Double-fluorescence-emission copper nano-cluster/carbon dot colorimetric probe, preparation method and application in trace water detection

A technology of copper nano-clusters and colorimetric probes, which is applied in the field of colorimetric sensing, can solve problems affecting detection results, etc., and achieve the effects of obvious detection effect, short time consumption, and simple preparation method

Inactive Publication Date: 2018-09-04
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the invented fluorescent probes are based on single-emission detection, which means that many factor

Method used

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  • Double-fluorescence-emission copper nano-cluster/carbon dot colorimetric probe, preparation method and application in trace water detection
  • Double-fluorescence-emission copper nano-cluster/carbon dot colorimetric probe, preparation method and application in trace water detection
  • Double-fluorescence-emission copper nano-cluster/carbon dot colorimetric probe, preparation method and application in trace water detection

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Synthesis of blue fluorescent CDs: Dissolve 1.0507g of citric acid and 335μL of ethylenediamine in 10mL of deionized water. Then the mixed solution was added to the Teflon reaction kettle, heated to 200° C. and reacted for 5 hours. After the reaction, it was naturally cooled to room temperature. The product is a brown-black transparent solution, a yellow solution is obtained after dialysis, and a light yellow CDs powder is obtained after freeze-drying.

[0031] (2) Synthesis of glutathione-modified CDs: 0.1995g glutathione (GSH) was added to 10mL of PBS (pH=6.86) buffer solution, and 1-(3-dimethylaminopropyl) was added to it )-3-Ethylcarbodiimide (EDC) (0.125g) and N-hydroxysuccinimide (NHS) (0.075g), the mixed solution was stirred at room temperature for 30min, protected from light, added 10mL to the concentration range of The 10 mg / mL CDs solution obtained in step (1) was then stirred at room temperature and protected from light for 10 hours, so that GSH-modified CD...

Embodiment 2

[0034] (1) Synthesis of blue fluorescent CDs: Dissolve 0.9603 g of citric acid and 300 μL of ethylenediamine in 8 mL of deionized water. Then the mixed solution was added to the Teflon reaction kettle, heated to 180°C and reacted for 6.5 hours, after the reaction, it was naturally cooled to room temperature. The product is a brown-black transparent solution, a yellow solution is obtained after dialysis, and a light yellow CDs powder is obtained after freeze-drying.

[0035] (2) Synthesis of glutathione-modified CDs: 0.1536g glutathione (GSH) was added to 8mL of PBS (pH=6.86) buffer solution, and 1-(3-dimethylaminopropyl) was added to it )-3-Ethylcarbodiimide (EDC) (0.0904g) and N-hydroxysuccinimide (NHS) (0.054g), the mixed solution was stirred at room temperature for 40min, protected from light, added 8mL to the concentration range of The 9 mg / mL CDs solution obtained in step (1) was then stirred at room temperature for 12 hours in the dark, so that GSH-modified CDs (GSH-CDs) w...

Embodiment 3

[0039] (1) Synthesis of blue fluorescent CDs: Dissolve 1.2359g of citric acid and 400μL of ethylenediamine in 12mL of deionized water. Then the mixed solution was added to the Teflon reaction kettle, heated to 160° C. for 8 hours of reaction, and after the reaction was over, it was naturally cooled to room temperature. The product is a brown-black transparent solution, a yellow solution is obtained after dialysis, and a light yellow CDs powder is obtained after freeze-drying.

[0040] (2) Synthesis of glutathione-modified CDs: 0.2033g glutathione (GSH) was added to 12mL of PBS (pH=6.86) buffer solution, and 1-(3-dimethylaminopropyl) was added to it )-3-Ethylcarbodiimide (EDC) (0.1652g) and N-hydroxysuccinimide (NHS) (0.947g), the mixed solution was stirred at room temperature for 60min, protected from light, added 12mL to the concentration range of The 12 mg / mL CDs solution obtained in step (1) was then stirred at room temperature and protected from light for 15 hours, so that G...

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Abstract

The invention relates to an in-situ preparation method of a copper nano-cluster/carbon dot (Cu NCs/CDs) double-fluorescence-emission colorimetric probe based on AIEE, and application thereof in detecting trace water in an organic solvent, which belong to the technical field of colorimetric sensing. The method comprises three steps of synthesizing blue-fluorescence carbon dots (CDs), carrying out surface modification on the CDs, and preparing the double-fluorescence-emission Cu NCs/CDs probe. The double-fluorescence-emission probe Cu NCs/CDs obtained by the method shows a double-fluorescence-emission effect with 450nm blue light and 596nm red light in a visible region under the 365nm ultraviolet excitation. By utilizing different responses of double fluorescence on different water contentsin the organic solvent, different composite fluorescence color changes are displayed, so that double-fluorescence-emission test paper can be used for colorimetric detection on trace water in the organic solvent through naked eyes, the detection limit can be lowered to 0.02 percent (v/v), and convenience is provided for detecting the trace water in environmental protection, food safety and organicreaction.

Description

Technical field [0001] The invention belongs to the technical field of colorimetric sensing, and specifically relates to the preparation of a dual emission colorimetric probe based on copper nanoclusters / carbon dots and its application in the detection of trace water in organic solvents. Background technique [0002] The water content in organic solvents will affect the progress of chemical reactions to a large extent, and even determine the reaction products, so sensitive and rapid detection of trace water in organic solvents is important for pharmaceutical manufacturing, environmental monitoring and food testing, especially organic synthesis It is of great significance. Therefore, the development of convenient and efficient water detection methods has aroused great interest. The commonly used chemical analysis method for the determination of water content in organic solvents is the classic Karl Fischer method, which was proposed in 1935. Although it has been greatly improved, ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 林权宋善良王传洗杨明辉赵月赵玥琪刘厚杨柏
Owner JILIN UNIV
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