Concentration and purification device for antibacterial peptide

Abstract

The invention discloses an antibacterial peptide concentration and purification device, and particularly relates to the technical field of concentration and purification device.The antibacterial peptide concentration and purification device comprises a sewage tank and a liquid storage tank, a filtering part is fixed to the upper side of the sewage tank, a water storage tank is fixed to one side of the liquid storage tank, a first water pump is fixed to the upper side of the liquid storage tank, and a concentration part is fixed to the upper side of the water storage tank; a purification assembly is mounted on one side of the concentration part. According to the concentration and purification device for the antibacterial peptide, broken cell sap can be tangentially filtered through the arranged filtering parts, macromolecular substances such as cell debris and lipid are prevented from being deposited on the surface of a membrane, and the multiple filtering assemblies can be backwashed, so that the cell debris attached to the surface of the membrane in the filtering assemblies is reduced, and the concentration and purification efficiency of the antibacterial peptide is improved. The problem of frequent replacement caused by blockage of the filtering membrane is avoided, meanwhile, impurities in the filtered supernatant can be less, and the influence on the product quality is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of concentration and purification devices, in particular to a concentration and purification device for antimicrobial peptides. Background Art

[0002] Antimicrobial peptides are small molecule amino acid polymers produced through biotechnology that have shown significant effects in treating viral diseases. Some antimicrobial peptide technologies have entered clinical trials, and in the near future, antimicrobial peptide drugs are expected to be widely used in the treatment of malignant diseases.

[0003] During the concentration and purification process of antimicrobial peptides, the filter membrane is easily clogged by cell debris, and frequent replacement will increase the cost of operation. If it is not cleaned, the filtered antimicrobial peptide filtrate will contain too many impurities, affecting the quality of the product. In addition, during the concentration process, the flux decreases due to the adsorption of impurities on the surface of the ultrafiltration membrane, requiring frequent cleaning. The solute concentration gradient on the membrane surface further aggravates membrane contamination and shortens the membrane life. Summary of the Invention

[0004] The present invention aims to solve the problems in the concentration and purification of antimicrobial peptides, such as the clogging of deep filtration membranes by cell debris, which frequently increases operating costs, and excessive impurities in the filtered antimicrobial peptide filtrate if not cleaned, which affects product quality. In addition, during the concentration process, the ultrafiltration membrane surface adsorbs impurities, resulting in a decrease in flux and the need for frequent cleaning.

[0005] In order to achieve the above objectives, the present invention adopts the following technology: a device for concentrating and purifying antimicrobial peptides:

[0006] It includes a sewage tank and a liquid storage tank, a filter component is fixed on the upper side of the sewage tank, a water storage tank is fixed on one side of the liquid storage tank, a water pump is fixed on the upper side of the liquid storage tank, a concentration component is fixed on the upper side of the water storage tank, and a purification component is installed on one side of the concentration component;

[0007] The sewage tank includes a filter box and a water pump 2 fixedly connected to the filter box. The lower side of the filter box is connected to the inner cavity of the liquid storage tank through multiple delivery pipes. The inner cavity of the filter box is fixed with multiple evenly distributed fixed blocks, and the multiple fixed blocks are inlaid with filter components. Two adjacent filter components are connected by a connecting pipe, and the two filter components on both sides are fixedly connected to the three-way pipe 1 and the two-way pipe 2 respectively.

[0008] A tangential filtering flow loop is formed between the multiple filter components through the three-way pipe 1 and the connecting pipe, and the clean water in the water storage tank is extracted by the water pump 2 to backwash the multiple filter components.

[0009] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0010] The vertical portion of the first tee is connected to an electrically controlled valve, one side of which is in communication with the inner cavity of the sewage tank through a delivery pipe. The horizontal portion of the second tee is fixed with an electrically controlled valve on a side away from the filter assembly, one side of which is in communication with the inner cavity of the sewage tank through a delivery pipe. The vertical portion of the second tee is connected to the output end of the second water pump through a delivery pipe, and the input end of the second water pump is in communication with the inner cavity of the water tank through a delivery pipe.

[0011] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0012] A driving motor is fixed to one side of the filter box, a connecting shaft is fixed to the output end of the driving motor, a plurality of winding rollers adapted to the filter assembly are fixed to the outer surface of the connecting shaft, and two sets of connecting ropes are fixed to the outer surfaces of the plurality of winding rollers.

[0013] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0014] The filter assembly includes a fixed ring, and the inner surface of the fixed ring is provided with two symmetrical filter membranes and two symmetrical filter screens. The two filter screens are located on the inner sides of the two filter membranes, and the two filter screens are fixedly connected to the connecting shaft. Two groups of symmetrical brush strips are fixed on the inner surface of the fixed ring, and the brush strips in the same group are tightly attached to the surface of the filter screen on the same side.

