SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as preparation method and detection method thereof

A detection method and sensor technology, which can be used in instruments, measuring devices, scientific instruments, etc., can solve problems such as reports of unseen ion detection, and achieve the effects of excellent uniformity and stability, short time consumption, and simple preparation.

Inactive Publication Date: 2016-06-22
NANJING UNIV OF POSTS & TELECOMM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In the Raman detection using the same substrate,...

Method used

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  • SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as preparation method and detection method thereof
  • SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as preparation method and detection method thereof
  • SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as preparation method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 110n

[0047] Embodiment 110nM oligonucleotide probe chain modification assembled SERS mercury ion detection substrate

[0048] (1) The silver nanorod array SERS substrate was rinsed several times with ultrapure water;

[0049] (2) Take 20uLCy5 modified oligonucleotide probe chain (10nM) and drop it on the SERS substrate, let it stand for 3 hours at 25°C and 80% humidity environment, and then rinse it with pure water; the SERS substrate of the modified probe chain Soak in buffer solution (1mM phosphate, 10mM sodium chloride, pH7.4) for 10min to keep the DNA form stable;

[0050] (3) Working curve: Dilute the 0.01M mercury standard solution with pure water to different concentrations of 1pM, 10pM, 100pM, 1nM, 10nM, 100nM, 1?M mercury ion sample solution; test the initial signal intensity of the SERS sensor, and the mercury The ion SERS sensor is placed in different concentrations of mercury ion solution to be detected, and reacted at room temperature for 3 hours; after taking out the...

Embodiment 250n

[0054] Example 250nM oligonucleotide probe chain modification assembled SERS mercury ion detection substrate

[0055] (1) The silver nanorod array SERS substrate was rinsed several times with ultrapure water;

[0056] (2) Take 20uLCy5 modified oligonucleotide probe chain (50nM) and drop it on the SERS substrate, let it stand for 3 hours at 25°C and 80% humidity environment, and then rinse it with pure water; the SERS substrate of the modified probe chain Soak in buffer solution (1mM phosphate, 10mM sodium chloride, pH7.4) for 10min to keep the DNA form stable;

[0057] (3) Working curve: Dilute the 0.01M mercury standard solution with pure water to different concentrations of 1pM, 10pM, 100pM, 1nM, 10nM, 100nM, 1?M mercury ion sample solution; test the initial signal intensity of the SERS sensor, and the mercury The ion SERS sensor is placed in different concentrations of mercury ion solutions to be detected, and reacted at room temperature for 3 hours; after taking out the s...

Embodiment 3100n

[0062] Embodiment 3100nM oligonucleotide probe chain modification assembled SERS mercury ion detection substrate

[0063] (1) The silver nanorod array SERS substrate was rinsed several times with ultrapure water;

[0064] (2) Take 20uLCy5 modified oligonucleotide probe chain (100nM) and drop it on the SERS substrate, let it stand for 3 hours at 25°C and 80% humidity environment, and then rinse it with pure water; the SERS substrate of the modified probe chain Soak in buffer solution (1mM phosphate, 10mM sodium chloride, pH7.4) for 10min to keep the DNA form stable;

[0065] (3) Working curve: Dilute the 0.01M mercury standard solution with pure water to different concentrations of 1pM, 10pM, 100pM, 1nM, 10nM, 100nM, 1?M mercury ion sample solution; test the initial signal intensity of the SERS sensor, and the mercury The ion SERS sensor is placed in different concentrations of mercury ion solution to be detected, and reacted at room temperature for 3 hours; after taking out t...

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Abstract

The invention discloses an SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as a preparation method and a detection method thereof. The sensor is obtained by modifying an oligonucleotide probe chain to the surface of a solid SERS substrate, wherein the oligonucleotide probe chain is composed of three parts; and one end of the oligonucleotide probe chain is modified by sulfydryl and is connected with a base, the other end of the oligonucleotide probe chain is modified by Raman dyestuff, and the middle part is composed of 5-25 pure thymine. The oligonucleotide probe chain is 5'-SH-(CH2)6-TTT TTT TTT TTT TTT-Cy5-3'. The solid SERS substrate is a silver nano rod array type SERS substrate. When the SERS sensor is used, the sensor is directly immersed into a solution to be detected; and when the sample contains the mercury ions, the mercury ions can be specifically combined with an oligonucleotide probe, so that the aim that the mercury ions are detected through detecting the intensity change of an SERS signal is realized. The mercury ion SERS sensor is simple and convenient in preparation process, short in preparation time and easy to carry, can realize rapid detection of the mercury ions and has good selectivity; and the sensor has no obvious interference on other metal ions and has high sensitivity, and the detection limit can reach the magnitude order of -0.1pM.

Description

technical field [0001] The invention belongs to the technical field of Raman spectrum analysis, and in particular relates to a SERS sensor for mercury ion detection and a preparation and detection method thereof. Background technique [0002] Mercury is a heavy metal ion with strong toxicity, which exists widely in the world. Its pollution will seriously endanger the environment and pose a great threat to human health. Accumulation of mercury in the human body can lead to permanent brain damage and other serious clinical symptoms. In addition, high levels of mercury in the mother's body can cause birth defects in babies. Therefore, the monitoring of mercury pollution is very necessary. [0003] Water-soluble divalent mercury ions (Hg 2+ ) is one of its most common, stable and widely transmitted forms, and one of the main sources of human exposure to mercury pollution. Due to the increase of human industrial mining activities and the surge of man-made mercury pollution emi...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 宋春元汪联辉杨博玥杨琰君
Owner NANJING UNIV OF POSTS & TELECOMM
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