Teaching auxiliary device for paclitaxel drug-resistant breast cancer cell exosome separation

Abstract

The utility model relates to the technical field of medical teaching, and discloses a teaching auxiliary device for paclitaxel drug-resistant breast cancer cell exosome separation, which comprises L-shaped supporting legs, an operation table, a supporting vertical plate A and a supporting vertical plate B, a supporting vertical plate A is mounted at one end of the top of the operating table through screws, a horizontal top plate is welded to one side of the top end of the supporting vertical plate A, a filter-pressing electric cylinder is mounted at the bottom of the horizontal top plate through a mounting seat, and a piston plate is mounted on an output shaft of the filter-pressing electric cylinder through screws; according to the teaching auxiliary device for separating the taxol drug-resistant breast cancer cell exosome, cell debris and large-particle substances can be conveniently removed in advance and rapidly cleaned, the cell exosome and tissue fluid are automatically collected, subsequent centrifugal operation is facilitated, a sample is heated to a proper temperature, and the subsequent cell exosome separation effect is improved.

Description

Technical Field

[0001] This utility model relates to the field of medical teaching technology, and in particular to a teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells. Background Technology

[0002] The isolation of exosomes from paclitaxel-resistant breast cancer cells is a complex and delicate process. Exosomes are extracellular vesicles secreted by all living cells, with a diameter of 30-150 nm, containing the genetic information of their originating cells. After being released into the extracellular environment, these vesicles exist uniformly and stably in the circulatory system and are widely present in physiological or pathological fluids, such as plasma, synovial fluid, saliva, sputum, cerebrospinal fluid, lymph, and ascites. Specialized teaching aids are required when learning the exosome isolation experiment for paclitaxel-resistant breast cancer cells.

[0003] Previous teaching aids for isolating exosomes from paclitaxel-resistant breast cancer cells had the following drawbacks: 1. They were inconvenient for pre-removing cell debris and large particles and for rapid cleaning; 2. They could not automatically collect exosomes and tissue fluid for subsequent centrifugation; and 3. They could not heat the sample to a suitable temperature to improve the subsequent exosome isolation effect. Therefore, those skilled in the art have provided a teaching aid for isolating exosomes from paclitaxel-resistant breast cancer cells to solve the problems mentioned in the background art. Utility Model Content

[0004] The main purpose of this invention is to provide a teaching aid for isolating exosomes from paclitaxel-resistant breast cancer cells, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solutions:

[0006] A teaching aid for isolating exosomes from paclitaxel-resistant breast cancer cells, comprising an L-shaped support leg, an operating table, support plate A, and support plate B;

[0007] A support plate A is screwed onto one end of the top of the operating table. A horizontal top plate is welded to one side of the top of the support plate A. A filter press cylinder is mounted on the bottom of the horizontal top plate via a mounting seat, and a piston plate is screwed onto the output shaft of the filter press cylinder. A collection cylinder is connected to one side of the support plate A via a connecting plate. A temperature controller is mounted on one side of the collection cylinder via a mounting side plate. A ceramic heater is mounted on one end of the temperature controller via a mounting hole. A ceramic inner liner is fitted inside the collection cylinder, and a filter press cylinder is fitted inside the ceramic inner liner. A filter press membrane is screwed onto the bottom of the filter press cylinder. A limiting ring is fitted onto the top of the filter press cylinder and rests on the top of the ceramic inner liner. A support plate B is screwed onto the other end of the top of the operating table, and a camera is mounted on one side of the top of the support plate B via a rotating seat. A centrifuge is located on the top of the operating table, between the support plates A and B.

[0008] As a further embodiment of this utility model: an electric cylinder controller is installed at the bottom of the horizontal top plate and at one end of the filter press electric cylinder by screws. The output end of the electric cylinder controller is electrically connected to the input end of the filter press electric cylinder by wires. The electric cylinder controller automatically controls the output stroke of the filter press electric cylinder, thereby automatically raising and lowering the piston plate to a certain height.

[0009] As a further improvement of this utility model, rubber support blocks are installed at equal intervals on the outer side of the bottom end of the filter cylinder by screws.

[0010] As a further embodiment of this utility model: the bottom of the collecting cylinder is connected to an arc-shaped material guide port and a PP limiting sleeve is fitted at the bottom of the arc-shaped material guide port, and a centrifuge test tube is fitted inside the PP limiting sleeve.

[0011] As a further improvement of this utility model: a test tube rack is provided between the centrifuge and the supporting vertical plate B, and L-shaped support legs are installed on both sides of the bottom of the operating table by screws.