[0015] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0016] Two symmetrical mounting plates are fixed to the inner surface of the fixed ring through supporting springs, and the two mounting plates are respectively fixedly connected to two adjacent sets of connecting ropes. Two symmetrical movable plates are slidably connected on the sides of the two mounting plates close to each other. A sliding groove adapted to the movable plate is provided on the inner surface of the fixed ring, and a fixed plate is fixed on one side of the two movable plates on the same side. A connecting spring is fixed on the side of the two fixed plates close to each other, and the two connecting springs are fixedly connected to the movable plate.

[0017] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0018] The concentrating component includes a concentrating tank fixed on the upper side of the water storage tank, a water pump three is fixed on the upper side of the concentrating tank, a concentrating tube is fixed in the inner cavity of the concentrating tank, an ultrafiltration membrane assembly is fixed in the inner cavity of the concentrating tube, an input pipe and an output pipe are fixed on both sides of the ultrafiltration membrane assembly, a spiral flow channel located inside the ultrafiltration membrane assembly is fixed between the input pipe and the output pipe, an output end of the water pump is communicated with the inner cavity of the input pipe through a delivery pipe, and an input end of the water pump is communicated with the inner cavity of the liquid storage tank through a delivery pipe.

[0019] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0020] A sewage discharge pipe communicating with the inner cavity of the concentrating tube is fixed on the side of the outer surface of the concentrating tube close to the input pipe, and a three-way electric-controlled valve is fixed on the end of the sewage discharge pipe away from the input pipe. One side of the horizontal part of the three-way electric-controlled valve is communicated with the inner cavity of the water storage tank through a delivery pipe, and the other side of the horizontal part of the three-way electric-controlled valve is communicated with the inner cavity of the sewage tank through a delivery pipe.

[0021] As a further description of the device for concentrating and purifying an antimicrobial peptide according to the above technology:

[0022] A water inlet pipe communicating with the inner cavity of the concentrator tube is fixed on one side of the outer surface of the concentrator tube close to the output pipe. The water inlet pipe is connected to the three output ends of the water pump, and the three input ends of the water pump are connected to the inner cavity of the water tank through a delivery pipe.

[0023] In summary, due to the use of the above-mentioned technology in the device for concentrating and purifying antimicrobial peptides, the beneficial effects of the present invention are:

[0024] 1. This device can perform tangential filtration on the broken cell fluid through the provided filtering components, thereby avoiding the deposition of macromolecular substances such as cell debris and lipids on the surface of the membrane, and can also backwash multiple filter components, thereby reducing the cell debris attached to the surface of the membrane in the filter component, avoiding the problem of frequent replacement due to clogging of the filter membrane, and at the same time making the filtered supernatant contain fewer impurities, reducing the impact on product quality; the broken cell fluid flows from the tee pipe 1 to the inner cavity of the filter component, and then flows to the next filter component through the connecting pipe, thereby forming a tangential filtration system, and the clean water in the inner cavity of the water tank is extracted by water pump 2 and transported to the tee pipe 2. At this time, the electric control valve 2 is closed, and the clean water in the tee pipe 2 is transported to the tangential filtration system composed of multiple filter components and connecting pipes, which is opposite to the flow path of the broken cell fluid, forming a backwash.

[0025] 2. This device can brush the filter screen through the cooperation between the filter assembly, the winding roller and the connecting rope, so as to remove impurities on the filter screen and the filter membrane and ensure the flux of the filter assembly. During the backwashing process, first, the connecting shaft is driven by the motor to rotate forward, and the filter screen rotates. Since the brush bar is fixed, the brush bar can clean the surface of the filter screen to loosen the cell debris on the filter screen. Then the motor is driven to reverse the connecting shaft. At this time, the filter screen no longer rotates, and the winding roller rotates. The two sets of connecting ropes are wound up to move the two mounting plates in the filter assembly toward each other, thereby reducing the liquid flow area between the two filter assemblies and increasing the liquid flow rate. The impurities on the filter screen and the filter membrane can be further washed away, further improving the backwashing effect.

[0026] 3. The device uses the concentrating components to make the antimicrobial peptide supernatant form turbulence in the spiral flow channel, destroy the static boundary layer on the membrane surface, reduce the local concentration accumulation of antimicrobial peptides on the membrane surface, reduce concentration polarization, avoid the decrease in filtration efficiency due to concentration gradient, and at the same time backwash the ultrafiltration membrane assembly to ensure the flux of the ultrafiltration membrane assembly; the fixed block transports the antimicrobial peptide supernatant in the liquid storage tank cavity to the input pipe cavity, and then forms turbulence through the acceleration of the spiral flow channel, and then passes through the ultrafiltration membrane assembly. Ultrafiltration makes the water flow to one side of the sewage pipe, and then discharged into the inner cavity of the water storage tank through the sewage pipe. The antimicrobial peptide concentrate is transported from the output pipe to the purification component for purification; water pump three extracts the clean water from the inner cavity of the water storage tank and transports it to the water inlet pipe, and then transports it to the inner cavity of the concentration tube through the three-way pipe to backwash the ultrafiltration membrane component. The backwashing waste liquid flows from the sewage pipe and the three-way electric control valve to the inner cavity of the sewage tank. By backwashing the ultrafiltration membrane component, the flux of the ultrafiltration membrane component can be restored and the filtration efficiency can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Shows the overall structure provided by the embodiment of the present invention Figure 1 ;

