Doped carbon quantum dot/PVA-based composite membrane, preparation method thereof and application in tetracycline detection

A technology of carbon quantum dots and composite membranes, applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of fluorescence quenching, inconvenient carrying of instruments, reduced detection sensitivity, etc., and achieve convenient operation and convenient vision. Visualize, prepare the effect with a simple method

Inactive Publication Date: 2021-06-29
LIAOCHENG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, the inventors have found that the current analysis of tetracycline detection by fluorescence methods is carried out in water media, and water media is not a sensor. Carbon quantum dots need to be configured into an aqueous solution. Preparation of carbon quantum dots into aqueous solution will cause fluorescence quenching due to aggregation of carbon quantum dots, resulting in reduced detection sensitivity

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  • Doped carbon quantum dot/PVA-based composite membrane, preparation method thereof and application in tetracycline detection
  • Doped carbon quantum dot/PVA-based composite membrane, preparation method thereof and application in tetracycline detection
  • Doped carbon quantum dot/PVA-based composite membrane, preparation method thereof and application in tetracycline detection

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Embodiment approach

[0043] The third embodiment of the present invention provides a preparation method based on doped carbon quantum dots / PVA composite film. The hydrothermal method is used to make carbon quantum dots from 3-aminophenylboronic acid and citric acid, and polyethylene Alcohol is added to the aqueous solution of carbon quantum dots, a crosslinking agent is added to carry out crosslinking, and then the crosslinked material is removed from water to form a membrane material, that is, a composite membrane based on doped carbon quantum dots / PVA is obtained.

[0044] The present invention uses polyvinyl alcohol as a supporting carrier, not only because it has good mechanical and thermal properties and excellent barrier properties, but also because it has gel properties during the preparation process, which can better fix CDs in PVA, thereby It can better stabilize the fluorescence of CDs.

[0045] In some embodiments, after polyvinyl alcohol is added to the aqueous solution of carbon quant...

Embodiment 1

[0056] Synthetic method of tetracycline sensor

[0057] Briefly, 4.8 mg of 3-aminophenylboronic acid and 4.8 mg of citric acid were dissolved in 5 mL of deionized water, and after sonication for 2 min, the mixture was transferred to a 25 mL Teflon autoclave and placed in a vacuum drying oven. Heating at 180°C for 6h. After cooling to room temperature, the obtained solution was centrifugally purified in a centrifuge for 5 minutes to remove reaction residues and aggregated particles to obtain a supernatant. Finally, the obtained light yellow transparent solution was stored at 4°C for later use, and the CDs / PVA composite film was prepared by drop coating method. Add PVA to the CDs solution (the concentration of the CDs solution is 1.0mg / mL, after adding PVA, the concentration of PVA in the solution is 100mg / mL), then heat the PVA until it is completely dissolved, and then add it as a cross-linking agent to the solution of urea (65mg / mL). Stir with a glass rod for 10 s after ad...

Embodiment 2

[0085] Tetracycline sensor prepared in Example 1. Tetracycline (TC) at 250 μM, bovine serum albumin (BSA), ascorbic acid (AA), glucose (Glu), glutathione (GSH), cysteine ​​(Cys), serine (Serine) at a concentration of 500 μM ), Lysine (Lys), Threonine (Thr), Histidine (His), Aspartic Acid (Asp), Mg 2+ 、K + 、Na + , Ca 2+ , Fe 3+ , Zn 2+ Test the fluorescence signal respectively. In addition, the influence of some potentially interfering antibiotics with similar structures was investigated. These substances include sulfadiazine (SD), doxycycline hydrochloride (DOX), oxytetracycline (OTC), streptomycin (STR), penicillin G, sodium (Pen G Na), erythromycin (ERY), hydrochloric acid Clindamycin (CLI), chloramphenicol (CHL), cefotaxime sodium (CTX), tetracycline hydrochloride (CTC) and azithromycin (AZM). TCs include TC, OTC, DOX and CTC (250μM), and the concentration of other substances is higher (500μM). Such as Figure 9 As shown, only TC can significantly quench the fluor...

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Abstract

The invention discloses a doped carbon quantum dot / PVA-based composite membrane, a preparation method thereof and application in tetracycline detection. The doped carbon quantum dot / PVA-based composite membrane comprises carbon quantum dots and a polyethylene hydrogel membrane, wherein the polyethylene hydrogel membrane loads the carbon quantum dots, and the carbon quantum dots are synthesized by using 3-aminophenylboronic acid and citric acid as precursors. The preparation method comprises the steps of preparing carbon quantum dots from 3-aminophenylboronic acid and citric acid by adopting a hydrothermal method, adding polyvinyl alcohol into an aqueous solution of the carbon quantum dots, adding a cross-linking agent for cross-linking, and removing water from a cross-linked material to prepare a membrane material, namely, the doped carbon quantum dot / PVA-based composite membrane. The composite membrane prepared by the invention can be used as a fluorescent sensor for detecting TC, is convenient to carry, has higher sensitivity and better selectivity for TC detection, and can be used for monitoring TC in real time.

Description

technical field [0001] The invention belongs to the field of sensor synthesis technology and photochemical analysis. The invention relates to a composite film based on doped carbon quantum dots / PVA, a preparation method thereof and an application for detecting tetracycline. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tetracyclines (TCs) are a group of antibiotics derived from the genus Streptomyces. Has broad-spectrum activity. The most commonly used tetracycline antibiotics in veterinary medicine are tetracycline, oxytetracycline, chlortetracycline and doxycycline. TC is a broad-spectrum antimicrobial drug. Due to its many side effects, the overuse of TC has attrac...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02B82Y20/00B82Y40/00G01N21/64C08L29/04C08K3/04C08J5/18
CPCB82Y20/00B82Y40/00C08J5/18C08J2329/04C08K3/04C09K11/025C09K11/65G01N21/6428G01N21/643G01N21/6456G01N2021/6432
Inventor 岳巧丽胡莹莹
Owner LIAOCHENG UNIV
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