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Ratiometric fluorescent probe, and preparation method and application thereof

A ratiometric fluorescent probe, fluorescent probe technology, applied in the field of drug detection, can solve the problems of high labor and time consumption, uncertainty of test results, relying on manual operation, etc., to achieve low cost, avoid the interference of human factors, intelligent The effect of increasing the degree of

Active Publication Date: 2019-12-20
ANHUI UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] In addition, the current drug detection technology has a low degree of intelligence, and data analysis and storage rely on manual operations
Especially in the face of a large number of anti-drug screening, drug rehabilitation monitoring, etc., it often consumes a lot of manpower and time, and there may be uncertainty in the test results due to human factors. This detection mode can no longer meet the current "Internet + intelligence" The needs of the era of "chemical testing"

Method used

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  • Ratiometric fluorescent probe, and preparation method and application thereof
  • Ratiometric fluorescent probe, and preparation method and application thereof
  • Ratiometric fluorescent probe, and preparation method and application thereof

Examples

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Embodiment 1

[0041] 1. Materials

[0042] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0043] 2. Method

[0044] 2.1 Preparation of fluorescent graphene oxide

[0045] Graphene oxide is placed in a mixed solution of N,N-dimethylformamide and thionyl chloride at a volume ratio of 1:1, and heated to reflux at a temperature of 60-100°C for 24-72h, and the obtained solid product is After washing with anhydrous tetrahydrofuran, centrifuging, and vacuum drying, add it to n-butylamine solution, and react at 50-70°C for 48-96h. After the product is separated by ultrapure water, it is the raw material of fluorescent graphene oxide, and it is stored at 4°C. stand-by.

[0046] 2.2 Preparation of copper nanocluster powder

[0047] Mix the copper salt and the carboxyl-containing complexing agent in ultrapure water,...

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Abstract

The invention relates to a ratiometric fluorescent probe, and a preparation method and application thereof. According to the invention, firstly, a fluorescent probe layer taking fluorescent graphene oxide and copper nanoclusters as raw materials is prepared, and a probe carrier is printed or coated with the fluorescent probe layer to obtain the ratiometric fluorescent probe; the concentration value of an aniline drug sample can be obtained by detecting an RGB value of the aniline drug on the surface of the ratiometric fluorescent probe and substituting the RGB value into a standard curve. According to the invention, drug detection is based on a specific combination reaction of special amino groups of the aniline drug and surface carboxyl groups of the copper nanoclusters, and detection sensitivity and accuracy are high.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a ratio fluorescent probe and its preparation method and application. Background technique [0002] In recent years, new types of drugs have emerged in an endless stream in my country, the number of drug users has soared sharply, the methods of drug crimes have diversified, and drug-related cases have increased year by year. The proliferation of drugs and drug crimes have become a major cancer that hinders the healthy development of my country's economy and destroys social stability and harmony. At present, the situation of drug crimes in our country is very severe. Therefore, accurate, rapid and comprehensive drug detection has very important practical significance for combating drug crimes, detecting drug cases, and curbing the spread of drugs. [0003] However, most of the commonly used drug detectors are based on chromatography and immunoassay methods. The...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/65G01N21/64
CPCC09K11/06C09K11/65G01N21/6428C09K2211/188
Inventor 杜袁鑫李琳
Owner ANHUI UNIVERSITY
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