[0028] Figure 2 Shows the overall structure provided by the embodiment of the present invention Figure 2 ;

[0029] Figure 3 The structure diagram of the filter component provided by the embodiment of the present invention is shown. Figure 1 ;

[0030] Figure 4 The structure diagram of the filter component provided by the embodiment of the present invention is shown. Figure 2 ;

[0031] Figure 5 A cross-sectional view of the filter component structure provided according to an embodiment of the present invention is shown;

[0032] Figure 6 A schematic diagram of a tangential filtering structure of multiple filtering components provided according to an embodiment of the present invention is shown;

[0033] Figure 7 A cross-sectional view of the filter assembly structure provided according to an embodiment of the present invention is shown;

[0034] Figure 8 An exploded view of the filter assembly structure provided according to an embodiment of the present invention is shown;

[0035] Figure 9 A partial structural diagram of a filter assembly according to an embodiment of the present invention is shown;

[0036] Figure 10 A schematic diagram of tangential filtration of cell sap flow according to an embodiment of the present invention is shown;

[0037] Figure 11 Schematic diagram of the flow of antimicrobial peptide supernatant provided in accordance with an embodiment of the present invention is shown;

[0038] Figure 12 A schematic diagram of backwash water flow in a filter component provided by an embodiment of the present invention is shown;

[0039] Figure 13 A schematic diagram of a partial structure of a concentration component provided according to an embodiment of the present invention is shown;

[0040] Figure 14 A cross-sectional view of the structure of a concentrating component according to an embodiment of the present invention is shown;

[0041] Figure 15 A cross-sectional view of the structure of a concentrator tube and an ultrafiltration membrane assembly according to an embodiment of the present invention is shown;

[0042] Figure 16 A schematic diagram showing the flow of the antimicrobial peptide supernatant and the precipitated clean water provided by an embodiment of the present invention is shown;

[0043] Figure 17 A schematic diagram of backwashing water flow of an ultrafiltration membrane assembly provided by an embodiment of the present invention is shown;

[0044] Figure 18 A schematic diagram of the cooperation between the concentration component and the sewage tank provided in an embodiment of the present invention is shown.

[0045] Legend:

[0046] 10. Sewage tank; 11. Liquid storage tank; 12. Water storage tank; 13. Water pump 1;

[0047] 20. Filter element; 21. Filter box; 22. Water pump (2); 23. Fixing block; 24. Drive motor; 241. Connecting shaft; 242. Winding roller; 243. Connecting rope; 25. Tee (1); 26. Electric control valve (1);

[0048] 27. Filter assembly; 271. Fixing ring; 272. Filter membrane; 273. Filter screen; 274. Mounting plate; 275. Moving plate; 276. Brush strip; 277. Connecting spring; 278. Fixing plate;

[0049] 28. Two-way pipe; 29. Two electric control valves;

[0050] 30. Concentration component; 31. Concentration tank; 32. Water pump 3; 33. Inlet pipe; 34. Concentration pipe; 35. Ultrafiltration membrane assembly; 36. Spiral flow channel; 37. Output pipe; 38. Water inlet pipe; 39. Sewage and drainage pipe; 391. Three-way electric control valve;

[0051] 40. Purification components. DETAILED DESCRIPTION

[0052] The following, combined with the accompanying drawings, provides a clear and complete description of the technology in the embodiments of the present invention, an apparatus for concentrating and purifying antimicrobial peptides. Obviously, the described embodiments are only a portion of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0053] Example 1

[0054] like Figure 1 and Figure 2 As shown, a device for concentrating and purifying antimicrobial peptides includes a sewage tank 10 and a liquid storage tank 11. The sewage tank 10 is used to hold filtered impurities and backwash liquid. The liquid storage tank 11 is used to store filtered cell fluid. A filter component 20 is fixed on the upper side of the sewage tank 10. The filter component 20 is used to filter the broken cell fluid. A water storage tank 12 is fixed on one side of the liquid storage tank 11. The water storage tank 12 is used to store clean water for backwashing and clean water produced by ultrafiltration. A water pump 13 is fixed on the upper side of the liquid storage tank 11. A concentrating component 30 is fixed on the upper side of the water storage tank 12. The water pump 13 is used to extract the filtered cell fluid from the inner cavity of the liquid storage tank 11 and transport it to the inner cavity of the concentrating component 30 for ultrafiltration. A purification component 40 is installed on one side of the concentrating component 30.

