38results about How to "The detection signal is obvious" patented technology

The invention provides a benzothiazole derivative and a synthesis method and application thereof. The Chinese name of the derivative is called as (E)-2-(3-(benzo[d]thiazole-2-yl)-2-hydroxy-5-methylstyryl)-3-methylbenzo[d]thiazole-3-iodide, and the structural formula of the derivative is shown in the specification. The English name of the compound is (E)-2-(3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methlyryl)-3-methlylbenzo[d]thiazol-3-iumiodide, and the structural formula of the compound is shown in the specification. The reagent is named as NSSN, and can realize dual-mode detection of hypochlorousacid. According to the detection method, in a buffer solution of PBS / CH3CN (1: 1, v / v, pH=7.4), the content of hypochlorous acid is detected through a fluorescence spectrophotometer ratio type and aturn-on type. The detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention discloses a magnetostrictive displacement sensor suitable for a large temperature range / high temperature environment. The sensor comprises a pulse generating circuit, a constant elasticdevice, a detecting coil, a control sampling module, a magnetostrictive waveguide wire, a casing, a thermocouple, a waveguide wire insulating sleeve and a material level mark. A left side of the casing is a component bin, a right side of the casing is a hollow pipe, and the component bin and the hollow pine communicate with each other and have the same center axis. The magnetostrictive waveguide wire is Fe-Ga or Fe-Co material. The waveguide wire is fixed in the sensor casing. The detection coil sleeves a left side of the waveguide wire. The material level mark sleeves a right end of the casing. A magnetic material of the sensor obtained by the invention has a higher Curie temperature, and the novel displacement sensor can be applied to a high-temperature environment below 500 DEG C.

The invention provides a fluorescent probe for selectively responding to H2S based on BODIPY fluorescent dye targeting lysosome, and preparation and application of the probe. The probe selects BODIPY dye as a fluorophore and 2, 4-dinitrobenzenesulfonyl as a recognition group. A sensing mechanism is based on H2S to induce cracking of 2, 4-dinitrobenzenesulfonyl in the probe, so that a photoinduced electron transfer (PET) process is inhibited. The probe detects H2S through ultraviolet and fluorescence spectrometers and is not interfered by other amino acids, active sulfides, representative anions and the like, the detection process is simple, convenient, rapid and sensitive, and the detection limit is 4.75 nM. More importantly, the probe can target lysosome and detect H2S in cells at the same time, and has a good application prospect in the field of biological monitoring.

The invention provides a fluorescent probe based on phenyl selenide group specific response Cys, and a preparation method and application thereof. According to the probe, BODIPY dye is selected as a fluorophore, and diphenyl selenide is selected as a recognition group. The detection mechanism is that Cys and diphenyl selenide are subjected to nucleophilic substitution-rearrangement reaction to cause fluorescence signal change. The probe detects Cys through ultraviolet and fluorescence spectrometers and is not interfered by other amino acids and active oxygen, the detection process is simple, convenient, rapid and sensitive, and the detection limit is 33 nM. More importantly, the probe can be used for detecting Cys in cells and has a good application prospect in the field of biological monitoring.

The invention discloses a magnetostrictive displacement sensor suitable for a large temperature range / high temperature environment. The sensor comprises a pulse generating circuit, a constant elasticdevice, a detecting coil, a control sampling module, a magnetostrictive waveguide wire, a casing, a thermocouple, a waveguide wire insulating sleeve and a material level mark. A left side of the casing is a component bin, a right side of the casing is a hollow pipe, and the component bin and the hollow pine communicate with each other and have the same center axis. The magnetostrictive waveguide wire is Fe-Ga or Fe-Co material. The waveguide wire is fixed in the sensor casing. The detection coil sleeves a left side of the waveguide wire. The material level mark sleeves a right end of the casing. A magnetic material of the sensor obtained by the invention has a higher Curie temperature, and the novel displacement sensor can be applied to a high-temperature environment below 500 DEG C.

The invention provides a method for detecting lime substances in flour with a near-infrared micro-imaging technology. The method comprises the steps of: collecting a near-infrared micro-image of a flour sample; according to the near infrared spectrum characteristics of lime substances and pure flour, subjecting the near-infrared micro-image of the flour sample to an imaging analysis. Without any complex pretreatment in the whole process, the detection of the invention has simple, rapid and pollution-free steps. Compared with traditional methods, the method provided in the invention has more obvious additive detection signals, thus being more conducive to the detection of very small amounts of additives. As near-infrared lights are stronger than mid-infrared lights in terms of penetrability in substances, so they can adapt to rough surfaces. Besides, near-infrared lights not only can collect the information of the sample surface, but also can go deep into the sample to collect information. Therefore, the near-infrared micro-imaging technology is very suitable for detection of lime substances in flour, thus providing a new method for rapid quality safety screening of market flour.

