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Preparation method for noble metal nanoclusters of fluorescent sensor

A fluorescence sensor and nano-cluster technology, applied in the field of preparation of noble metal nano-clusters, can solve the problems of unfavorable promotion and preparation, time-consuming and high cost, long reaction time, etc., and achieve easy promotion, low energy consumption, and consistent surface morphology. Effect

Inactive Publication Date: 2013-10-23
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many traditional methods require a single protein, and the reaction time is long, requiring heating and adding reducing agents, etc., which are time-consuming and costly, and are not conducive to large-scale promotion and preparation

Method used

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  • Preparation method for noble metal nanoclusters of fluorescent sensor
  • Preparation method for noble metal nanoclusters of fluorescent sensor
  • Preparation method for noble metal nanoclusters of fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, gold nanocluster:

[0037] 1. For the formation conditions of red gold nanoclusters, take 5 ml of egg white (1:2 diluted with water), add 5 ml of HAuCl (10 mM concentration), and mix well; then add 1 ml of sodium hydroxide (1 M), mix Homogenize for 2 minutes; finally add sodium borohydride (or not). After completing the above conditions, nanoclusters with strong fluorescence are formed after standing for 10-12 hours.

[0038] 2. Conditions for the formation of pink gold nanoclusters: take 5 ml egg white (1:2 diluted with water), add 2.5 ml HAuCl (10mM concentration), add 2.5ml H 2 0, mix well; then add 1ml sodium hydroxide (1M), mix well for 2 min; finally add sodium borohydride (or not). After the above-mentioned conditions are completed, nanoclusters with strong fluorescence are formed after being left at room temperature for 10-12 hours.

[0039] 3. Conditions for the formation of blue gold nanoclusters: take 5 ml egg white (1:2 diluted with water), ...

Embodiment 2

[0040] Embodiment 2, platinum nanoclusters:

[0041] Conditions for the formation of blue platinum nanoclusters: take 5 ml egg white (diluted 1:2 with water), add 5 ml HPtCl (concentration: 1 mM), mix well; then add 1 ml sodium hydroxide (1M), mix well 2 min; water bath 55 degrees. After the above-mentioned conditions are completed, nanoclusters with strong fluorescence are formed after being left at room temperature for 10-12 hours.

Embodiment 3

[0042] Embodiment 3, silver nanoclusters:

[0043] 5 ml egg white (1:2 diluted with water), add 5 ml AgNO3 (concentration: 10 mM), mix well; add 1 ml sodium hydroxide (1M), mix well; finally add sodium borohydride (or not) After completing the above conditions, the fluorescent nanoclusters are formed after being left at room temperature for 1-2 hours. But its stability needs to be enhanced.

[0044] figure 1 It is the topography of liquid noble metal nanoclusters under the irradiation of visible light and ultraviolet light (λ=365nm), such as figure 1 As shown above, under the irradiation of visible light, the gold nanoclusters and platinum nanoclusters which vary with the concentration of HAuCl are colorless, light yellow, dark brown and light green, respectively; respectively exhibit strong fluorescence effects.

[0045] figure 2 It is a TEM image of red fluorescent gold nanoclusters. It can be seen from the figure that the size of gold nanoclusters is relatively un...

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Abstract

The invention relates to a preparation method for noble metal nanoclusters of a fluorescent sensor. The preparation method comprises the following steps: sucking egg white from a fresh egg, diluting by purified water, and uniformly mixing; finally fully and uniformly mixing the diluted egg white with chloroauric acid (HAuCl4), uniformly mixing a mixture with sodium hydroxide, and standing to prepare red fluorescent gold nanoclusters. A preparation method for silver and platinum nanoclusters is similar to that for the gold nanoclusters, and silver nitrate and chloroplatinic acid (H2PtCl6) are added into the diluted egg white respectively in the preparation method for the silver and platinum nanoclusters. The clusters prepared by the method are uniform in size, uniform in surface appearance, very strong in fluorescence and ultra-low in detection sensitivity. The method is simple, novel, simple in materials, small in equipment number, low in energy consumption and convenient to popularize, and has a wide application prospect in the fields of disease detection, gas adsorption, chemical catalysis, food safety, photoelectronic devices and the like.

Description

technical field [0001] The invention relates to a method for preparing a noble metal nano-cluster used for a fluorescence sensor, and belongs to the field of material preparation and application. Background technique [0002] Traditional fluorescent markers such as rhodamine, fluorescein, pironine, and phenanthridine dyes generally have disadvantages such as cytotoxic side effects, poor photostability, narrow excitation spectrum, and oxygen sensitivity. Noble metal nanoclusters are fluorescent, water-soluble molecular-level aggregates composed of a few to tens of atoms of noble metals such as Au, Ag, or Pt. It has the characteristics of good biocompatibility, strong photostability, and strong anti-polymerization ability, which greatly improves its performance as a biomarker. At present, noble metal nanoclusters have been gradually applied in the fields of biosensors, cell labeling and imaging, and biological probes. [0003] At present, there are many kinds of synthesis me...

Claims

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Application Information

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IPC IPC(8): B22F9/24
Inventor 张柯张小秋尹桂林何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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