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Colorimetric sensor for detecting lipopolysaccharide and preparation method and application thereof

A technology of colorimetric sensor and lipopolysaccharide, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in meeting the requirements of rapid analysis, long time-consuming rapid silver staining, poor precision and quantification, etc. Inexpensive, short response time, wide detection range effect

Inactive Publication Date: 2017-01-04
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme-linked immunoassay is relatively simple and economical, and can be used for semi-quantitative determination in an appropriate range of turbidity, but its defect is that the specificity is not strong
The rapid silver staining method has the characteristics of long time-consuming, cumbersome operation, poor precision and quantification
These methods are difficult to meet the requirements of daily rapid analysis

Method used

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  • Colorimetric sensor for detecting lipopolysaccharide and preparation method and application thereof
  • Colorimetric sensor for detecting lipopolysaccharide and preparation method and application thereof
  • Colorimetric sensor for detecting lipopolysaccharide and preparation method and application thereof

Examples

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Embodiment 1

[0039] In this example, see figure 1 , figure 2 with Figure 5 , a colorimetric sensor for detecting lipopolysaccharide, mainly composed of a kit, a solid-liquid separation device, an ultraviolet-visible spectrum analysis device, an analysis system and a control system, the control system controls the supply and detection data of the reagents in the kit input and output; modify 3-aminophenylboronic acid onto magnetic nanoparticles, and use a solid-liquid separation device to obtain MNPs magnetic nanoparticles covered with boronic acid recognition groups on the surface of magnetic nanoparticles, and prepare MNPs magnetic nanoparticles covered with boronic acid recognition groups Nanoparticle solution, as the first recognition reagent, is stored in the first kit; under acidic conditions, according to 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) Set mixing ratio of salt and hydrogen peroxide, mix 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt with...

Embodiment 2

[0063] This embodiment is basically the same as Embodiment 1, especially in that:

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Abstract

The invention discloses a colorimetric sensor for detecting lipopolysaccharide and a preparation method and application thereof. The sensor carries out detection through the ultraviolet-visible light spectrum technology. The sensor is characterized in that recognition of aminophenylboronic acid modified magnetic nano-particles to lipopolysaccharide, the catalytic oxidation activity, similar to that of peroxidase, of the magnetic nano-particles and the hindering effect of lipopolysaccharide lipid bilayerson on ion transport are utilized. High specific binding of 3-amino phenylboronic acid and lipopolysaccharide and the hindering effect of the lipopolysaccharide lipid bilayerson on ion transport are utilized, high specificity of detection is guaranteed, and detection sensitivity is improved through the catalytic oxidation activity, similar to that of peroxidase, of the magnetic nano-particles. Color changes generated in the magnetic nanocatalyzed oxidationof ABTS with H2O2 are captured through the ultraviolet-visible light spectrum technology, and analysis and detection of lipopolysaccharide are achieved. The method is simple, rapid and free of signal marking. Sensitivity is high and can linearly detect lipopolysaccharide within the range of 0.00001 microgram / mL to 180 microgram / mL, high specificity is achieved, and lipopolysaccharide and other contrast matter can be effectively distinguished.

Description

technical field [0001] The invention relates to a food hygiene quality detection process equipment, method and application, in particular to a bacterial endotoxin detection instrument, method and application, which are applied in the technical fields of food quality control and health management analysis. Background technique [0002] Lipopolysaccharide (LPS) is a component in the cell wall of Gram-negative bacteria, which is released when the bacteria die and dissolve or are artificially destroyed, so it is called endotoxin. Endotoxin consists of three parts: lipid A, core oligosaccharides and specific O-antigen polysaccharide side chains, and its toxic component is mainly lipid A. Lipid A is the glycolipid that constitutes endotoxin toxicity, covalently linked to the heteropolysaccharide chain. Endotoxin is located in the outermost layer of the cell wall and covers the mucous peptide of the cell wall, which can cause fever, microcirculation disturbance, endotoxin shock an...

Claims

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Application Information

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IPC IPC(8): G01N21/78B82Y30/00
CPCB82Y30/00G01N21/78G01N2021/751G01N2021/7756G01N2021/7769
Inventor 李根喜张娟李得凤
Owner SHANGHAI UNIV
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