The invention discloses a preparation method of an epoxy resin glass fiber flute and belongs to the technical field of flute processing. The preparation method of the epoxy resin glass fiber flute comprises the following steps of: (1) weighing raw materials, and (2) conducting composite winding on the raw materials. The flute manufactured by the preparation method of the epoxy resin glass fiber flute is far lower than bamboo in temperature influence, shrinkage expansion coefficient and the like, the influence of the temperature on the flute (international standard pitch) is reduced to the minimum, the bamboo is replaced, the tone is excellent, the problems that the folk music wind instrument flute is cracked and the pitch of the original flute is influenced by the temperature are solved, the flute is hard, durable, not bent, poisonless and tasteless, the appearance and texture of the flute are beautiful, the bamboo wood is saved, and the national forest resources are protected. According to the preparation method of the epoxy resin glass fiber flute, the proportion is adjusted again, the problems of fluffing and wire drawing are solved, notches are flat and smooth, materials are not affected at all, the tone of the made flute is crisp, fine, smooth and soft and is very close to that of a flute made of bamboo, and the flute body also vibrates very well.