Preparation method for noble metal nanoclusters of fluorescent sensor

A fluorescence sensor and nano-cluster technology, applied in the field of preparation of noble metal nano-clusters, can solve the problems of unfavorable promotion and preparation, time-consuming and high cost, long reaction time, etc., and achieve easy promotion, low energy consumption, and consistent surface morphology. Effect

Inactive Publication Date: 2013-10-23
SHANGHAI NAT ENG RES CENT FORNANOTECH
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Problems solved by technology

Many traditional methods require a single protein, and the reaction time is long, requiring heating and adding reduc

Method used

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  • Preparation method for noble metal nanoclusters of fluorescent sensor
  • Preparation method for noble metal nanoclusters of fluorescent sensor
  • Preparation method for noble metal nanoclusters of fluorescent sensor

Examples

Experimental program
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[0036] Example 1. Gold nanoclusters:

[0037] 1. The conditions for the formation of red gold nanoclusters, take 5 ml of egg white (diluted with 1:2 water), add 5 ml of HAuCl (concentration of 10 mM), and mix; then add 1 ml of sodium hydroxide (1 M), and mix Homogenize for 2 minutes; finally, add sodium borohydride (or not). After completing the above conditions, leave it for 10-12 hours to form nanoclusters with strong fluorescence.

[0038] 2. Conditions for the formation of pink gold nanoclusters: take 5 ml of egg white (diluted with 1:2 water), add 2.5 ml of HAuCl (concentration of 10 mM), and add 2.5 ml of H 2 0, mix well; then add 1ml sodium hydroxide (1M), mix well for 2 min; finally add sodium borohydride (or not). After completing the above-mentioned conditions, leave it at room temperature for 10-12 hours to form nanoclusters with strong fluorescence.

[0039] 3. Conditions for the formation of blue gold nanoclusters: Take 5 ml of egg white (diluted in 1:2 with water), ad...

Example Embodiment

[0040] Example 2. Platinum nanoclusters:

[0041] Conditions for the formation of blue platinum nanoclusters: Take 5 ml of egg white (diluted with 1:2 water), add 5 ml of HPtCl (concentration of 1 mM), and mix; then add 1 ml of sodium hydroxide (1M), and mix well 2 min; water bath 55 degrees. After completing the above-mentioned conditions, leave it at room temperature for 10-12 hours to form nanoclusters with strong fluorescence.

Example Embodiment

[0042] Example 3. Silver nanoclusters:

[0043] 5 ml egg white (diluted with 1:2 water) add 5 ml AgNO3 (concentration of 10 mM), mix well; add 1 ml sodium hydroxide (1M), mix well; finally add sodium borohydride (or not) After completing the above conditions, leave it at room temperature for 1-2 hours to form fluorescent nanoclusters. But its stability needs to be enhanced.

[0044] figure 1 It is the topography of liquid precious metal nanoclusters under visible light and ultraviolet light (λ=365nm), such as figure 1 As shown above, under visible light irradiation, the gold nanoclusters and platinum nanoclusters changing with the concentration of HAuCl are colorless, light yellow, dark brown and light green, respectively; while under ultraviolet light (λ=365nm) Each shows a strong fluorescence effect.

[0045] figure 2 It is a TEM image of red fluorescent gold nanoclusters. It can be seen from the figure that the size of the gold nanoclusters is relatively uniform and the sur...

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Abstract

The invention relates to a preparation method for noble metal nanoclusters of a fluorescent sensor. The preparation method comprises the following steps: sucking egg white from a fresh egg, diluting by purified water, and uniformly mixing; finally fully and uniformly mixing the diluted egg white with chloroauric acid (HAuCl4), uniformly mixing a mixture with sodium hydroxide, and standing to prepare red fluorescent gold nanoclusters. A preparation method for silver and platinum nanoclusters is similar to that for the gold nanoclusters, and silver nitrate and chloroplatinic acid (H2PtCl6) are added into the diluted egg white respectively in the preparation method for the silver and platinum nanoclusters. The clusters prepared by the method are uniform in size, uniform in surface appearance, very strong in fluorescence and ultra-low in detection sensitivity. The method is simple, novel, simple in materials, small in equipment number, low in energy consumption and convenient to popularize, and has a wide application prospect in the fields of disease detection, gas adsorption, chemical catalysis, food safety, photoelectronic devices and the like.

Description

technical field [0001] The invention relates to a method for preparing a noble metal nano-cluster used for a fluorescence sensor, and belongs to the field of material preparation and application. Background technique [0002] Traditional fluorescent markers such as rhodamine, fluorescein, pironine, and phenanthridine dyes generally have disadvantages such as cytotoxic side effects, poor photostability, narrow excitation spectrum, and oxygen sensitivity. Noble metal nanoclusters are fluorescent, water-soluble molecular-level aggregates composed of a few to tens of atoms of noble metals such as Au, Ag, or Pt. It has the characteristics of good biocompatibility, strong photostability, and strong anti-polymerization ability, which greatly improves its performance as a biomarker. At present, noble metal nanoclusters have been gradually applied in the fields of biosensors, cell labeling and imaging, and biological probes. [0003] At present, there are many kinds of synthesis me...

Claims

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Application Information

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IPC IPC(8): B22F9/24
Inventor 张柯张小秋尹桂林何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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