[0012] As a further embodiment of this invention: the detection end of the temperature controller and the heating end of the ceramic heater are both located inside the ceramic liner. The output end of the temperature controller is connected to the input end of the ceramic heater through a wire. The temperature controller automatically controls the ceramic heater to maintain constant heating inside the ceramic liner, thereby increasing the temperature of the sample inside the filter press cylinder.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. The output stroke of the filter press cylinder is automatically controlled by the electric cylinder controller, thereby automatically raising the piston plate. The paclitaxel-resistant breast cancer cell sample solution is pre-placed in the filter press cylinder. The external power supply of the temperature controller and ceramic heater is turned on. The temperature controller automatically controls the ceramic heater to keep the ceramic inner liner heated, thereby increasing the temperature of the sample in the filter press cylinder, increasing cell activity, increasing the intermolecular movement rate, and facilitating the improvement of subsequent centrifugation effect.

[0015] 2. Next, the piston plate is pressed into the filter cylinder using a filter cylinder. Using a filter membrane, rapid filtration of paclitaxel-resistant breast cancer cell samples is achieved, allowing exosomes and tissue fluid to enter the ceramic inner liner and be automatically collected in a centrifuge tube through an arc-shaped feed inlet. Cell debris and large particles remain in the filter cylinder, which is then removed for cleaning. The exosome precipitate is then collected by high-speed centrifugation. A camera is used to record the process of separating cancer cell exosomes, facilitating playback for review and correction, and providing valuable teaching video material. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to this utility model.

[0017] Figure 2 This is a schematic diagram of the collection cylinder and filter press of the teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to this utility model.

[0018] Figure 3 This is a schematic diagram of the PP limiting sleeve structure of the teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to this utility model.

[0019] Figure 4 This is a schematic diagram of the pressure filter membrane structure of the teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to this utility model.

[0020] In the diagram: 1. L-shaped support leg; 2. Operating table; 3. Centrifuge tube; 4. Arc-shaped feed inlet; 5. Collection cylinder; 6. Limiting ring; 7. Filter press cylinder; 8. Supporting vertical plate A; 9. Electric cylinder controller; 10. Horizontal top plate; 11. Filter press electric cylinder; 12. Piston plate; 13. Camera; 14. Supporting vertical plate B; 15. Centrifuge; 16. Test tube rack; 17. Rubber support block; 18. Ceramic inner liner; 19. Temperature controller; 20. Ceramic heater; 21. PP limiting sleeve; 22. Filter press membrane. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-4 In this embodiment of the invention, the teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells includes an L-shaped support leg 1, an operating table 2, a support vertical plate A8, and a support vertical plate B14.

[0023] A support plate A8 is screwed onto one end of the top of the operating table 2. A horizontal top plate 10 is welded to one side of the top of the support plate A8. A filter press cylinder 11 is mounted on the bottom of the horizontal top plate 10 via a mounting base, and a piston plate 12 is screwed onto the output shaft of the filter press cylinder 11. A collection cylinder 5 is connected to one side of the support plate A8 via a connecting plate. A temperature controller 19 is mounted on one side of the collection cylinder 5 via a mounting side plate. A ceramic heater 20 is mounted on one end of the temperature controller 19 via a mounting hole. 5. A ceramic inner liner 18 is provided inside the ceramic inner liner 18, and a filter press 7 is provided inside the ceramic inner liner 18. A filter press membrane 22 is installed at the bottom of the filter press 7 by screws. A limiting ring 6 is provided at the top of the filter press 7 and is attached to the top of the ceramic inner liner 18. A support vertical plate B14 is installed at the other end of the top of the operating table 2 by screws. A camera 13 is installed on one side of the top of the support vertical plate B14 by a rotating seat. A centrifuge 15 is provided at the top of the operating table 2 and between the support vertical plate A8 and the support vertical plate B14.

[0024] The bottom of the horizontal top plate 10 and one end of the filter press cylinder 11 are equipped with an electric cylinder controller 9 by screws. The output end of the electric cylinder controller 9 is electrically connected to the input end of the filter press cylinder 11 by wires. The electric cylinder controller 9 automatically controls the output stroke of the filter press cylinder 11, thereby automatically raising and lowering the piston plate 12 to a certain height.

[0025] Among them, rubber support blocks 17 are installed at equal intervals on the outer side of the bottom end of the filter press cylinder 7 by screws; the rubber support blocks 17 are used to limit the position of the filter press cylinder 7 so that the filter press cylinder 7 is centered in the ceramic inner liner.

[0026] The bottom of the collecting cylinder 5 is connected to an arc-shaped material guide port 4, and a PP limiting sleeve 21 is fitted at the bottom of the arc-shaped material guide port 4. A centrifuge tube 3 is fitted inside the PP limiting sleeve 21. The PP limiting sleeve 21 has high elasticity and good toughness, making it easy to fit the centrifuge tube 3 inside the PP limiting sleeve 21 to receive material.

