The application provides a
nucleic acid detection card box and a
nucleic acid detection method, which comprise sample cavities, pipetting cavities and
reagent cavities arranged at intervals along the length direction of the box body; a
reaction tube provided with a reaction cavity; a control valve accommodated in a valve cavity and provided with a plurality of independent pipetting flow channels, the pipetting flow channels being used for connecting one of the sample cavities, the
reagent cavities and the reaction cavity with the pipetting cavity; and a pipetting driving element arranged in the pipetting cavity and capable of ascending and descending in the pipetting cavity to form
positive pressure or negative pressure in the pipetting cavity. The application integrates a plurality of pipetting flow channels in the control valve, simplifies the
nucleic acid detection structure, does not need to arrange other flow channel structures, and can realize the change of the flow channel by driving the control valve to rotate, realizes the
miniaturization of the
nucleic acid detection card box, realizes
liquid transfer and mixing by cooperating with the ascending and descending of the pipetting driving element, and can ensure that the
nucleic acid detection card box always maintains a fully-closed detection state during the pipetting and mixing process.