Dual-emission fluorescent probe for simultaneously detecting nitrite ions and Hg<2+> and method thereof

A technology of nitrite ion and double-emission fluorescence, which is applied in the direction of fluorescence/phosphorescence, preparation of test samples, measurement devices, etc., can solve the problem of insufficient sensitivity and achieve good consistency and selectivity

Active Publication Date: 2020-03-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ion chromatography and gas-phase molecular absorption spectrometry are simple, fast, and have less interference, and N-(1-naphthyl)ethylenediamine spectrophotometry has certain interference, but the sensitivity of these methods is not high enough

Method used

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  • Dual-emission fluorescent probe for simultaneously detecting nitrite ions and Hg&lt;2+&gt; and method thereof
  • Dual-emission fluorescent probe for simultaneously detecting nitrite ions and Hg&lt;2+&gt; and method thereof
  • Dual-emission fluorescent probe for simultaneously detecting nitrite ions and Hg&lt;2+&gt; and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this example, a dual-emission fluorescent probe based on carbon quantum dots is designed. The carbon quantum dots in the dual-emission fluorescent probe are mixed aqueous solutions of nicotinic acid and barbituric acid, which are placed in a high-pressure reactor and passed through It is prepared by hydrothermal reaction, and its specific synthesis method is as follows:

[0024] Weigh niacin (3.0mmol, 369.11mg) and barbituric acid (3.0mmol, 384.27mg) into a 50mL beaker, add 20mL deionized water, ultrasonically disperse for 15min, transfer the mixture to 100mL polytetrafluoroethylene In a lined autoclave, the reaction was carried out at 180 °C for 8 hours, and then allowed to cool naturally to room temperature. The reaction product was centrifuged at 10000rpm for 15min, the supernatant was taken, and further purified with a dialysis bag (1000Da) to obtain a light yellow quantum dot solution, which could be freeze-dried to obtain a carbon quantum dot solid powder produ...

Embodiment 2

[0028] In this example, based on the carbon quantum dots CQDs prepared in Example 1, a dual-emission fluorescent probe was constructed to detect NO 2 - and Hg 2 + Perform simultaneous detection:

[0029] A certain amount of glycine buffer solution (pH=7.5), CQDs solution, Tb(NO 3 ) 3 solution, APBA solution constitute CQDs-Tb 3+ / APBA dual emission fluorescent probe buffer solution system, and then add a certain concentration of NaNO to the buffer solution system 2 (5.0~5000.0nM) or Hg(NO 3 ) 2 (0.1 ~ 10.0μM) or a certain volume of pretreated water sample as the sample to be tested, and finally dilute to 10.0mL with ultrapure water. In the mixed solution after constant volume, the concentration of glycine is 20mM, the concentration of CQDs solution is 25~100mg / L, Tb(NO 3 ) 3 The concentration of the solution is 20.0 μM, the concentration of the APBA solution is 8-20 μM, shake well and stand at room temperature for 5 minutes. With 260-280nm as the excitation waveleng...

Embodiment 3

[0051] Based on the above-mentioned embodiments, this embodiment designs a method for simultaneously detecting nitrite ions and Hg using a dual-emission fluorescent probe. 2+ method, the method is:

[0052] 1) carbon quantum dots CQDs, Tb(NO) prepared in Example 1 3 ) 3 . APBA was added to the glycine buffer solution with pH = 7.5, and then the sample to be tested was quantitatively added to the buffer solution system, and finally the volume was adjusted to 10.0 mL with ultrapure water. At the same time, set up a set of buffer solution system without adding the sample to be tested, and also set the volume to 10.0mL as a blank sample. In the mixed solution after constant volume, the glycine concentration is 20mM, the CQDs solution concentration is 50mg / L, Tb(NO 3 ) 3 The concentration of the solution is 20.0 μM, the concentration of the APBA solution is 10 μM, shake well and stand at room temperature for 5 minutes.

[0053] 2) Taking 270nm as the excitation wavelength, sca...

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Abstract

The invention discloses a dual-emission fluorescent probe and a method for simultaneously detecting nitrite ions and Hg<2+>, which belong to the technical field of environmental detection. The fluorescent probe is a compound formed by carbon quantum dots CQDs, Tb<3+> and 3-aminophenylboronic acid in a buffer solution system with the pH value of 7.0 to 8.0. The carbon quantum dots CQDs are preparedfrom a mixed aqueous solution of nicotinic acid and barbituric acid through a hydrothermal reaction. The detection method comprises the steps of quantitatively adding a to-be-detected solution into the buffer solution system for constant volume, and then scanning an emission spectrum of a fluorescence system in a wavelength range of 320 to 650nm by taking 260 to 280nm as an excitation wavelengthof the buffer solution after constant volume; and calculating the concentrations of Hg<2+> and NO2<-> in the to-be-detected solution according to the linear relationship between the fluorescence quenching efficiency at 373 nm and 545 nm and the concentration of a to-be-detected substance. The fluorescent probe provided by the invention not only can realize trace detection of NO2<-> and Hg<2+> in awater sample, but also has better consistency and selectivity in detection results compared with a spectrophotometric method.

Description

technical field [0001] The invention relates to the relationship between nitrite ion and Hg in water sample 2+ The detection method of the method specifically relates to a kind of dual-emission fluorescent probe and the detection of nitrite ion and Hg 2+ The simultaneous detection method of the invention belongs to the technical field of environmental detection. Background technique [0002] Nitrite ion is an intermediate product of the nitrogen cycle. After nitrite enters the human body, it can oxidize low-iron hemoglobin to methemoglobin, making it lose the ability to transport oxygen, and can also react with secondary amines to generate carcinogenic sub- Nitamines. The commonly used determination methods of nitrite ion in water include ion chromatography, gas phase molecular absorption spectrometry and N-(1-naphthyl)ethylenediamine spectrophotometry. Ion chromatography and gas phase molecular absorption spectrometry are simple, fast, and have less interference. N-(1-na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/38
CPCG01N1/38G01N21/6402G01N21/643G01N2021/6432
Inventor 童裳伦武会芳
Owner ZHEJIANG UNIV
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