[0055] The purification component 40 includes a chromatography system, which is all state-of-the-art and is used to purify the antimicrobial peptide concentrate. First, ion exchange chromatography (IEX) is used to adsorb the target peptide in a low-salt buffer based on the cationic properties of the antimicrobial peptide. Subsequently, the salt concentration (such as NaCl) is increased in a gradient to selectively elute and remove negatively charged impurities. Next, gel filtration chromatography (GFC) is used to separate, desalt or remove macromolecular aggregates based on molecular weight differences. Finally, high-resolution purification is achieved by reversed-phase high-performance liquid chromatography (RP-HPLC) on a hydrophobic C18 / C8 column using an acetonitrile / water gradient elution, and the target peak is collected by ultraviolet detection (214-280nm). The entire process takes into account differences in charge, molecular size, and hydrophobicity, ultimately obtaining a high-purity antimicrobial peptide. At the same time, the exposure time to the organic solvent must be controlled to maintain its biological activity.

[0056] Further, if Figure 3-Figure 6 As shown, the sewage tank 10 includes a filter box 21 and a water pump 22 fixedly connected to the filter box 21. The filter box 21 is fixed on the upper side of the sewage tank 10, and the water pump 22 is fixed on the upper side of the filter box 21. The lower side of the filter box 21 is connected to the inner cavity of the liquid storage tank 11 through multiple delivery pipes. The liquid in the inner cavity of the filter box 21 can flow into the inner cavity of the liquid storage tank 11 for storage. The inner cavity of the filter box 21 is fixed with multiple evenly distributed fixed blocks 23. The interval distance between two adjacent fixed blocks 23 is the same. The interior of the multiple fixed blocks 23 is inlaid with a filter component 27. The filter component 27 is fixed inside the fixed block 23 for filtering the broken cell fluid.

[0057] Among them, two adjacent filter assemblies 27 are connected by connecting pipes. The two adjacent connecting pipes are distributed up and down, so that a tangential flow loop is formed between the multiple filter assemblies 27. The two filter assemblies 27 on both sides are fixedly connected to the three-way pipe 1 25 and the two-way pipe 28. The three-way pipe 1 25 is used to connect to the disc centrifuge to transport the broken cell fluid to the inner cavity of the three-way pipe 1 25. A one-way valve is installed between the disc centrifuge and the three-way pipe 1 25 to prevent liquid backflow.

[0058] like Figure 5 and Figure 6 As shown, a tangential filtration flow loop is formed between multiple filter components 27 through the three-way pipe 25 and the connecting pipe. The circulation mode can reduce membrane clogging, and the multiple filter components 27 can be backwashed by the water pump 22, thereby reducing the frequency of membrane replacement and increasing the service life of the membrane. The tangential flow liquid flows parallel to the membrane surface to generate high shear force, flushing the membrane surface and reducing the deposition of macromolecular substances such as cell debris and lipids on the membrane surface.

[0059] Next, the vertical portion of the three-way pipe 1 25 is connected to an electric-controlled valve 1 26. The electric-controlled valve 1 26 is a type of valve in the prior art that can be opened and closed by electric control. One side of the electric-controlled valve 1 26 communicates with the inner cavity of the sewage tank 10 through a delivery pipe. The backwash liquid flows into the inner cavity of the sewage tank 10 through the electric-controlled valve 1 26. The horizontal portion of the two-way pipe 28 is fixed with an electric-controlled valve 29 on the side away from the filter assembly 27. One side of the electric-controlled valve 29 communicates with the inner cavity of the sewage tank 10 through a delivery pipe. The remaining impurities and cell fluid flow from the electric-controlled valve 29 and the delivery pipe into the inner cavity of the sewage tank 10.

[0060] The vertical portion of the second tee pipe 28 is connected to the output end of the second water pump 22 through a delivery pipe, and the input end of the second water pump 22 is connected to the inner cavity of the water storage tank 12 through the delivery pipe. The second electric control valve 29 is closed, and the clean water pumped by the second water pump 22 flows through the second tee pipe 28 to the inner cavity of the multiple filter components 27 to backwash the multiple filter components 27. At the same time, the first electric control valve 26 is opened to allow the backwash liquid to flow into the inner cavity of the sewage tank 10.

[0061] In addition, if Figure 5 and Figure 8 As shown, a driving motor 24 is fixed to one side of the filter box 21, and a connecting shaft 241 is fixed to the output end of the driving motor 24. A plurality of winding rollers 242 adapted to the filter assembly 27 are fixed to the outer surface of the connecting shaft 241. The plurality of winding rollers 242 are respectively located in the inner cavity of the filter assembly 27. Two groups of connecting ropes 243 are fixed to the outer surface of the plurality of winding rollers 242. The directions of the two groups of connecting ropes 243 are opposite. The two connecting ropes 243 in the same group are close to each other or away from each other, so that the connecting ropes 243 are evenly distributed.

