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High-fluorescence copper nanocluster-cerium (III) fluorescent probe as well as preparation method and application thereof

A technology of fluorescent copper nanoclusters and copper nanoclusters is applied in the field of fluorescence sensing to achieve the effects of good selectivity, high selectivity and wide linear range.

Active Publication Date: 2020-08-11
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using cerium (III) ions as an inducer to promote the aggregation induction effect of copper nanoclusters to prepare highly fluorescent copper nanoclusters-cerium (III) fluorescent probes, and use them to construct fluorescent sensors for hydrogen peroxide and glucose Visual detection of hydrogen oxide and glucose has not been reported

Method used

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  • High-fluorescence copper nanocluster-cerium (III) fluorescent probe as well as preparation method and application thereof
  • High-fluorescence copper nanocluster-cerium (III) fluorescent probe as well as preparation method and application thereof
  • High-fluorescence copper nanocluster-cerium (III) fluorescent probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of copper nanoclusters: 10 mL of glutathione (50 mg / mL) was added dropwise to 10 mL of CuSO 4 (10 mM) in a round-bottomed flask (50 mL) at 37° C. for 1 hour at 650 rpm to generate a milky white suspension. Then adjust the pH of the above-mentioned reaction solution with NaOH (1M) solution, adjust the pH to 5 with reference to pH test paper, the color of the solution turns light yellow, continue to react for 2 hours, and the prepared copper nanoclusters are stored in a refrigerator at 4°C. Depend on figure 1 It can be seen that the prepared copper nanoclusters have a particle size of about 2 nm and are uniformly dispersed.

[0030] Preparation of copper nanoclusters-cerium (III) fluorescent probe: Add 600 μL of acetic acid-sodium acetate buffer solution (pH 5.6) and 100 μL of the copper nanoclusters prepared in Example 1 to a 1 mL centrifuge tube, vortex to mix, and Add 100 μL of cerium (III) ions (20 mM) to the mixture and react for 10 min.

Embodiment 2

[0032] Copper nanoclusters prepared by the method for preparing copper nanoclusters in Example 1.

[0033] Preparation of copper nanoclusters-cerium (III) fluorescent probe: Add 600 μL of acetic acid-sodium acetate buffer solution (pH 3.6) and 100 μL of the copper nanoclusters prepared in Example 1 to a 1 mL centrifuge tube, vortex to mix, and Add 100 μL of cerium (III) ions (10 mM) to the mixture and react for 10 min.

Embodiment 3

[0035] Copper nanoclusters prepared by the method for preparing copper nanoclusters in Example 1.

[0036] Preparation of copper nanoclusters-cerium (III) fluorescent probe: Add 600 μL of acetic acid-sodium acetate buffer solution (pH 3.6) and 100 μL of the copper nanoclusters prepared in Example 1 to a 1 mL centrifuge tube, vortex to mix, and Add 100 μL of cerium (III) ions (20 mM) to the mixture and react for 10 min. Such as figure 2 As shown, when 20 mM cerium (III) was added to the copper nanocluster solution to form copper nanocluster-cerium (III), its fluorescence was enhanced by 40 times compared with copper nanocluster.

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Abstract

The invention discloses a high-fluorescence copper nanocluster-cerium (III) fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of fluorescence sensing. Based on the phenomenon that hydrogen peroxide can enable fluorescence of the copper nano-cluster-cerium (III) fluorescent probe to be subjected to linear quenching, the copper nano-cluster-cerium (III) fluorescent probe is used for constructing a hydrogen peroxide and glucose fluorescent sensor. The method for enhancing the fluorescence characteristic of the glutathione-stabilized copper nanocluster by adopting the metal cation cerium (III) is simple to operate and remarkable in fluorescence enhancement degree, has the characteristics of high response speed and wide linear range when being used for detecting hydrogen peroxide and glucose, and can realize visual detection of hydrogen peroxide and glucose.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensing, and specifically refers to a high-fluorescence copper nanocluster-cerium (III) fluorescent probe, a preparation method thereof, and an application in the detection of hydrogen peroxide and glucose. Background technique [0002] Diabetes is clinically characterized by persistent high blood sugar, which can damage or fail the functions of various tissues and organs (eyes, kidneys, heart, etc.). Due to the lack of effective treatment methods, only real-time monitoring and strict control of blood sugar concentration can reduce the occurrence of complications. Hydrogen peroxide is a representative type of oxygen active species. Excessive hydrogen peroxide in the body can cause various biological damages, such as aging, Alzheimer's disease, neurodegeneration, and even cancer. Therefore, the development of highly sensitive, highly selective, and rapid glucose and hydrogen peroxide detectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6417
Inventor 梅鹤王学东周佩佩黄宏廖忠鹭聂天银吕梦雨马伟
Owner WENZHOU MEDICAL UNIV
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