Doped carbon quantum dot/PVA-based composite membrane, preparation method thereof and application in tetracycline detection
A technology of carbon quantum dots and composite membranes, applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of fluorescence quenching, inconvenient carrying of instruments, reduced detection sensitivity, etc., and achieve convenient operation and convenient vision. Visualize, prepare the effect with a simple method
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[0043] The third embodiment of the present invention provides a preparation method based on doped carbon quantum dots / PVA composite film. The hydrothermal method is used to make carbon quantum dots from 3-aminophenylboronic acid and citric acid, and polyethylene Alcohol is added to the aqueous solution of carbon quantum dots, a crosslinking agent is added to carry out crosslinking, and then the crosslinked material is removed from water to form a membrane material, that is, a composite membrane based on doped carbon quantum dots / PVA is obtained.
[0044] The present invention uses polyvinyl alcohol as a supporting carrier, not only because it has good mechanical and thermal properties and excellent barrier properties, but also because it has gel properties during the preparation process, which can better fix CDs in PVA, thereby It can better stabilize the fluorescence of CDs.
[0045] In some embodiments, after polyvinyl alcohol is added to the aqueous solution of carbon quant...
Embodiment 1
[0056] Synthetic method of tetracycline sensor
[0057] Briefly, 4.8 mg of 3-aminophenylboronic acid and 4.8 mg of citric acid were dissolved in 5 mL of deionized water, and after sonication for 2 min, the mixture was transferred to a 25 mL Teflon autoclave and placed in a vacuum drying oven. Heating at 180°C for 6h. After cooling to room temperature, the obtained solution was centrifugally purified in a centrifuge for 5 minutes to remove reaction residues and aggregated particles to obtain a supernatant. Finally, the obtained light yellow transparent solution was stored at 4°C for later use, and the CDs / PVA composite film was prepared by drop coating method. Add PVA to the CDs solution (the concentration of the CDs solution is 1.0mg / mL, after adding PVA, the concentration of PVA in the solution is 100mg / mL), then heat the PVA until it is completely dissolved, and then add it as a cross-linking agent to the solution of urea (65mg / mL). Stir with a glass rod for 10 s after ad...
Embodiment 2
[0085] Tetracycline sensor prepared in Example 1. Tetracycline (TC) at 250 μM, bovine serum albumin (BSA), ascorbic acid (AA), glucose (Glu), glutathione (GSH), cysteine (Cys), serine (Serine) at a concentration of 500 μM ), Lysine (Lys), Threonine (Thr), Histidine (His), Aspartic Acid (Asp), Mg 2+ 、K + 、Na + , Ca 2+ , Fe 3+ , Zn 2+ Test the fluorescence signal respectively. In addition, the influence of some potentially interfering antibiotics with similar structures was investigated. These substances include sulfadiazine (SD), doxycycline hydrochloride (DOX), oxytetracycline (OTC), streptomycin (STR), penicillin G, sodium (Pen G Na), erythromycin (ERY), hydrochloric acid Clindamycin (CLI), chloramphenicol (CHL), cefotaxime sodium (CTX), tetracycline hydrochloride (CTC) and azithromycin (AZM). TCs include TC, OTC, DOX and CTC (250μM), and the concentration of other substances is higher (500μM). Such as Figure 9 As shown, only TC can significantly quench the fluor...
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