[0062] like Figure 10 As shown, the broken cell fluid enters from the three-way pipe 25, and then flows into the multiple filter components 27 for tangential filtration, and the remaining cell waste fluid flows into the inner cavity of the sewage tank 10;

[0063] like Figure 11 As shown, the supernatant filtered out of the broken cell fluid flows from the inner cavity of the filter assembly 27 into the inner cavity of the filter box 21, and then flows into the inner cavity of the liquid storage box 11 through the delivery pipe on the lower side of the filter box 21;

[0064] like Figure 12 As shown, the water pump 22 draws clean water from the inner cavity of the water storage tank 12 and transports it to the vertical part of the tee pipe 28, and then flows through the tee pipe 28 to the inner cavities of multiple filter components 27 to backwash the filter components 27, thereby removing cell debris in the filter components 27, and then the backwashing liquid flows into the inner cavity of the sewage tank 10.

[0065] Further, if Figure 7-Figure 9As shown, the filter assembly 27 includes a fixed ring 271, which is embedded and fixed inside the fixed block 23. The inner surface of the fixed ring 271 is provided with two symmetrical filter membranes 272 and two symmetrical filter screens 273. The two fixed rings 271 are fixedly connected to each other and are used to filter impurities in the cell fluid. The two filter screens 273 are located inside the two filter membranes 272. The two filter screens 273 are connected to the connecting shaft 241 through a one-way bearing, allowing the filter screens 273 to rotate in one direction. The two filter screens 273 are used to filter cell debris in the cell fluid. The filter screens 273 are fixedly connected to the connecting shaft 241, allowing the filter screens 273 to rotate.

[0066] Two groups of symmetrical brush strips 276 are fixed to the inner surface of the fixing ring 271. There are multiple brush strips 276 in the same group. The brush strips 276 in the same group are in close contact with the surface of the filter screen 273 on the same side. The side of the brush strips 276 in close contact with the filter screen 273 is covered with bristles. When the filter screen 273 rotates, the bristles on the brush strips 276 can clean the cell debris on the filter screen 273 to prevent the cell debris from clogging the filter screen 273. In combination with backwashing, the cell debris on the filter screen 273 is cleaned, thereby improving the filtering effect of the filter screen 273.

[0067] Next, two symmetrical mounting plates 274 are fixed to the inner surface of the fixing ring 271 via support springs. The support springs are fixed to the inner surface of the fixing ring 271, and mounting plates 274 are fixed to the support springs. The two mounting plates 274 are respectively fixedly connected to two adjacent groups of connecting ropes 243. The two connecting ropes 243 in the same group are fixedly connected to the mounting plates 274. When the winding roller 242 rotates, the two connecting ropes 243 can pull the mounting plates 274 toward the center of the fixing ring 271. During the rotation of the winding roller 242, the filter screen 273 cannot rotate because it is connected to a one-way bearing and there is resistance at the connection between the filter screen 273 and the fixing ring 271.

[0068] Among them, the two mounting plates 274 are slidably connected to two symmetrical movable plates 275 on the side close to each other, and the inner surface of the fixed ring 271 is provided with a slide groove adapted to the movable plate 275. One side of the movable plate 275 slides in the slide groove and fits the side wall of the slide groove to prevent the liquid from flowing. A fixed plate 278 is fixed to one side of the two movable plates 275 on the same side, and a connecting spring 277 is fixed to the side of the two fixed plates 278 close to each other. The two connecting springs 277 are fixedly connected to the movable plates 275. In the process of the mounting plate 274 moving toward the center of the fixed ring 271, the two movable plates 275 move to both sides under the action of the connected connecting springs 277, so that the two movable plates 275 are always in contact with the side wall of the slide groove on the fixed ring 271;

[0069] An extension piece is fixed between the two movable plates 275 on the same side, so that the two movable plates 275 can be in the same straight line, thereby preventing the gap between the two movable plates 275 from hindering the flow of liquid.

[0070] By rotating the connecting shaft 241, the two sets of connecting ropes 243 are wound up, thereby pulling the mounting plates 274 on both sides to move, so that the cross-sectional area of the liquid flow between the two mounting plates 274 is reduced, thereby increasing the flow rate of the liquid. During the backwashing process, the cell debris and impurities on the filter screen 273 and the filter membrane 272 can be better washed away, thereby reducing the frequency of replacing the filter membrane due to blockage of the filter membrane 272, and further making the filter membrane 272 and the filter screen 273 cleaner, making the filtered supernatant cleaner, thereby improving the quality of the antimicrobial peptide.

[0071] Example 2

[0072] This embodiment further defines the concentrating component 30 based on the first embodiment, so as to increase the shear force through the spiral flow channel 36 and thus avoid the decrease in flux due to adsorption of impurities on the surface of the ultrafiltration membrane.