The invention provides a 7-diethylamino-3-acetyl coumarin derivative and its synthesis method and application, the derivative is 4-(3-(7-(diethylamino)-2- Oxo-2H-chromium-3-yl)-5-(p-tolyl)-4,5-dihydro-1H-pyrazole-1-yl)benzoic acid, the English name is 4-(3-(7- (diethylamino)‑2‑oxo‑2H‑chromen‑3‑yl)‑5‑(p‑tolyl)‑4,5‑dihydro‑1H‑pyrazol‑1‑yl) benzoic acid, named Probe 1; or 4 ‑(3‑(7‑(diethylamino)‑4‑hydroxy‑2‑oxo‑2H‑chromium‑3‑yl)‑5‑(p-tolyl)‑4,5‑dihydro‑1H‑pyrazole ‑1‑yl)benzoic acid, the English name is 4‑(3‑(7‑(diethylamino)‑4‑hydroxy‑2‑oxo‑2H‑chromen‑3‑yl)‑5‑(p‑tolyl)‑4, 5‑dihydro‑1H‑pyrazol‑1‑y l)benzoic acid, named Probe 2. In PBS (pH=7.4) buffer solution, Probe 1 and ClO ‑ No reaction, Probe 2 can realize "turn-off" detection of ClO ‑ . The detection process is simple, sensitive and fast, and the detection result is accurate.

The invention provides a triphenylamine-benzopyran onium salt derivative NIR-BT-P as well as a synthesis method and application thereof. The derivative NIR-BT-P has a Chinese name of (E)-6-(diethylamino)-4-(4 (diphenylamino) benzylidene)-1, 2, 3, 4 tetraoxypyrimidine, and the derivative NIR-BT-P has a structural formula shown in the description. The invention relates to a preparation method of a diethylene glycol dimer, which is characterized in that the diethylene glycol dimer has an English name of (E)-6-(diethylene glycol dimer)-4-(4 (diethylene glycol dimer)-1, 2, 3, 4 tetraethylene glycol dimer, and the English name of the diethylene glycol dimer is (E)-6-(diethylene glycol dimer)-4 (4)-1, 2, 3, 4 tetraethylene glycol dimer. The derivative NIR-BT-P is used as a fluorescent probe, and in different organic solvents, detection of polarity by different fluorescence intensity signals is realized through a fluorospectro photometer. According to the method, the difference of polarities in cancer cells and normal cells in a living body is utilized, so that the near-infrared fluorescence imaging is used for distinguishing and detecting tumor tissues and normal tissues in the living body.

The invention discloses a detector for the coagulation function of whole blood. The detector comprises a detecting vessel, a rotating device, a light wave generator, a light wave receiver, a timer and a shell, wherein the detecting vessel is used for placing the whole blood; the rotating device, the light wave generator, the light wave receiver, the timer and the shell are connected with the detecting vessel; the rotating device is used for driving the detecting vessel to rotate around the self central axis; the light wave generator and the light wave receiver are oppositely arranged on the two sides of the detecting vessel; when the whole blood inside the detecting vessel is not coagulated, the light wave receiver always receives the quite strong light emitted by the light wave generator continuously, cannot or can only receive the weak light signal emitted by the light wave generator in the rotation process of the detecting vessel; and the timer is used for recording the time that the light wave receiver, into which a coagulation reagent is added, cannot receive the light emitted by the light wave generator for the first time or cannot stably receive the light emitted by the light wave generator, or the time that the light wave receiver receives the quite strong light signal for the first time. The invention also discloses a method for detecting the coagulation function of the whole blood.

The invention discloses a mercury ion detection reagent and a detection method. The reagent comprises nano sol, nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral signal and carrying out data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface nucleic acid adaptors are changed, i.e. nano particle aggregation in the silver sol and surface enhanced Roman scattering signals of signal molecules are enhanced. In the invention, the SERS (Surface Enhanced Raman Scattering) technology is adopted, the detection limit to the Hg<2+> is at the nmol / L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and high selectivity and is suitable for the quick detection of trace Hg<2+> in environmental water.

The invention relates to an optical device, in particular to an optical polarizer and a method for adjusting its asymmetric transmission characteristics. The optical polarizer includes a base layer and a planar structure, and the planar structure is composed of a plurality of nano-period units with the same structure Rectangular periodic arrays are connected, and each periodic unit contains a structural unit. The structural unit is composed of a rectangular hole and a semicircular hole. The arc of the semicircular hole is separated from or tangent to the long side of the rectangular hole; The diameter of the circular hole is parallel to the long side of the rectangular hole; each nano-period unit is made of noble metal material, and the semicircular hole is completely filled with metal Mg. The structure of the optical polarizer of the present invention can achieve the purpose of adjusting its asymmetric transmission characteristics through the transformation of the properties of the metal filled in the semicircular hole. The adjustment method is simple and convenient, and provides a new idea for the design of the optical polarizer.

