Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms
A technology for quantitative analysis of aquatic organisms, applied in the field of quantitative detection of microplastic particles based on fluorescent tracer technology, can solve the problems of inaccurate analysis of MPs enrichment and distribution, and achieve effective quantitative detection, simple equipment, and high sensitivity of the method Effect
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[0038] The experiment for quantitatively analyzing the enrichment and distribution of microplastics in aquatic organisms includes the following steps.
[0039] (1) Exposure experiment: Fluorescently labeled microplastic polystyrene particles (MPs-PS) were used as the test object, and the test animals were mature and healthy zebrafish ( Daniorerio , 5 months old, 0.29±0.02g), were randomly divided into the exposure group and the control group, each group contained 3 parallels, each parallel used 5 fish, and the exposure period was 7 days. The control group used UV-sterilized aerated tap water, and the exposure group used 5 μm and 20 μm fluorescent MPs-PS (5 μm-MPs-PS, 20 μm-MPs-PS, excitation wavelength 418 nm, emission wavelength 518 nm). The 20mg / LMPs-PS system was prepared by ultraviolet disinfection and aeration of tap water. Other experimental conditions are as follows: temperature: 24±1℃, pH: 7.2±0.5, dissolved oxygen: 6.6±0.3mg / L, conductivity: 0.256±0.005mS / cm, hardne...
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