Preparation method of fishskin/scale collagen peptide

A fish scale collagen and fish skin technology is applied in the field of food biology to achieve the effects of simple process, increased accessibility and easy operation

Active Publication Date: 2018-11-23
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for preparing fish skin and scale collagen peptides, which ca

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, a kind of preparation method of fish skin / scale collagen peptide, carries out following steps successively:

[0034] (1) Steam explosion treatment of fish skin and fish scales:

[0035] Use the fish skin and scales of high-quality tilapia (that is, fish skin with fish scales) as raw material, wash it, remove impurities, put it into the explosion chamber of the steam explosion machine, steam explosion treatment at 0.6MPa for 1min, and take it out after pressure relief; Raw materials after steam explosion;

[0036] (2), water extraction:

[0037] Add 5 mass times of water to the raw material after steam explosion, mix evenly, extract at 50°C for 2 hours, and filter to obtain fish skin and scale extract;

[0038] (3), heat preservation enzymatic hydrolysis:

[0039] First adjust the pH of the extract to 8, then add Alcalase alkaline protease (enzyme activity is 3.0AU / g) sold by Novozymes, the amount of alkaline protease is 0.1% of the raw material weight a...

Embodiment 2

[0048] Embodiment 2, the preparation method of fish skin / scale collagen peptide, carries out following steps successively:

[0049] (1) Steam explosion treatment of fish skin and fish scales:

[0050] Use the fish skin and fish scales collected in the grass carp processing process as raw materials, wash and remove impurities, steam-explode at 0.9MPa for 0.5min, release the pressure, and take them out by blasting; the raw materials after steam-explosion are obtained;

[0051] (2), water extraction:

[0052] Add 10 mass times of water to the raw material after steam explosion, mix evenly, extract at 70°C for 1 hour, and filter to obtain fish skin and scale extract;

[0053] (3), heat preservation enzymatic hydrolysis:

[0054] First adjust the pH of the extract to 7, then add the neutral protease (enzyme activity is 0.8AU / g) sold by Novozymes, the amount of the neutral protease is 0.5% of the weight of the raw material after steam explosion, 55 Enzyme hydrolysis at ℃ for 1h; ...

Embodiment 3

[0064] (1) Steam explosion treatment of fish skin and fish scales:

[0065] Use the fish skin and scales collected during the processing of herring as raw materials, wash and remove impurities, steam-explode at 1.2MPa for 0.5min, release the pressure, and take out after explosion; the raw materials after steam-explosion are obtained;

[0066] (2), water extraction:

[0067] Add 8 times the mass of water to the raw material after steam explosion, mix evenly, extract at 60°C for 2 hours, and filter to obtain the fish skin and scale extract;

[0068] (3), heat preservation enzymatic hydrolysis:

[0069] First adjust the pH of the extract to 7.5, then add trypsin (enzyme activity is 250U / mg), the amount of trypsin is 1% of the weight of the raw material after steam explosion, enzymolysis at 55°C for 2h;

[0070] (4), inactivate enzyme:

[0071] After the enzymolysis time is up, inactivate the enzyme at 90°C for 15 minutes, cool to 60°C, filter to remove the insoluble residue, a...

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Abstract

The invention discloses a preparation method of fishskin/scale collagen peptide, comprising the following steps: by taking at least one of fishskin and scale as a raw material, washing the raw material, removing impurities, and then performing steam explosion treatment; adding water into the raw material subjected to steam explosion, and performing water extraction; regulating the pH of an extracting solution to 6.0 to 8.5, then adding a protease preparation, and performing enzymolysis reaction at 40 to 65 DEG C for 0.5 to 2h; performing enzyme deactivation on an enzymolysis product, and filtering to obtain a crude enzymatic hydrolysate; performing ultrafiltration on the crude enzymatic hydrolysate by adopting an ultrafiltration membrane, wherein an obtained permeate solution is used as arefined solution; vacuum-concentrating the refined solution to the solid matter mass content of 25% to 40%, and then spray-drying, wherein the obtained fishskin/scale collagen peptide has the averagerelative molecular weight being smaller than 3kDa, and the protein content exceeding 95%. The preparation method of the fishskin/scale collagen peptide can solve the problem of utilization difficultyof the fishskin and the scale in fish processing, thus improving the added value of aquatic product processing leftovers.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a method for preparing collagen peptides by hydrolyzing fish skin and scales with commercial protease. Background technique [0002] Collagen peptide is a hydrolyzed product of collagen, which is widely used in food, medicine, chemical industry and other fields because of its low molecular weight, easy absorption, and certain biological activity. At present, the raw materials for the production of collagen products are basically the skin and bones of terrestrial animals such as pigs and cattle. Due to the scare of mad cow disease and other reasons, the use of collagen and its products is limited. Finding collagen raw materials other than pig, cowhide and bone has become a top priority, and aquatic product processing waste, especially fish skin and fish scales, is an ideal alternative raw material. [0003] As a large aquatic product processing country, my country produces a lar...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 张益奇涂丹戴志远
Owner ZHEJIANG GONGSHANG UNIVERSITY
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