38results about How to "The detection signal is obvious" patented technology

The invention discloses a magnetostrictive displacement sensor suitable for a large temperature range / high temperature environment. The sensor comprises a pulse generating circuit, a constant elasticdevice, a detecting coil, a control sampling module, a magnetostrictive waveguide wire, a casing, a thermocouple, a waveguide wire insulating sleeve and a material level mark. A left side of the casing is a component bin, a right side of the casing is a hollow pipe, and the component bin and the hollow pine communicate with each other and have the same center axis. The magnetostrictive waveguide wire is Fe-Ga or Fe-Co material. The waveguide wire is fixed in the sensor casing. The detection coil sleeves a left side of the waveguide wire. The material level mark sleeves a right end of the casing. A magnetic material of the sensor obtained by the invention has a higher Curie temperature, and the novel displacement sensor can be applied to a high-temperature environment below 500 DEG C.

The invention provides a 7-diethylamino-3-acetyl coumarin derivative and its synthesis method and application, the derivative is 4-(3-(7-(diethylamino)-2- Oxo-2H-chromium-3-yl)-5-(p-tolyl)-4,5-dihydro-1H-pyrazole-1-yl)benzoic acid, the English name is 4-(3-(7- (diethylamino)‑2‑oxo‑2H‑chromen‑3‑yl)‑5‑(p‑tolyl)‑4,5‑dihydro‑1H‑pyrazol‑1‑yl) benzoic acid, named Probe 1; or 4 ‑(3‑(7‑(diethylamino)‑4‑hydroxy‑2‑oxo‑2H‑chromium‑3‑yl)‑5‑(p-tolyl)‑4,5‑dihydro‑1H‑pyrazole ‑1‑yl)benzoic acid, the English name is 4‑(3‑(7‑(diethylamino)‑4‑hydroxy‑2‑oxo‑2H‑chromen‑3‑yl)‑5‑(p‑tolyl)‑4, 5‑dihydro‑1H‑pyrazol‑1‑y l)benzoic acid, named Probe 2. In PBS (pH=7.4) buffer solution, Probe 1 and ClO ‑ No reaction, Probe 2 can realize "turn-off" detection of ClO ‑ . The detection process is simple, sensitive and fast, and the detection result is accurate.

The invention discloses a detector for the coagulation function of whole blood. The detector comprises a detecting vessel, a rotating device, a light wave generator, a light wave receiver, a timer and a shell, wherein the detecting vessel is used for placing the whole blood; the rotating device, the light wave generator, the light wave receiver, the timer and the shell are connected with the detecting vessel; the rotating device is used for driving the detecting vessel to rotate around the self central axis; the light wave generator and the light wave receiver are oppositely arranged on the two sides of the detecting vessel; when the whole blood inside the detecting vessel is not coagulated, the light wave receiver always receives the quite strong light emitted by the light wave generator continuously, cannot or can only receive the weak light signal emitted by the light wave generator in the rotation process of the detecting vessel; and the timer is used for recording the time that the light wave receiver, into which a coagulation reagent is added, cannot receive the light emitted by the light wave generator for the first time or cannot stably receive the light emitted by the light wave generator, or the time that the light wave receiver receives the quite strong light signal for the first time. The invention also discloses a method for detecting the coagulation function of the whole blood.

The invention provides a specific response ONOO based on resorufin dye ‒ Fluorescent probe, preparation method and application, the structural formula of the probe is:. In this probe, resorufin dye is selected as the fluorophore, and diphenylphosphinyl ester is used as the recognition group. The detection mechanism is ONOO ‒ Oxidation reaction with diphenylphosphinyl ester group, followed by one-step self-elimination reaction, releases fluorophore to cause fluorescence signal change. The above probes detect ONOO by UV and fluorescence spectrometers ‒ And without the interference of reactive nitrogen and reactive oxygen species, the detection process is simple, rapid and sensitive, with a detection limit of 238 nM. More importantly, the probe can detect ONOO in cells and in vivo ‒ , has a good application prospect in the field of biological monitoring.

The invention provides a fluorescent probe for distinguishing and detecting Cys / Hcy and GSH, its preparation method and application. The Chinese name of the fluorescent probe is 10‑(diethylamino)‑3‑((7‑nitrobenzo[c][1,2,5]oxadiazol‑4‑yl)oxy)‑ 5,6‑Dihydrobenzo[c]xanthene12ium, named NBD‑O‑1. The invention provides a method for the detection of Cys / Hcy and GSH by fluorescent probe NBD-O-1, which is prepared in PBS (pH 7.4, containing 20% ​​CH 3 CN, v / v) solution, the qualitative distinction of Cys / Hcy and GSH by ultraviolet and fluorescence spectrometers is not interfered by common amino acids. More importantly, the probe can be applied to the detection of Cys / Hcy and GSH in animals and plants, and the detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention provides a reagent for distinguishing and detecting thiophenol and its synthesis method and application. The reagent is a benzothiazole derivative: the Chinese name of the derivative is (E)‑2‑(benzo Thiazol-2-yl)-6-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)-4-methyl Phenyl 2,4-dinitrobenzenesulfonate, the English name is (E)-2-(benzothiazol-2-yl)-6-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1 ‑en‑1‑yl)vinyl)‑4‑me thylphenyl 2,4‑dinitrobenzenesulfonate, named SYN. The detection method is Hepes‑CH at pH 7.4 3 OH‑CH 3 In the buffer solution of CN (1:1:1, v / v / v), the content of thiophenol was quantitatively detected by fluorescence spectrophotometer. The detection process is simple, sensitive and fast, and the detection result is accurate.

The invention provides an isoxazole derivative as well as a synthesis method and application thereof to detection of hydrogen sulfide. The Chinese name of the isoxazole derivative is (E)-5-(2-(5,5-difluoro-10-(4-methoxyphenyl)-1,3,7,9tetramethyl-5hydrodipyrrolo[1,3,2]diazaboro-2-yl)vinyl)-3-methyl-4-nitroisoxazole. The invention also provides a method for rapidly detecting the hydrogen sulfide; based on the isoxazole derivative, the method is used for quantitatively detecting the content of the hydrogen sulfide in a PBS (Phosphate Buffer Solution) (with the pH (Potential of Hydrogen) of 7.4) through a fluorescence spectrometer. By adopting the method, the hydrogen sulfide is rapidly and conveniently detected.