The invention provides a specific response ONOO based on resorufin dye ‒ Fluorescent probe, preparation method and application, the structural formula of the probe is:. In this probe, resorufin dye is selected as the fluorophore, and diphenylphosphinyl ester is used as the recognition group. The detection mechanism is ONOO ‒ Oxidation reaction with diphenylphosphinyl ester group, followed by one-step self-elimination reaction, releases fluorophore to cause fluorescence signal change. The above probes detect ONOO by UV and fluorescence spectrometers ‒ And without the interference of reactive nitrogen and reactive oxygen species, the detection process is simple, rapid and sensitive, with a detection limit of 238 nM. More importantly, the probe can detect ONOO in cells and in vivo ‒ , has a good application prospect in the field of biological monitoring.

The invention provides a fluorescent probe for distinguishing and detecting Cys / Hcy and GSH, its preparation method and application. The Chinese name of the fluorescent probe is 10‑(diethylamino)‑3‑((7‑nitrobenzo[c][1,2,5]oxadiazol‑4‑yl)oxy)‑ 5,6‑Dihydrobenzo[c]xanthene12ium, named NBD‑O‑1. The invention provides a method for the detection of Cys / Hcy and GSH by fluorescent probe NBD-O-1, which is prepared in PBS (pH 7.4, containing 20% ​​CH 3 CN, v / v) solution, the qualitative distinction of Cys / Hcy and GSH by ultraviolet and fluorescence spectrometers is not interfered by common amino acids. More importantly, the probe can be applied to the detection of Cys / Hcy and GSH in animals and plants, and the detection process is simple, sensitive and rapid, and the detection result is accurate.

The invention provides a reagent for distinguishing and detecting thiophenol and its synthesis method and application. The reagent is a benzothiazole derivative: the Chinese name of the derivative is (E)‑2‑(benzo Thiazol-2-yl)-6-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)-4-methyl Phenyl 2,4-dinitrobenzenesulfonate, the English name is (E)-2-(benzothiazol-2-yl)-6-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1 ‑en‑1‑yl)vinyl)‑4‑me thylphenyl 2,4‑dinitrobenzenesulfonate, named SYN. The detection method is Hepes‑CH at pH 7.4 3 OH‑CH 3 In the buffer solution of CN (1:1:1, v / v / v), the content of thiophenol was quantitatively detected by fluorescence spectrophotometer. The detection process is simple, sensitive and fast, and the detection result is accurate.

The invention relates to a magnetostrictive micro-displacement meter, which is characterized in that the displacement meter includes a displacement meter end, a displacement meter base, a measuring rod, a waveguide wire, a linear guide rail and a movable terminal, and the displacement meter end is a cavity structure , a hardware circuit system is installed on the inner wall of the cavity structure, and a front damping device, a detection device and a fixing device are arranged in the cavity of the cavity structure; the right bottom of the end of the displacement meter is connected with the base of the displacement meter; The center of the right side of the end is provided with a threaded hole, and the left end of the measuring rod is threadedly connected with the threaded hole at the end of the displacement meter; the movable terminal is set on the measuring rod, and the linear guide rail is fixed on the base of the displacement meter along the length direction. A slider is installed on the top, the upper end of the slider is connected with the lower end of the movable terminal, and the slider is connected with an external mechanical device; the magnet inside the movable terminal adopts a "halbach" magnet array.

The invention provides a BODIPY-based fluorescent dye targeting lysosomes selectively responding to H 2 The fluorescent probe, preparation and application of S, the probe uses BODIPY dye as the fluorophore, and 2,4-dinitrobenzenesulfonyl as the recognition group. The sensing mechanism is based on the H 2 S induces the cleavage of the 2,4‑dinitrobenzenesulfonyl group in the probe, thereby inhibiting the process of photoinduced electron transfer (PET), resulting in the restoration of probe fluorescence. The above probes were detected by UV and fluorescence spectrometers for H 2 S is free from interference from other amino acids, active sulfides, and representative anions. The detection process is simple, rapid, and sensitive, with a detection limit of 4.75 nM. More importantly, the probe can target lysosomes and simultaneously detect H in cells 2 S, has a good application prospect in the field of biological monitoring.

The invention provides an isoxazole derivative as well as a synthesis method and application thereof to detection of hydrogen sulfide. The Chinese name of the isoxazole derivative is (E)-5-(2-(5,5-difluoro-10-(4-methoxyphenyl)-1,3,7,9tetramethyl-5hydrodipyrrolo[1,3,2]diazaboro-2-yl)vinyl)-3-methyl-4-nitroisoxazole. The invention also provides a method for rapidly detecting the hydrogen sulfide; based on the isoxazole derivative, the method is used for quantitatively detecting the content of the hydrogen sulfide in a PBS (Phosphate Buffer Solution) (with the pH (Potential of Hydrogen) of 7.4) through a fluorescence spectrometer. By adopting the method, the hydrogen sulfide is rapidly and conveniently detected.