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Synthesis method for near-infrared fluorescent probe copper nano-cluster and application of synthesis method

A technology of copper nanoclusters and fluorescent probes, which is applied in the field of detection of glycoproteins by copper nanoclusters, can solve the problems of complex detection process, expensive instruments, and long detection time, and achieve simple glycoproteins, uniform size, and simple process Effect

Inactive Publication Date: 2015-10-14
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have shortcomings such as long detection time, complicated detection process, and expensive instruments. The present invention utilizes the affinity between functionalized copper nanoclusters with unique optical properties and boronic acid groups in glycoproteins to detect the boronic acid groups in the system. Glycoprotein, which can realize rapid and sensitive detection of glycoprotein through the change of fluorescence spectrum of the reaction system

Method used

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  • Synthesis method for near-infrared fluorescent probe copper nano-cluster and application of synthesis method
  • Synthesis method for near-infrared fluorescent probe copper nano-cluster and application of synthesis method
  • Synthesis method for near-infrared fluorescent probe copper nano-cluster and application of synthesis method

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Experimental program
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Effect test

Embodiment 1

[0046] Synthesis of copper nanoclusters

[0047] Weigh 60 mg of bovine serum albumin and dissolve it in a beaker containing 10 mL of high-purity water. After dissolving, pipette 0.25 mL of CuCl 2 Solution (0.1 mol / L) was added to the reaction system. The solution was in the state of milky white hydrogel, and was stirred for 30 min at room temperature. Pipette 0.5 mL of hydrazine hydrate (pH=8), slowly inject into the reaction vessel, and continue to stir at room temperature for 5 h. The solution was pale yellow, which indicated the formation of copper nanoclusters. The synthesized copper nanoclusters were transferred to the treated dialysis bag for purification, high-purity water was changed every 4 hours, and the dialysis was performed for 24 hours. After the purification was completed, the copper nanoclusters were obtained by vacuum drying at 35 °C, and the fluorescence intensity was detected.

Embodiment 2

[0049] Synthesis of copper nanoclusters

[0050] Weigh 100 mg of bovine serum albumin and dissolve it in a beaker containing 10 mL of high-purity water. After dissolving, pipette 0.25 mL of CuCl 2 Solution (0.1 mol / L) was added to the reaction system. The solution was in the state of milky white hydrogel, and was stirred for 30 min at room temperature. Pipette 0.5 mL of hydrazine hydrate (pH=8), slowly inject into the reaction vessel, and continue to stir at room temperature for 5 h. The solution was pale yellow, which indicated the formation of copper nanoclusters. The synthesized copper nanoclusters were transferred to the treated dialysis bag for purification, high-purity water was changed every 4 hours, and the dialysis was performed for 24 hours. After the purification was completed, the copper nanoclusters were obtained by vacuum drying at 35 °C, and the fluorescence intensity was detected.

Embodiment 3

[0052] Synthesis of copper nanoclusters

[0053] Weigh 160 mg of bovine serum albumin and dissolve it in a beaker containing 10 mL of high-purity water. After dissolving, pipette 0.25 mL of CuCl 2 Solution (0.1 mol / L) was added to the reaction system. The solution was in the state of milky white hydrogel, and was stirred for 30 min at room temperature. Pipette 0.5 mL of hydrazine hydrate (pH=8), slowly inject into the reaction vessel, and continue to stir at room temperature for 5 h. The solution was pale yellow, which indicated the formation of copper nanoclusters. The synthesized copper nanoclusters were transferred to the treated dialysis bag for purification, high-purity water was changed every 4 hours, and the dialysis was performed for 24 hours. After the purification was completed, the copper nanoclusters were obtained by vacuum drying at 35 °C, and the fluorescence intensity was detected. The prepared copper nanoclusters have the highest fluorescence intensity.

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Abstract

The invention discloses a synthesis method for a near-infrared fluorescent probe copper nano-cluster and a detection method for glycoprotein. The near-infrared fluorescent probe copper nano-cluster is synthesized through a one-pot method, aminophenylboronic acid is used for functionally transforming the synthesized copper nano-cluster, and the functionalized copper nano-cluster is obtained. Due to the specific action of boric acid groups on aminophenylboronic acid and cis diol in glycoprotein, the fluorescence intensity of the copper nano-cluster is quenched. A fluorescent probe can detect glycoprotein at high specificity and sensitivity. Glycoprotein widely exists in mucus, plasma, cytoplasm and cell membranes, is tightly related to living organisms, is an important physiological active material, and is tightly related to certain genetic diseases, and therefore important practical significance is provided for detecting glycoprotein.

Description

[0001] This patent is supported by the General Project 21375095 of the National Natural Science Foundation of China, the National Excellent Doctoral Dissertation Author Special Fund FANEDD-201023, the Key Project of Tianjin Applied Basic Research Program 12JCZDJC21700, and the first-level project ZX110185 of Tianjin "131" Innovative Talent Training Project funding. technical field [0002] The invention belongs to the technical field of biological analysis and detection, and relates to a "green" synthesis method of near-infrared fluorescent probe copper nano clusters, its functionalization and the application of the functionalized copper nano clusters to glycoprotein detection. Background technique [0003] Metal nanoclusters are photoluminescent semiconductor nanoclusters, mainly including gold nanoclusters, silver nanoclusters, platinum nanoclusters and copper nanoclusters. Metal nanoclusters are fluorescent and water-soluble molecular-level aggregates composed of several ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00C09K11/58B82Y40/00G01N21/64G01N33/68
Inventor 李妍张菲李欣格
Owner TIANJIN NORMAL UNIVERSITY
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