Colorimetric sensor for detecting lipopolysaccharide and preparation method and application thereof
A technology of colorimetric sensor and lipopolysaccharide, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in meeting the requirements of rapid analysis, long time-consuming rapid silver staining, poor precision and quantification, etc. Inexpensive, short response time, wide detection range effect
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Embodiment 1
[0039] In this example, see figure 1 , figure 2 with Figure 5 , a colorimetric sensor for detecting lipopolysaccharide, mainly composed of a kit, a solid-liquid separation device, an ultraviolet-visible spectrum analysis device, an analysis system and a control system, the control system controls the supply and detection data of the reagents in the kit input and output; modify 3-aminophenylboronic acid onto magnetic nanoparticles, and use a solid-liquid separation device to obtain MNPs magnetic nanoparticles covered with boronic acid recognition groups on the surface of magnetic nanoparticles, and prepare MNPs magnetic nanoparticles covered with boronic acid recognition groups Nanoparticle solution, as the first recognition reagent, is stored in the first kit; under acidic conditions, according to 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) Set mixing ratio of salt and hydrogen peroxide, mix 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt with...
Embodiment 2
[0063] This embodiment is basically the same as Embodiment 1, especially in that:
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