[0073] Specifically, such as Figure 13 — Figure 15 As shown, the concentrating component 30 includes a concentrating tank 31 fixed to the upper side of the water storage tank 12, a water pump 32 is fixed to the upper side of the concentrating tank 31, a concentrating tube 34 is fixed to the inner cavity of the concentrating tube 34, an ultrafiltration membrane assembly 35 is fixed to the inner cavity of the concentrating tube 34, the ultrafiltration membrane assembly 35 includes two mounting circular plates and a plurality of ultrafiltration membrane wires, an input tube 33 and an output tube 37 are fixed on both sides of the ultrafiltration membrane assembly 35, the input tube 33 and the output tube 37 are directly connected to the ultrafiltration membrane assembly 35, and a spiral flow channel 36 located inside the ultrafiltration membrane assembly 35 is fixed between the input tube 33 and the output tube 37. The spiral flow channel 36 is spiral, which can make the liquid entering the ultrafiltration membrane assembly 35 from the input tube 33 spiral, thereby enhancing the shear force of the liquid, and the high-speed fluid forms turbulence in the spiral structure, destroying the static boundary layer on the membrane surface, reducing the local concentration accumulation of antimicrobial peptides on the membrane surface, reducing concentration polarization, and avoiding the decrease in filtration efficiency due to concentration gradient;

[0074] The output end of the water pump 13 is connected to the inner cavity of the input pipe 33 through a delivery pipe, and the input end of the water pump 13 is connected to the inner cavity of the liquid storage tank 11 through a delivery pipe. The water pump 13 delivers the filtered supernatant in the inner cavity of the liquid storage tank 11 to the inner cavity of the input pipe 33 for concentration;

[0075] Next, a sewage drain pipe 39 is fixed to the outer surface of the concentrating tube 34 on the side close to the inlet pipe 33. The drain pipe 39 is connected to the inner cavity of the concentrating tube 34. The ultrafiltration membrane assembly 35 is located in the inner cavity of the concentrating tube 34. There is a certain space between the ultrafiltration membrane assembly 35 and the two side walls of the inner cavity of the two concentrating tubes 34. The sewage drain pipe 39 is located in the space between the ultrafiltration membrane assembly 35 and the concentrating tube 34 on the side close to the inlet pipe 33. A three-way electric control valve 391 is fixed to the end of the sewage drain pipe 39 away from the inlet pipe 33. The three-way electric control valve 391 is three-way and can control the direction of liquid flow.

[0076] like Figure 18 As shown, one side of the horizontal portion of the three-way electric control valve 391 is connected to the inner cavity of the water storage tank 12 through a delivery pipe. The clean water produced during the ultrafiltration process flows through the inner cavity of the water storage tank 12 through the sewage discharge pipe 39. The other side of the horizontal portion of the three-way electric control valve 391 is connected to the inner cavity of the sewage tank 10 through a delivery pipe. The waste liquid produced during the backwash process flows into the inner cavity of the sewage tank 10 through the sewage discharge pipe 39.

[0077] like Figure 16 As shown, during the ultrafiltration process, liquid enters from the input pipe 33 and flows through the spiral flow channel 36, causing the clean water and impurities to move tangentially along the membrane fibers. The clean water flows toward the side of the input pipe 33 and is transported to the inner cavity of the water storage tank 12 through the sewage discharge pipe 39 and the delivery pipe. The concentrated liquid is transported from the output pipe 37 to the purification component 40 for purification.

[0078] In addition, a water inlet pipe 38 is fixed to the outer surface of the concentrating tube 34 on one side near the output pipe 37. The water inlet pipe 38 is located in the space between the ultrafiltration membrane assembly 35 and the concentrating tube 34 on the side of the output pipe 37. The water inlet pipe 38 is connected to the output end of the water pump 32. The input end of the water pump 32 is connected to the inner cavity of the water storage tank 12 through a delivery pipe.

[0079] like Figure 17 As shown, water pump three 32 can extract clean water from the inner cavity of the water storage tank 12 to backwash the ultrafiltration membrane assembly 35, thereby avoiding the problem of flux reduction due to membrane clogging.

[0080] It should be noted that the water pump 13, drive motor 24, three-way pipe 1 25, electric control valve 1 26, three-way pipe 2 28, electric control valve 2 29, water pump 3 32, ultrafiltration membrane assembly 35, three-way electric control valve 391 and purification assembly 40 in the present invention are all existing technologies, and their installation methods and control methods are also conventional designs, which will not be elaborated in detail in the present invention.

[0081] Working principle of the present invention: This device is a device for concentrating and purifying antimicrobial peptides. The provided filter component 20 can perform tangential filtration on the broken cell fluid, thereby preventing the deposition of macromolecular substances such as cell debris and lipids on the surface of the membrane. It can also backwash multiple filter components 27, thereby reducing cell debris attached to the surface of the membrane in the filter component 27, avoiding the problem of frequent replacement due to clogging of the filter membrane 272, and at the same time, making the impurities in the filtered supernatant less, reducing the impact on product quality.

