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Medical hydrogel with pH sensitivity, self-healing nature and cell adhesion and preparation method thereof

A technology of cell adhesion and hydrogel, which is applied in the field of biomedical material preparation, can solve problems such as limiting the application in the medical field, and achieve the effects of preventing bacterial invasion, improving mechanical properties, and good biocompatibility

Inactive Publication Date: 2018-09-14
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medical hydrogels currently prepared still have some defects in their functional diversity, which limits their application in the medical field.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate into a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 4.5 , made into a solution with a mass concentration of 1%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above-mentioned sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass ratio of dopamine, continued to stir and react at 25°C for 12 hours, and then put it into a dialysis bag with a molecular weight cut-off of 3500Da. After dialysis with distilled water for 3 days, the retentate was freeze-dried for 72 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.

[0024] (2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain mass of poly...

Embodiment 2

[0027] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate in a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 5.5 , made into a solution with a mass concentration of 1.5%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass proportion of 3-aminophenylboronic acid, continued to stir and react at 25°C for 18 hours, and then loaded into a dialysis device with a molecular weight cut-off of 8000 After dialysis with distilled water for 5 days, the retentate was freeze-dried for 60 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.

[0028](2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain m...

Embodiment 3

[0031] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate in a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 6.5 , made into a solution with a mass concentration of 2%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass proportion of 3-aminophenylboronic acid, continued to stir and react at 25°C for 18 hours, and then loaded into a dialysis device with a molecular weight cut-off of 8000Da After dialysis with distilled water for 5 days, the retentate was freeze-dried for 48 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.

[0032] (2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain ...

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Abstract

The invention discloses a medical hydrogel with pH sensitivity, self-healing nature and cell adhesion and a preparation method thereof. 3-aminophenylboronic acid modified sodium alginate and I type collagen grafted carboxylic polyvinyl alcohol are cross-linked according to a certain proportion so as to prepare the hydrogel, wherein the modified sodium alginate has phenylboronic acid groups, the phenylboronic acid groups and diol of the polyvinyl alcohol form supermolecule dynamic bonds, the supermolecule bonds with pH sensitivity break at acidic pH and regrow at alkaline pH, so that the hydrogel has pH response and self-healing performance; the phenylboronic acid groups can combine with carbohydrates on biological cell membranes, such as glycolipids or glycoproteins, thereby enabling the hydrogel to attach to cells and improving the cell adhesion; the introduced polyvinyl alcohol improves the mechanical performance of the hydrogel, and the polyvinyl alcohol molecular chains form hydrogen bonds through a physical method of repeated freezing and thawing, thereby improving the mechanical strength of the hydrogel. The main raw materials of the hydrogel, sodium alginate and polyvinyl alcohol, have high biocompatibility, pH response and self-healing performance. The hydrogel can be widely used in fields of medicine slow release, wound repair and tissue engineering and the like.

Description

technical field [0001] The invention relates to a hydrogel with pH-sensitive self-healing property, cell adhesion and good biocompatibility and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] Hydrogel is a kind of functional polymer material with three-dimensional network structure, unique water absorption and water retention. Because of its good biocompatibility and bionic characteristics, it has attracted much attention and is widely used in wound dressings, medical drug carriers, biosensing and other fields. Traditional synthetic polymer hydrogels have good mechanical properties and have the functions of carrying and transporting, but they cannot control the drug concentration according to the needs of the receptors, and there are certain defects in cell adhesion and biocompatibility. , can not meet people's demand for medical development. Therefore, the development of new intelligent multifuncti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L5/04C08L29/04
CPCC08J3/075C08J2305/04C08J2429/04
Inventor 但卫华白忠祥但年华陈一宁
Owner SICHUAN UNIV
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