[0027] A test tube rack 16 is provided between the centrifuge 15 and the support plate B14, and L-shaped support legs 1 are installed on both sides of the bottom of the operating table 2 by screws; the test tube rack 16 is used to facilitate the placement of centrifuge test tubes 3, and the L-shaped support legs 1 provide stable support for the operating table 2.

[0028] The detection end of the temperature controller 19 and the heating end of the ceramic heater 20 are both located inside the ceramic inner liner 18. The output end of the temperature controller 19 is connected to the input end of the ceramic heater 20 through a wire. The temperature controller 19 automatically controls the ceramic heater 20 to keep the ceramic inner liner 18 heated, thereby increasing the temperature of the sample in the filter press 7.

[0029] The working principle of this invention is as follows: The electric cylinder controller 9 automatically controls the output stroke of the filter press cylinder 11, thereby automatically raising the piston plate 12. Paclitaxel-resistant breast cancer cell sample solution is pre-placed inside the filter press cylinder 7. The external power supply to the temperature controller 19 and ceramic heater 20 is connected. The temperature controller 19 automatically controls the ceramic heater 20 to maintain constant heating inside the ceramic inner liner 18, thereby increasing the temperature of the sample inside the filter press cylinder 7, increasing cell activity, and increasing the rate of intermolecular movement, which facilitates improved subsequent centrifugation. Then, the filter press cylinder 11 is used to... The stopper plate 12 is pressed into the filter press cylinder 7. Using the filter press membrane 22, the sample fluid of paclitaxel-resistant breast cancer cells is rapidly filtered, allowing cell exosomes and tissue fluid to enter the ceramic inner liner 18. The fluid is then automatically collected in the centrifuge tube 3 through the arc-shaped feed port 4. Cell debris and large particles remain in the filter press cylinder 7. The filter press cylinder 7 is removed for easy cleaning. The exosome precipitate is then collected by high-speed centrifugation using a centrifuge 15. The camera 13 is used to record the teaching process of separating cancer cell exosomes, which can be easily reviewed and supplemented, and can also be used as teaching video material.

[0030] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A teaching aid for isolating exosomes from paclitaxel-resistant breast cancer cells, comprising an L-shaped support leg (1), an operating table (2), a support vertical plate A (8), and a support vertical plate B (14); Its characteristics are: A support plate A (8) is screwed onto one end of the top of the operating table (2). A horizontal top plate (10) is welded to one side of the top of the support plate A (8). A filter press cylinder (11) is mounted on the bottom of the horizontal top plate (10) via a mounting base, and a piston plate (12) is screwed onto the output shaft of the filter press cylinder (11). A collection cylinder (5) is connected to one side of the support plate A (8) via a connecting plate. A temperature controller (19) is mounted on one side of the collection cylinder (5) via a mounting side plate. A ceramic heater (20) is mounted on one end of the temperature controller (19) via a mounting hole. The collection cylinder ( 5) An inner ceramic liner (18) is provided inside the inner ceramic liner (18) and a filter press (7) is provided inside the inner ceramic liner (18). A filter press membrane (22) is installed at the bottom of the filter press (7) by screws. A limiting ring (6) is provided at the top of the filter press (7) and the limiting ring (6) is placed on the top of the inner ceramic liner (18). A support plate B (14) is installed at the other end of the top of the operating table (2) by screws. A camera (13) is installed on one side of the top of the support plate B (14) by a rotating seat. A centrifuge (15) is provided at the top of the operating table (2) and between the support plate A (8) and the support plate B (14).

2. The teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to claim 1, characterized in that: An electric cylinder controller (9) is installed at the bottom of the horizontal top plate (10) and at one end of the filter press electric cylinder (11) by screws. The output end of the electric cylinder controller (9) and the input end of the filter press electric cylinder (11) are electrically connected by wires.

3. The teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to claim 1, characterized in that: Rubber support blocks (17) are installed at equal intervals on the outer side of the bottom end of the filter press cylinder (7) by screws.

4. The teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to claim 1, characterized in that: The bottom of the collecting cylinder (5) is connected to an arc-shaped feed inlet (4), and a PP limiting sleeve (21) is fitted at the bottom of the arc-shaped feed inlet (4). A centrifuge tube (3) is fitted inside the PP limiting sleeve (21).

5. The teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to claim 1, characterized in that: A test tube rack (16) is provided between the centrifuge (15) and the support plate B (14), and L-shaped support legs (1) are installed on both sides of the bottom of the operating table (2) by screws.

6. The teaching aid device for isolating exosomes from paclitaxel-resistant breast cancer cells according to claim 1, characterized in that: The detection end of the thermostat (19) and the heating end of the ceramic heater (20) are both located inside the ceramic liner (18), and the output end of the thermostat (19) is connected to the input end of the ceramic heater (20) through a wire.

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