[0082] Specifically, such as Figure 10 As shown, the cell fluid after being broken by the disc centrifuge enters the inner cavity of the three-way pipe 25. At this time, the electric control valve 26 is in a closed state. The broken cell fluid flows from the three-way pipe 25 to the inner cavity of the filter component 27, and then flows to the next filter component 27 through the connecting pipe, thereby forming a tangential filtration system. During the tangential flow of the broken cell fluid, the tangential flow liquid flows parallel to the membrane surface to generate high shear force, flushing the membrane surface and reducing the deposition of macromolecules such as cell debris and lipids on the membrane surface. Finally, the filtered cell fluid flows from the horizontal part of the three-way pipe 28 to the electric control valve 29, and then flows from the delivery pipe connected to the electric control valve 29 to the inner cavity of the sewage tank 10;

[0083] Then, if Figure 11 As shown, during the flow of the broken cell fluid, the cell fluid enters the inner cavity of the filter assembly 27. First, the filter meshes 273 on both sides filter out the macromolecular impurities, and then the filter membrane 272 blocks the small molecular impurities. Finally, the antimicrobial peptide supernatant flows from the inner cavity of the filter assembly 27 to the inner cavity of the filter box 21, and then flows into the inner cavity of the liquid storage tank 11 through the delivery pipe on the lower side of the filter box 21.

[0084] Backwashing of tangential filtration systems, such as Figure 12 As shown, the clean water in the inner cavity of the water storage tank 12 is extracted by the water pump 22 and transported to the tee pipe 2 28. At this time, the electric control valve 29 is closed, and the clean water in the tee pipe 2 28 is transported to the tangential filtration system composed of multiple filter components 27 and connecting pipes. The flow path is opposite to that of the broken cell liquid, forming a backwash, which can bring out impurities in the inner cavity of the filter component 27, and then flow from the vertical part of the tee pipe 1 25 to the electric control valve 1 26, and then flow from the electric control valve 1 26 delivery pipe into the inner cavity of the sewage tank 10. Through the backwashing provided, the flux of the filter membrane 272 and the filter screen 273 can be restored, thereby suppressing membrane pollution and extending the life of the membrane. Moreover, the flushing can be performed without dismantling, which can reduce the downtime and thus improve the filtration efficiency.

[0085] In addition, during the backwashing process, first, the motor 24 is driven to rotate the connecting shaft 241 forward, thereby rotating the filter screen 273. Since the brush bar 276 is fixed, the brush bar 276 can clean the surface of the filter screen 273 to loosen the cell debris on the filter screen 273. Then the motor 24 is driven to reverse the connecting shaft 241. At this time, the filter screen 273 no longer rotates, and the winding roller 242 rotates. The two sets of connecting ropes 243 are wound up, so that the two mounting plates 274 in the filter assembly 27 move toward each other, thereby reducing the liquid flow area between the two filter assemblies 27 and increasing the speed of liquid flow. Impurities on the filter screen 273 and the filter membrane 272 can be further washed away, further improving the backwashing effect.

[0086] During the concentration of the antimicrobial peptide supernatant, the concentrating component 30 is provided to form turbulent flow in the spiral flow channel 36, thereby destroying the static boundary layer on the membrane surface, reducing the local concentration accumulation of the antimicrobial peptide on the membrane surface, reducing concentration polarization, and avoiding the decrease in filtration efficiency due to concentration gradient;

[0087] Specifically, such as Figure 16 As shown, the fixed block 23 transports the antimicrobial peptide supernatant in the inner cavity of the liquid storage tank 11 to the inner cavity of the input pipe 33, and then it is accelerated by the spiral flow channel 36 to form turbulent flow. Then, it is ultrafiltered by the ultrafiltration membrane assembly 35, causing the water to flow to the side of the sewage drainage pipe 39. Then, it is discharged into the inner cavity of the water storage tank 12 through the sewage drainage pipe 39. The antimicrobial peptide concentrate is transported from the output pipe 37 to the purification assembly 40 for purification.

[0088] Then, if Figure 17 As shown, water pump three 32 draws clean water from the inner cavity of the water storage tank 12 and transports it to the water inlet pipe 38, and then transports it to the inner cavity of the concentration tube 34 through the three-way pipe 28 to backwash the ultrafiltration membrane assembly 35. The backwashed waste liquid flows from the sewage pipe 39 and the three-way electric control valve 391 to the inner cavity of the sewage tank 10. By backwashing the ultrafiltration membrane assembly 35, the flux of the ultrafiltration membrane assembly 35 can be restored, thereby improving the filtration efficiency.

[0089] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art, within the technical scope disclosed by the present invention, who can make equivalent replacements or changes to the antimicrobial peptide concentration and purification device and its inventive concept according to the technology of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A device for concentrating and purifying antimicrobial peptides, comprising a sewage tank (10) and a liquid storage tank (11), characterized in that: A filter component (20) is fixed on the upper side of the sewage tank (10), a water tank (12) is fixed on one side of the liquid storage tank (11), a water pump (13) is fixed on the upper side of the liquid storage tank (11), a concentration component (30) is fixed on the upper side of the water storage tank (12), and a purification component (40) is installed on one side of the concentration component (30); The sewage tank (10) comprises a filter box (21) and a second water pump (22) fixedly connected to the filter box (21); the lower side of the filter box (21) is communicated with the inner cavity of the liquid storage box (11) through a plurality of delivery pipes; a plurality of evenly distributed fixed blocks (23) are fixed to the inner cavity of the filter box (21); a plurality of the fixed blocks (23) are embedded with filter assemblies (27); two adjacent filter assemblies (27) are connected by a connecting pipe; the two filter assemblies (27) located on both sides are fixedly connected to a first tee pipe (25) and a second tee pipe (28) respectively; A tangential filtering flow loop is formed between the plurality of filter assemblies (27) through the three-way pipe (25) and the connecting pipe, and the plurality of filter assemblies (27) can be backwashed by extracting clean water from the water storage tank (12) through the water pump (22).

