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Nano mimic enzyme material, preparation method and application thereof, and method for detecting ovomucoid

A technology of ovomucoid and simulating enzymes, which is applied in the field of nano-simulating enzyme materials and its preparation, and detection of ovomucoids, which can solve the problems of fluorescent probes being easily interfered, affecting the detection sensitivity of ELSA, and difficult to quantify

Active Publication Date: 2022-04-08
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004]Traditional methods for detecting OM include fluorescence method and ELSA. Linked immunosorbent assay, this method can directly capture the ovomucoid with an antibody that has a specific reaction with ovomucoid, and carry out a chromogenic measurement through the antibody carrying the probe, but the fluorescent probe is easily interfered, and quantitative Difficult, affecting the detection sensitivity of ELSA method

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  • Nano mimic enzyme material, preparation method and application thereof, and method for detecting ovomucoid
  • Nano mimic enzyme material, preparation method and application thereof, and method for detecting ovomucoid
  • Nano mimic enzyme material, preparation method and application thereof, and method for detecting ovomucoid

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[0038] The present invention also provides a preparation method of the nano-mimetic enzyme material described in the above technical scheme, comprising the following steps:

[0039] Copper peroxide, 3-aminophenylboronic acid and water are mixed for coordination to obtain the nano-mimetic enzyme material.

[0040] In the present invention, unless otherwise specified, all preparation materials are commercially available products well known to those skilled in the art.

[0041] In the present invention, the copper peroxide is preferably obtained by preparation, and the preparation process of the copper peroxide preferably includes the following steps:

[0042]After mixing 0.05g of polyvinylpyrrolidone, 0.1g of copper chloride dihydrate and 5mL of deionized water, add 5mL of 0.02M sodium hydroxide solution and 0.1mL of 30% hydrogen peroxide solution, and stir at room temperature for 30 After ~40min to form a uniform brown solution, circulate water washing and centrifugation (the ...

Embodiment 1

[0084] After mixing 0.05g polyvinylpyrrolidone, 0.1g copper chloride dihydrate and 5mL deionized water, add 5mL sodium hydroxide solution with a concentration of 0.02M and 0.1mL hydrogen peroxide solution with a mass concentration of 30%, and stir at room temperature for 30min After forming a uniform brown solution, wash with circulating water and centrifuge (8000rpm, 8-10min) 3 times, and vacuum-dry the obtained precipitate at 60°C to obtain copper peroxide;

[0085] Disperse 10mg of copper peroxide and 10mg of 3-APBA in 10mL of water, stir overnight at room temperature to form a uniform dark brown solution, wash with circulating water and centrifuge three times, the centrifugal speed is 8000rpm, the time is 10min, the precipitate obtained Dry in a vacuum environment at 60°C to obtain the nano-mimetic enzyme material (referred to as CuO 2 @3-APBA);

[0086] Cover the ovomucoid antibody solution with a concentration of 1 μg / mL on the microtiter plate, incubate overnight at 4°...

Embodiment 2

[0097] After mixing 0.05g polyvinylpyrrolidone, 0.1g copper chloride dihydrate and 5mL deionized water, add 5mL sodium hydroxide solution with a concentration of 0.02M and 0.1mL hydrogen peroxide solution with a mass concentration of 30%, and stir at room temperature for 30min After forming a uniform brown solution, wash with circulating water and centrifuge (8000rpm, 8-10min) 3 times, and vacuum-dry the obtained precipitate at 60°C to obtain copper peroxide;

[0098] Disperse 10mg of copper peroxide and 10mg of 3-APBA in 10mL of water, stir overnight at room temperature to form a uniform dark brown solution, wash with circulating water and centrifuge three times, the centrifugal speed is 8000rpm, the time is 10min, the precipitate obtained Dry in a vacuum environment at 60°C to obtain the nano-mimetic enzyme material (referred to as CuO 2 @3-APBA);

[0099] Cover the ovomucoid antibody solution with a concentration of 1 μg / mL on the microtiter plate, incubate overnight at 4°...

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Abstract

The invention relates to the technical field of biosensing detection, in particular to a nano mimic enzyme material, a preparation method and application thereof and a method for detecting ovomucoid. The invention provides a nano mimic enzyme material. The nano mimic enzyme material comprises copper peroxide and 3-aminophenylboronic acid which is chemically combined with the copper peroxide. The copper peroxide in the nano mimic enzyme material disclosed by the invention has ultrahigh enzyme activity so as to improve the generation of photochemical signals; the 3-aminophenylboronic acid can be specifically combined with vicinal diol on an ovoid mucoprotein sugar chain; the 3-aminophenylboronic acid enables copper peroxide to have better dispersibility and increased catalytic active sites, not only plays a role in specific binding of target molecules (ovomucoid), but also amplifies ultraviolet visible light signals, and fully exerts the excellent performance of the 3-aminophenylboronic acid as a probe material.

Description

technical field [0001] The invention relates to the technical field of biosensing and detection, in particular to a nano-mimetic enzyme material, a preparation method and application thereof, and a method for detecting ovomucoid. Background technique [0002] Food allergy refers to a specific immune response that occurs in the body after the human body ingests a specific food. In recent years, the number of patients with food allergies is increasing, and the incidence of allergies is also increasing year by year. Among the allergic reactions, more than 90% are caused by eight types of foods, which are eggs, peanuts, milk, soybeans, wheat, tree nuts, shellfish and fish. Eggs are one of the most common foods that trigger allergic reactions. Proteins in eggs have been widely used in the raw food industry due to their unique processing properties, such as the foamability, emulsification, heating stability, and adhesiveness of egg white proteins, and ovomucoid is the main prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/22B01J31/02B82Y30/00B82Y40/00G01N21/33G01N21/78G01N33/543G01N33/68
Inventor 李灿鹏陈春兰赵卉
Owner YUNNAN UNIV
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