Medical hydrogel with pH sensitivity, self-healing nature and cell adhesion and preparation method thereof
A technology of cell adhesion and hydrogel, which is applied in the field of biomedical material preparation, can solve problems such as limiting the application in the medical field, and achieve the effects of preventing bacterial invasion, improving mechanical properties, and good biocompatibility
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Embodiment 1
[0023] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate into a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 4.5 , made into a solution with a mass concentration of 1%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above-mentioned sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass ratio of dopamine, continued to stir and react at 25°C for 12 hours, and then put it into a dialysis bag with a molecular weight cut-off of 3500Da. After dialysis with distilled water for 3 days, the retentate was freeze-dried for 72 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.
[0024] (2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain mass of poly...
Embodiment 2
[0027] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate in a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 5.5 , made into a solution with a mass concentration of 1.5%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass proportion of 3-aminophenylboronic acid, continued to stir and react at 25°C for 18 hours, and then loaded into a dialysis device with a molecular weight cut-off of 8000 After dialysis with distilled water for 5 days, the retentate was freeze-dried for 60 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.
[0028](2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain m...
Embodiment 3
[0031] (1) Preparation of 3-aminophenylboronic acid-modified sodium alginate: Weigh a certain amount of sodium alginate in a reaction kettle, add 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a pH of 6.5 , made into a solution with a mass concentration of 2%, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinyl with a mass ratio of 3 Imine (NHS) was dissolved in the above sodium alginate solution, activated at 25°C for 40 minutes, then added a certain mass proportion of 3-aminophenylboronic acid, continued to stir and react at 25°C for 18 hours, and then loaded into a dialysis device with a molecular weight cut-off of 8000Da After dialysis with distilled water for 5 days, the retentate was freeze-dried for 48 hours at a pressure of 0-20Pa and a temperature of -45°C to obtain 3-aminophenylboronic acid-modified sodium alginate.
[0032] (2) Preparation of polyvinyl alcohol grafted with collagen chains: Weigh a certain ...
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