2. The device for concentrating and purifying antimicrobial peptides according to claim 1, characterized in that: The vertical portion of the three-way pipe (25) is connected to an electric control valve (26), one side of which is communicated with the inner cavity of the sewage tank (10) through a delivery pipe. The horizontal portion of the two-way pipe (28) is fixed with an electric control valve (29) on a side away from the filter assembly (27), one side of which is communicated with the inner cavity of the sewage tank (10) through a delivery pipe. The vertical portion of the two-way pipe (28) is connected to the output end of the two-way water pump (22) through a delivery pipe, and the input end of the two-way water pump (22) is communicated with the inner cavity of the water storage tank (12) through a delivery pipe.

3. The device for concentrating and purifying antimicrobial peptides according to claim 1, characterized in that: A driving motor (24) is fixed to one side of the filter box (21), a connecting shaft (241) is fixed to the output end of the driving motor (24), a plurality of winding rollers (242) adapted to the filter assembly (27) are fixed to the outer surface of the connecting shaft (241), and two groups of connecting ropes (243) are fixed to the outer surfaces of the plurality of winding rollers (242).

4. The device for concentrating and purifying antimicrobial peptides according to claim 1, characterized in that: The filter assembly (27) comprises a fixed ring (271), the inner surface of which is provided with two symmetrical filter membranes (272) and two symmetrical filter screens (273), the two filter screens (273) being located inside the two filter membranes (272), the two filter screens (273) being fixedly connected to the connecting shaft (241), and the inner surface of the fixed ring (271) being fixed with two groups of symmetrical brush strips (276), the brush strips (276) of the same group being in close contact with the surface of the filter screen (273) on the same side.

5. The device for concentrating and purifying antimicrobial peptides according to claim 4, characterized in that: Two symmetrical mounting plates (274) are fixed on the inner surface of the fixed ring (271) through supporting springs. The two mounting plates (274) are respectively fixedly connected to two adjacent groups of connecting ropes (243). Two symmetrical movable plates (275) are slidably connected to the sides of the two mounting plates (274) adjacent to each other. A sliding groove adapted to the movable plates (275) is provided on the inner surface of the fixed ring (271). A fixed plate (278) is fixed on one side of the two movable plates (275) on the same side. A connecting spring (277) is fixed on the sides of the two fixed plates (278) adjacent to each other. The two connecting springs (277) are fixedly connected to the movable plates (275).

6. The device for concentrating and purifying antimicrobial peptides according to claim 1, characterized in that: The concentrating component (30) includes a concentrating tank (31) fixed on the upper side of the water storage tank (12), a water pump three (32) is fixed on the upper side of the concentrating tank (31), a concentrating tube (34) is fixed in the inner cavity of the concentrating tank (31), an ultrafiltration membrane assembly (35) is fixed in the inner cavity of the concentrating tube (34), an input tube (33) and an output tube (37) are fixed on both sides of the ultrafiltration membrane assembly (35), a spiral flow channel (36) located inside the ultrafiltration membrane assembly (35) is fixed between the input tube (33) and the output tube (37), the output end of the water pump one (13) is communicated with the inner cavity of the input tube (33) through a delivery tube, and the input end of the water pump one (13) is communicated with the inner cavity of the liquid storage tank (11) through a delivery tube.

7. The device for concentrating and purifying antimicrobial peptides according to claim 6, characterized in that: A sewage discharge pipe (39) communicating with the inner cavity of the concentrating pipe (34) is fixed on the side of the outer surface of the concentrating pipe (34) close to the input pipe (33), and a three-way electric control valve (391) is fixed on the end of the sewage discharge pipe (39) away from the input pipe (33). One side of the horizontal portion of the three-way electric control valve (391) is communicated with the inner cavity of the water storage tank (12) through a delivery pipe, and the other side of the horizontal portion of the three-way electric control valve (391) is communicated with the inner cavity of the sewage tank (10) through a delivery pipe.

8. The device for concentrating and purifying antimicrobial peptides according to claim 7, characterized in that: A water inlet pipe (38) communicating with the inner cavity of the concentrating pipe (34) is fixed on one side of the outer surface of the concentrating pipe (34) close to the output pipe (37). The water inlet pipe (38) is connected to the output end of the water pump three (32). The input end of the water pump three (32) is communicated with the inner cavity of the water storage tank (12) through a delivery pipe.

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