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Method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish

A bioactive peptide and protein powder technology, applied in animal feed, animal feed, applications, etc., can solve the problems of long time for enzymatic hydrolysis of small peptides, high cost of biochemical enzymes, and difficulty in large-scale production, and achieves enhanced practicability, The effect of reducing production cost and low production cost

Inactive Publication Date: 2010-06-16
陈忆凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for the preparation of bioactive peptides, the most common ones are enzymatic hydrolysis and microbial methods, but due to the high cost of external biochemical enzymes in the enzymatic hydrolysis method, the preparation of small peptides by the microbiological method takes too long to enzymatically hydrolyze, and the cost is high, so it is difficult to achieve industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. A method for preparing marine low-value miscellaneous fish endogenous enzymatically hydrolyzed bioactive peptide protein powder, the steps of which are as follows:

[0018] (1) Raw material preparation: select marine low-value small trash fish as raw material; measure the endogenous enzyme activity of the raw material, and for the raw material with endogenous enzyme activity greater than 200ug / g, use the endogenous enzyme in step (3) when performing enzymatic hydrolysis Enzymatic hydrolysis process; for raw materials with endogenous enzyme activity less than 200ug / g, the exogenous enzyme enzymatic hydrolysis process in step (3) is used for enzymolysis;

[0019] (2) One-time pulverization: put the raw materials into the pulverizer, add water to the raw materials according to the weight ratio of raw materials and water 1: 1 for soaking and thawing treatment, until the raw materials are completely dispersed without frozen blocks; start the pulverizer to pulveriz...

Embodiment 2

[0024] Example 2. A method for preparing marine low-value miscellaneous fish endogenous enzymatically hydrolyzed bioactive peptide protein powder, the steps of which are as follows:

[0025] (1) Raw material preparation: choose aquatic product processing waste as raw material; measure the endogenous enzyme activity of raw material, for the raw material with endogenous enzyme activity greater than 200ug / g, use the endogenous enzyme enzyme in step (3) when carrying out enzymatic hydrolysis hydrolysis process; for endogenous enzyme activity less than 200ug / g raw material, adopt the exogenous enzyme enzymolysis process in step (3) when carrying out enzymolysis;

[0026] (2) One-time pulverization: put the raw materials into the pulverizer, add water to the raw materials according to the weight ratio of raw materials and water 1:2 to soak and thaw, until the raw materials are completely scattered without frozen blocks; start the pulverizer to pulverize, pulverize to 100% of the ra...

Embodiment 3

[0031] Example 3. A method for preparing marine low-value miscellaneous fish endogenous enzymatically hydrolyzed bioactive peptide protein powder, the steps of which are as follows:

[0032](1) Raw material preparation: select marine low-value small miscellaneous fish and aquatic product processing waste as raw materials; measure the endogenous enzyme activity of the raw material, for the raw material with endogenous enzyme activity greater than 200ug / g, adopt the step (3 ) in the endogenous enzyme enzymatic hydrolysis process; for endogenous enzyme activity less than 200ug / g raw material, adopt the exogenous enzyme enzymatic hydrolysis process in step (3) when carrying out enzymatic hydrolysis;

[0033] (2) One-time pulverization: put the raw material into the pulverizer, add water to the raw material according to the weight ratio of raw material and water 1: 1.5 for soaking and thawing treatment, until the raw material is completely scattered without frozen blocks; start the...

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PUM

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Abstract

The invention discloses a method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish, which is characterized by comprising steps of raw material preparation, primary crushing, enzymolysis, small peptide liquid preparation, mixing and drying, post treatment and the like. During enzymolysis, enzymolysis process parameters are selected according to the endogenous enzyme activity measured from the raw materials; and during mixing and drying, peeled bean pulp powder is used as a carrier to prepare a bioactive protein material. The method of the invention performs process improvement based on the enzymolysis of the primary exogenous proteinase preparation, solves the difficult problems of high production cost, unstable exogenous enzyme activity and non-thorough enzymolysis in the endogenous enzymolysis process, and is more suitable for industrialized production; and the prepared bioactive protein powder has the sense, flavor and cultivation effects superior to those of the fish powder, and is a good fish powder substitute.

Description

technical field [0001] The invention relates to a preparation method of a feed additive, in particular to a preparation method of endogenous enzymatically hydrolyzed bioactive peptide protein powder of marine low-value trash fish. Background technique [0002] Small peptides are intermediate products in the process of degrading protein into amino acids in animals. They are important nutrients for animals and one of the important ways of protein nutrition absorption and metabolism except amino acids. Small peptides and free amino acids have independent absorption mechanisms. They do not interfere with each other, and help the absorption of amino acids and speed up protein synthesis. Adding peptide products to animal diets can increase the utilization rate of amino acids, reduce the occurrence of diseases, give full play to its production performance and improve economic benefits. [0003] Bioactive peptides have been highly praised by the pharmaceutical, food and feed indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10A23K1/16
Inventor 陈忆凤朱勤
Owner 陈忆凤
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