Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms

A technology for quantitative analysis of aquatic organisms, applied in the field of quantitative detection of microplastic particles based on fluorescent tracer technology, can solve the problems of inaccurate analysis of MPs enrichment and distribution, and achieve effective quantitative detection, simple equipment, and high sensitivity of the method Effect

Active Publication Date: 2016-06-08
NANJING UNIV
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Problems solved by technology

[0005] Aiming at the problem that existing methods cannot accurately analyze the enrichment and distribution of MPs in aquatic organisms, the present invention provides a method for quantitat

Method used

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  • Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms
  • Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms
  • Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms

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Embodiment

[0038] The experiment for quantitatively analyzing the enrichment and distribution of microplastics in aquatic organisms includes the following steps.

[0039] (1) Exposure experiment: Fluorescently labeled microplastic polystyrene particles (MPs-PS) were used as the test object, and the test animals were mature and healthy zebrafish ( Daniorerio , 5 months old, 0.29±0.02g), were randomly divided into the exposure group and the control group, each group contained 3 parallels, each parallel used 5 fish, and the exposure period was 7 days. The control group used UV-sterilized aerated tap water, and the exposure group used 5 μm and 20 μm fluorescent MPs-PS (5 μm-MPs-PS, 20 μm-MPs-PS, excitation wavelength 418 nm, emission wavelength 518 nm). The 20mg / LMPs-PS system was prepared by ultraviolet disinfection and aeration of tap water. Other experimental conditions are as follows: temperature: 24±1℃, pH: 7.2±0.5, dissolved oxygen: 6.6±0.3mg / L, conductivity: 0.256±0.005mS / cm, hardne...

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Abstract

The invention discloses a method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms and belongs to the field of environment pollutant detection. The method is a method for quantitatively analyzing micro plastics in aquatic organisms based on the fluorescence tracer technology. The method includes the steps that tested fluorescent micro plastics and mode aquatic organisms are selected for poisoning experiments; target tissue samples are acquired; tissue is decomposed through nitric acid, and the volume of obtained decomposition liquid is made constant; fluorescent micro plastics turbid solutions in different concentration gradients are prepared, and a standard curve is obtained through fluorescence values measured by a fluorescence spectrometer; the fluorescent value of a sample solution is measured, and the content of micro plastics in the corresponding tissue samples is obtained based on the standard curve. By means of the technical scheme, micro plastics ingested in aquatic organisms can be accurately and quantitatively measured, and the purpose of detecting enrichment and distribution of micro plastics in aquatic organisms is achieved.

Description

technical field [0001] The invention belongs to the field of environmental pollutant detection, and specifically relates to a method for quantitatively analyzing the enrichment and distribution of microplastics in aquatic organisms, and is a quantitative detection method for microplastic particles based on fluorescence tracing technology. Background technique [0002] Plastics are widely used because of their high stability, durability, and corrosion resistance, while a large amount of plastic use and discharge have brought increasing pollution to the environment, especially small-sized plastic waste. Microplastics (MPs) refer to plastic particles, fragments, fibers, etc. with a particle size of less than 5 μm, which are derived from industrial raw plastic materials, personal care products, or degraded from large-size plastics. Invertebrates (crustaceans, bivalve molluscs, polychaetes), vertebrates (seabirds, marine fish) and even mammals (whales) can consume MPs, and MPs ca...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486G01N2021/6417
Inventor 张宴陆熠峰邓永锋任洪强
Owner NANJING UNIV
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