Preparation method of graphene oxide fluorescence-enhanced functional nucleic acid hydrogel

Abstract

The invention discloses a preparation method of graphene oxide fluorescence-enhanced functional nucleic acid hydrogel. According to the method, the efficiency of preparing the DNA hydrogel is improvedthrough a hyperbranched rolling cycle amplification (HRCA) technology, and meanwhile, a functional nucleic acid sequence is designed in a template sequence of an HRCA reaction, so a template capableof forming a fluorescent metal nano-cluster such as a copper nano-cluster (CuNCs) is provided for the prepared hydrogel; and moreover, a graphene oxide (GO) nanomaterial is introduced into a preparation system, so the influence of an HRCA reaction system on CuNCs fluorescence is reduced, and the fluorescence intensity of CuNCs is remarkably enhanced. By adding GO and forming CuNCs, the compactnessand viscoelasticity of a hydrogel network are improved, and meanwhile, a new morphology is provided for a microstructure based on nanoflowers. According to the invention, the GO fluorescence-enhancedfunctional nucleic acid hydrogel with enhanced fluorescence and strong universality is rapidly prepared, and has very good application prospects in the aspects of molecular detection, biological imaging and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a preparation method of graphene oxide fluorescence-enhanced functional nucleic acid hydrogel. Background technique [0002] The preparation of DNA hydrogel by traditional linear rolling circle amplification (RCA) technology has problems such as long time-consuming, low mechanical strength and poor availability of directly obtained RCA hydrogel. At the same time, the enzymes, dithiothreitol, ATP and other substances in the RCA reaction system have a great inhibitory effect on the fluorescence intensity of the fluorescent functional nucleic acid hydrogel, which greatly limits the development and expansion of applications. In addition, the microscopic morphology of nucleic acid hydrogels obtained based on linear RCA technology is single, and it is difficult to control its size and morphology during the preparation process. Therefore, replacing linear RCA technology ...

AI Technical Summary

Problems solved by technology

[0002] The preparation of DNA hydrogel by traditional linear rolling circle amplification (RCA) technology takes a long time, and the directly obtained RCA hydrogel has low mechanical strength and poor availability.

At the same time, the enzymes, dithiothreitol, ATP and other substances in the RCA reaction system have a great inhibitory effect on the fluorescence intensity of the fluorescent functional nucleic acid hydrogel, which greatly limits the development and expansion of applications.

In addition, the microscopic morphology of nucleic acid hydrogels obtained based on linear RCA technology is single, and it is difficult to control its size and morphology during the preparation process.

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