The present invention belongs to the data acquisition field, in particular to a multifunctional data acquisition card and a measurement method. The multifunctional data acquisition card provided by the present invention comprises a communication interface circuit (1), a microcontroller (2), an analog-digital converter (3), a programmable amplifier (4), a measurement type switching module (5) and an input selection relay module (6). The multifunctional data acquisition card of the present invention acquires the types of the data, such as the voltages, the currents, the thermal resistance, the thermocouple, the resistance bridge type pressure sensor signals, etc., by switching the electronic switches, and the microcontroller is set to carry out the logic control, so that various types of data is acquired on a single data acquisition card, and the number of the devices applied by a related measurement system and the cost are reduced. According to the present invention, a relay is arrangedon the data acquisition card and is used to select whether a currently accessed measurement signal is a to-be-measured signal or a calibration signal, thereby realizing an online calibration function.