High-yield microalgae cultivating method

A microalgae culture and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of slow cell growth, low yield of target products, and long cultivation period of algae species, achieving The effect of high productivity, small footprint, and reduction in the number of devices and equipment

Active Publication Date: 2011-08-17
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopting the method of the present invention can give full play to the advantages of the rapid growth of microalgae in the heterotrophic stage, not only can provide a large number of algae species in time for the large-scale photoautotrophic cultivation of microalgae, but also can accelerate the photoautotrophic growth of microalgae and the target product ( Such as oil, protein, etc.) formation rate provides an important technical means to solve the problems of long expansion period of algae species, slow cell growth and low yield of target products in the process of large-scale photoautotrophic culture of microalgae

Method used

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Examples

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Effect test

Embodiment 1

[0087] Example 1: Research on Algae Cell Growth During Heterotrophic and Photoautotrophic Culture of Chlorella pyrenoidosa Seeds

[0088] The Chlorella pyrenoidosa of this example was cultured heterotrophically in a 500ml shake flask and cultured photoautotrophically in a 2L Erlenmeyer flask. As the seeds of photoautotrophic culture in the next step, the algae cell growth curves of heterotrophic and photoautotrophic cultured seeds of Chlorella pyrenoidosa were measured respectively. The inoculation density of Chlorella pyrenoidosa seeds in heterotrophic culture is 0.20g / l, the temperature is 30°C, and the rotation speed is 150rmp. After 72 hours of cultivation, the glucose in the culture medium has been exhausted, and the algae cell density is 6.8g / l , for the seeds of the next photoautotrophic culture; the inoculation density of Chlorella pyrenoidosa seeds during photoautotrophic culture is 0.20g / l, the temperature is 25°C, the light intensity is 2000lx, and the light-dark cy...

Embodiment 2

[0089] Example 2: Research on Algae Cell Growth During Heterotrophic and Photoautotrophic Cultivation of Chlorella vulgaris Seeds

[0090] The Chlorella vulgaris of this embodiment is respectively carried out heterotrophic culture in a 500ml shake flask and photoautotrophic culture in a 2L Erlenmeyer flask. Growth curves of algal cells and photoautotrophic cultured seeds. The inoculation density of Chlorella vulgaris was 0.30g / l during heterotrophic culture, the temperature was 30°C, and the rotation speed was 150rmp. After 72 hours of cultivation, the glucose in the culture medium was exhausted, and the algae cell density was 8.1g / l. Seeds for the next step of photoautotrophic cultivation; the inoculation density of Chlorella vulgaris seeds during photoautotrophic cultivation is 0.30g / l, the temperature is 25°C, the light intensity is 2000lx, the light-dark cycle is 24:0, and the time is set every day Shake 3 times, when cultivating to 120h, algal cell is in exponential grow...

Embodiment 3

[0091] Example 3: Research on Algae Cell Growth During Heterotrophic and Photoautotrophic Culture of Chlorella ellipsoida Seeds

[0092] The Chlorella ellipsoides of this embodiment were respectively carried out heterotrophic culture in a 500ml shake flask and photoautotrophic culture in a 2L Erlenmeyer flask, and as the seeds of the next photoautotrophic culture, the heterotrophic cultures of Chlorella ellipsoids were respectively measured. Growth curves of algal cells and photoautotrophic cultured seeds. The inoculation density of Chlorella ellipsoida seeds in heterotrophic culture is 0.25g / l, the temperature is 30°C, and the rotation speed is 150rmp. After 72 hours of cultivation, the glucose in the culture medium has been exhausted, and the algae cell density is 8.8g / l. Seeds used for the next step of photoautotrophic culture; the inoculation density of Chlorella ellipsoida seeds during photoautotrophic culture is 0.25g / l, the temperature is 25°C, the light intensity is 20...

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Abstract

The invention relates to a high-yield microalgae cultivating method. The method comprises a step of carrying out heterotrophic cultivation on microalgae algae species and a step of carrying out photoautotrophic cultivation by using algae cells obtained by heterotrophic cultivation as seeds. For the microalgae capable of growing heterotrophically, due to the adoption of the method, the advantage that the algae cells grow rapidly in the heterotrophic phase of the microalgae can be sufficiently played, not only a great amount of algae species can be provided in time for large scale photoautotrophic cultivation of the microalgae, but also the photoautotrophic growth of the microalgae and a forming rate of target products (such as oil, proteins and the like) can be quickened. An important technical means is provided for solving the problems of long algae specie spreading cultivation period, low cell growing speed and low yield of the target products in the large scale photoautotrophic cultivation process of the microalgae.

Description

technical field [0001] The invention belongs to the field of bioenergy and / or the field of microalgae biotechnology, and relates to a high-yield microalgae cultivation method. Background technique [0002] Microalgae cells are rich in various high-value active substances such as protein, polysaccharide, fatty acid and carotenoid. Therefore, microalgae are widely used in many aspects such as food, feed, medicine, environmental protection and bioenergy. [0003] The main culture methods of microalgae are photoautotrophic, mixed nutrition and heterotrophic culture. At present, the microalgae (such as Chlorella, Spirulina, Salina, Haematococcus pluvialis, etc.) that have been industrialized are almost all cultured by photoautotrophy. [0004] The mixed nutrient culture of microalgae needs to be cultivated in a sterilizable photobioreactor, which needs to ensure aseptic culture and sufficient light conditions at the same time. The requirements for the cultivation equipment are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12P21/00C12R1/89
CPCC12P7/6463C12N1/12C12P7/64C12P21/00
Inventor 李元广韩菲菲王伟良黄建科梁松涛章真严逸陈铖王军范建华李淑兰沈国敏
Owner EAST CHINA UNIV OF SCI & TECH
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