequence in the heterogeneous sample. These an...

Claims

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