Fluorescent carbon quantum dot and preparation method and application thereof

A technology of carbon quantum dots and fluorescence, applied in the field of fluorescent carbon quantum and its preparation, can solve the problems of cumbersome detection methods, complex data analysis, expensive equipment, etc., and achieve good optical stability, high fluorescence quantum yield, good biological compatibility effect

Active Publication Date: 2018-11-23
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the detection of nucleic acid requires expensive and precise instruments and takes a lot of time, and some detection methods are cumbersome and require professional operators, this greatly hinders the large-scale promotion and application of the market
[0004] Microscopic observation (including atomic force microscopy, electron microscopy, and fluorescence microscopy) and spectroscopic detection (including Raman spectroscopy and infrared spec

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  • Fluorescent carbon quantum dot and preparation method and application thereof
  • Fluorescent carbon quantum dot and preparation method and application thereof
  • Fluorescent carbon quantum dot and preparation method and application thereof

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Embodiment 1

[0052] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0053] (1) Weigh 0.1g of 1,4-naphthalene dicarboxylic acid, 0.2g of urea and dissolve it in 40mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0054] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0055] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 1000 Da ...

Embodiment 2

[0073] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0074] (1) Weigh 0.20g of 1,4-naphthalene dicarboxylic acid and 0.20g of urea and dissolve it in 39mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0075] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0076] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 100...

Embodiment 3

[0078] This embodiment provides a method for preparing fluorescent carbon quantum, which specifically includes the following steps:

[0079] (1) Weigh 0.20g of 1,4-naphthalene dicarboxylic acid, 0.10g of urea and dissolve it in 40.4mL of 85wt% phosphoric acid solution (density: 1.689g / mL), stir well to obtain the precursor solution;

[0080] (2) Transfer the precursor solution to an 80mL Teflon-lined stainless steel reactor, tighten the lid, and react at a temperature of 200°C for 9 hours; then let the reactor naturally cool to room temperature to obtain a fluorescent Aqueous solution of carbon quantum dots;

[0081] (3) Add NaOH solution to the aqueous solution containing fluorescent carbon quantum dots cooled to room temperature and stir to adjust the pH of the aqueous solution containing fluorescent carbon quantum dots to 7.4. Then the aqueous solution containing the fluorescent carbon quantum dots was transferred into a dialysis bag with a molecular weight cut-off of 1000...

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Abstract

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is a nitrogen and phosphor-doped carbon quantum dot; a carbon source of the fluorescent carbon quantum dotis naphthalenedicarboxylic acid; the fluorescent carbon quantum dot has high yield rate of fluorescent quantum, can enter the microorganism to specially mark after the microorganism is deactivated, and the death and living of the microorganism can be effectively distinguished. The invention also discloses a preparation method of the fluorescent carbon quantum dot. The preparation method has the advantages that the raw materials are easy to obtain, and the preparation method is suitable for preparing the fluorescent carbon quantum dot with high yield rate of quantum. The invention discloses application of the fluorescent carbon quantum dot in activity detection of microorganisms and biomedical imaging; the fluorescent carbon quantum dot is used for effectively distinguishing the death and living of the microorganisms, so as to detect the activity of the microorganism at high efficiency; the cell toxicity of the fluorescent carbon quantum dot is low, and the fluorescent carbon quantum dot is suitable for in-vitro or in-vivo biomedical imaging detection.

Description

technical field [0001] The invention belongs to the technical field of carbon nanomaterials, and in particular relates to a fluorescent carbon quantum and its preparation method and application. Background technique [0002] Medical care and sanitation are important areas related to people's livelihood in my country. Among them, microbial activity is an important indicator for evaluating disease infection, environmental pollution, food safety, and antibacterial drug efficacy. In the pathogenicity detection of water, food, and clinical samples, antibacterial It has played an important role in drug development, disease prevention and control. Therefore, it is of great significance to develop simple, efficient and accurate detection methods for microbial activity. [0003] The traditional microbial activity detection mostly adopts the plate colony counting method, which is easy to operate and the detection results are reliable. However, the counting detection can only be carrie...

Claims

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Application Information

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IPC IPC(8): C09K11/70C09K11/06B82Y20/00B82Y30/00B82Y40/00G01N21/64A61K49/00
CPCA61K49/0067B82Y20/00B82Y30/00B82Y40/00C09K11/06C09K11/70G01N21/6428
Inventor 董文飞
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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