Gold nanoparticle-containing glucose visual detection sensor and its preparation method and use

A glucose sensor and gold nanoparticle technology, which is applied in the direction of analyzing materials through chemical reactions and analyzing materials through observing the impact on chemical indicators, can solve problems such as interference detection results and agglomeration

Active Publication Date: 2016-05-04
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since gold nanoparticles are nano-scale materials, they inevitably face the problem of agglomeration, especially when the salt solution with too high ion concentration exists in the detection environment, the agglomeration phenomenon is obvious, which seriously interferes with the detection results.

Method used

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  • Gold nanoparticle-containing glucose visual detection sensor and its preparation method and use
  • Gold nanoparticle-containing glucose visual detection sensor and its preparation method and use
  • Gold nanoparticle-containing glucose visual detection sensor and its preparation method and use

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preparation example Construction

[0019] The invention provides a method for preparing a gold nanoparticle visual detection glucose sensor, which is characterized in that, comprising:

[0020] 1) carry out contact reaction with chloroauric acid and trisodium citrate in solvent to obtain gold colloidal solution;

[0021] 2) Adjust the pH of the gold colloid solution to 6.0-7.5, then add 3-aminophenylboronic acid (APBA) and amino-terminated poly-N-isopropylacrylamide (PNIPAM-NH 2 ) for electrostatic interaction to prepare a gold nanoparticle visual detection glucose sensor.

[0022] In step 1) of the present invention, the specific type of solvent can be selected in a wide range, but considering cost, preferably, the solvent is deionized water.

[0023] In step 1) of the present invention, the consumption of each substance can be selected in a wide range, but in order to improve the productive rate of gold nanoparticles, preferably, with respect to the water of 100 weight parts, the consumption of chloroauric a...

Embodiment 1

[0032] 1) Prepare the gold colloid solution according to the method described in "FrensG.Controllednucleationfortheregulationoftheparticlesizeinmonodispersegoldsuspensions[J].Nature, 1973,241(105):20-22.": first use aqua regia (HCl: HNO 3 =3:1, volume ratio) to clean and remove impurities, rinse with deionized water, and dry naturally for use; then add 5mLHAuCl 4 (0.01mol / L) was added to 100mL of deionized water, magnetically stirred evenly; then the resulting solution was heated to reflux (100°C), and then 5mL of trisodium citrate solution (1% by weight) was quickly added to the above solution. It was observed that the color of the solution changed from colorless to purple and finally to wine red within a few minutes. Continue heating for 30 minutes to obtain a uniform citric acid stable gold colloid solution. Remove the heating device, cool at 25°C, and store in a sealed container. Store in a refrigerator at 4°C.

[0033] 2) At 25°C, take 0.5mL of gold colloid solution and ...

Embodiment 2

[0035] The sensor A2 was prepared according to the method of Example 1, except that the pH was adjusted to 6.0 in step 2).

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Abstract

The invention discloses a gold nanoparticle-containing glucose visual detection sensor and its preparation method and use. The preparation method comprises that 1, chloroauric acid and trisodium citrate undergo a contact reaction in a solvent to produce a colloidal gold solution, and 2, pH of the colloidal gold solution is adjusted to 6.0-7.5 and 3-aminophenylboronic acid (APBA) and amino-terminated poly(N-isopropylacrylamide) (PNIPAM-NH2) are added into the colloidal gold solution for electrostatic interaction so that the gold nanoparticle-containing glucose visual detection sensor is obtained. The sensor has good sensitivity, selectivity and ion interference resistance in the glucose detection. The preparation method can be operated simply and easily and the detection result can be read through naked eyes.

Description

technical field [0001] The invention relates to a visual sensor, in particular to a gold nanoparticle visual detection glucose sensor and its preparation method and application. Background technique [0002] The gold colloidal solution is the gold nanoparticle solution (AuNPs). Gold nanoparticles have a very high extinction coefficient, among which, the extinction coefficient of 13nm gold nanoparticles is as high as 2.7×10 8 mol / (L cm), which is more than 1000 times higher than that of ordinary dye molecules. According to the Beer-Lambert law, the detection limit that gold nanoparticles can achieve is far lower than that of dye molecules. On the other hand, since the gold nanoparticle system has different color changes in different states, it plays an important role in visual detection. The visual detection mechanism based on gold nanoparticles is: monodisperse gold nanoparticles appear red in the solution, when the detected substance is added, the gold nanoparticles aggre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 钟敏阚显文
Owner ANHUI NORMAL UNIV
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