Boric acid quantum dot ratio fluorescent probe and preparation method and application thereof

A ratiometric fluorescent probe and quantum dot technology, applied in chemical instruments and methods, fluorescence/phosphorescence, measuring devices, etc., can solve the problems of sample pretreatment and low sensitivity, large solvent consumption, time-consuming, etc., and achieve visual and rapid detection Blood glucose content, effect of good optical properties

Inactive Publication Date: 2016-04-06
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a boronated quantum dot ratio fluorescent probe for visually detecting glucose and its preparation method, which overcomes t

Method used

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  • Boric acid quantum dot ratio fluorescent probe and preparation method and application thereof
  • Boric acid quantum dot ratio fluorescent probe and preparation method and application thereof
  • Boric acid quantum dot ratio fluorescent probe and preparation method and application thereof

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Effect test

Embodiment 1

[0033] (1) 30.3mg sodium borohydride (NaBH 4 ) and 51.04mg of tellurium powder were added to the centrifuge tube, and then 3.0mL double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, the final white liquid was the desired The precursor NaHTe solution.

[0034] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 10.5 for nitrogen and deoxygenation 2 aqueous solution, to which 228.34 mg of CdCl was added 2 2.5H 2 O and 138.5 μL of TGA. The mixed solution was refluxed for 6 hours and 72 hours under nitrogen protection at 100° C. to obtain the required green fluorescent quantum dot solution and red fluorescent quantum dot solution.

[0035] (3) Add 400 μL of the red fluorescent quantum dot solution obtained in step (2) to a mixed solution of 15 mL of cyclohexane, 3 mL of n-hexanol and 3 mL of Trit...

Embodiment 2

[0038] (1) 60.6 mg sodium borohydride (NaBH 4 ) and 51.04mg of tellurium powder were added to the centrifuge tube, and then 3.0mL double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, the final white liquid was the desired The precursor NaHTe solution.

[0039] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 11.5 for nitrogen and deoxygenation 2 aqueous solution, to which 152.23 mg of CdCl was added 2 2.5H 2 O and 115.44 μL of TGA. The mixed solution was refluxed for 6 hours and 72 hours under nitrogen protection at 110° C. to obtain the desired green fluorescent quantum dot solution and red fluorescent quantum dot solution.

[0040] (3) Add 800 μL of the red fluorescent quantum dot solution obtained in step (2) to a mixed solution of 15 mL of cyclohexane, 4 mL of n-hexanol and 4 mL of Tri...

Embodiment 3

[0043] (1) Add 45.4mg sodium borohydride (NaBH 4 ) and 51.04mg of tellurium powder were added to the centrifuge tube, and then 3.0mL double distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the tube mouth was kept to vent gas, the final white liquid was the desired The precursor NaHTe solution.

[0044] (2) Inject the newly obtained precursor NaHTe into CdCl with thioglycolic acid (TGA) at a pH of 11.2 for nitrogen and deoxygenation 2 aqueous solution, to which 182.672 mg of CdCl was added 2 2.5H 2 O and 133 μL of TGA. The mixed solution was refluxed for 6 hours and 72 hours under nitrogen protection at 105° C. to obtain the required green fluorescent quantum dot solution and red fluorescent quantum dot solution.

[0045] (3) Add 600 μL of the red fluorescent quantum dot solution obtained in step (2) to a mixed solution of 15 mL of cyclohexane, 3.6 mL of n-hexanol and 3.6 mL of T...

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Abstract

The invention relates to a boric acid quantum dot ratio fluorescent probe and a preparation method and application thereof and belongs to the field of fluorescent probes and preparation thereof. The preparation method comprises the following steps: utilizing routine techniques to obtain required green fluorescent quantum dots and red fluorescent quantum dots; coating silicon balls with the red fluorescent quantum dots by the reverse microemulsion method, wherein under the action of poly dimethyl diallyl ammonium chloride, the green fluorescent quantum dots are adsorbed onto the surfaces of the silicon balls; finally, adding EDC/NHS and aminophenylboronic acid (APBA) into a Tris-HCl buffer solution; conducting reaction under the photophobic condition at the room temperature to finally obtain the boric acid quantum dot ratio fluorescent probe; detecting the content of glucose by fluorescent quantitation and visual analysis. The prepared boric acid quantum dot ratio fluorescent probe has favorable optical property and stability and has the visualized glucose detection capability.

Description

technical field [0001] The invention relates to a borated quantum dot ratio fluorescent probe and its preparation method and application, belonging to the field of fluorescent probes and its preparation. Background technique [0002] Diabetes is a common chronic metabolic disease. Together with cardiovascular disease and cancer, it is known as the three major killers of human health, which seriously threatens human health. According to the latest statistics from the International Diabetes Federation (IDF), about 366 million people worldwide suffer from diabetes, and 4.6 million people die of diabetes every year. It is estimated that by 2030, the number of people with diabetes worldwide will reach 550 million. my country is one of the countries with the fastest growing prevalence of diabetes, and the prevalence of diabetes is as high as 10%. Because diabetics lack the ability to regulate blood sugar, the glucose level in the body is easily outside the normal range. It is ea...

Claims

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Application Information

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IPC IPC(8): C09K11/88C09K11/02G01N21/64
CPCC09K11/025C09K11/883G01N21/6486
Inventor 郝桐帆卫潇聂仪晶周志平
Owner JIANGSU UNIV
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