Method of simultaneously extracting high purity tyrosine and levodopa from seed of stizolobium cochinchinensis

A technology of levodopa and tyrosine, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problem of not involving active ingredients - tyrosine extraction and separation, complex process, high equipment requirements problems, to achieve the effect of low cost, strong operability, and improved utilization

Active Publication Date: 2017-07-14
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the method is complex and requires high equipment, and the content of levodopa in the extract is no more than 15%.
[0014] Among the above-mentioned methods, only the

Method used

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  • Method of simultaneously extracting high purity tyrosine and levodopa from seed of stizolobium cochinchinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Grinding and enzymatic hydrolysis of raw materials: Grind 100kg of cat bean raw materials with a low-temperature pulverizer until the particle size is 80 mesh, then add 100kg of water, 1.5kg of protease preparation and 0.15kg of vitamin E, mix well, and enzymatically hydrolyze at 20°C 10h, get 201kg enzymatic hydrolyzate;

[0051] (2) Extraction and nanofiltration: Add 1100 kg of methanol aqueous solution with a volume concentration of 20% to the 201 kg of enzymatic hydrolyzate obtained in step (1), stir and extract at room temperature for 4 hours, filter, and use a nanofiltration membrane with a molecular weight cut-off of 1.0 kDa to Filter to get 1000kg permeate;

[0052] (3) Concentration and crystallization: Concentrate 1000kg of the permeate obtained in step (2) with a falling film concentrator until the total solids mass concentration is 15%, cool to room temperature, and adjust the pH value with 5% hydrochloric acid aqueous solution to 5.3, stirred and cryst...

Embodiment 2

[0056] (1) Grinding and enzymatic hydrolysis of raw materials: Grind 200kg of cat bean raw materials with a low-temperature pulverizer until the particle size is 100 mesh, then add 400kg of water, 8kg of protease preparation and 0.8kg of β-carotene, mix well, and perform enzymatic hydrolysis at 30°C 7h, get 605kg enzymolyzate;

[0057] (2) Extraction and nanofiltration: Add 9075 kg of ethanol aqueous solution with a volume concentration of 30% to the 605 kg of enzymatic hydrolyzate obtained in step (1), stir and extract at room temperature for 6 hours, filter, and use a nanofiltration membrane with a molecular weight cut-off of 0.5 kDa to Filter to get 9000kg permeate;

[0058] (3) Concentration and crystallization: Concentrate 9000kg of the permeate obtained in step (2) with a falling film concentrator to a total solid mass concentration of 20%, cool to room temperature, and adjust the pH value with an aqueous solution of sulfuric acid with a mass concentration of 8%. to 5.7...

Embodiment 3

[0062] (1) Grinding and enzymatic hydrolysis of raw materials: Grind 150kg of cat bean raw materials with a low-temperature pulverizer until the particle size is 80 mesh, then add 150kg of water, 3kg of protease preparation and 0.15kg of tea polyphenols, mix well, and enzymatically hydrolyze at 20°C 12h, get 302kg enzymatic hydrolyzate;

[0063] (2) Extraction and nanofiltration: Add 3020 kg of methanol aqueous solution with a volume concentration of 50% to the 302 kg of enzymatic hydrolyzate obtained in step (1), stir and extract at room temperature for 8 hours, filter, and use a nanofiltration membrane with a molecular weight cut-off of 2.0 kDa to Filter to get 2800kg permeate;

[0064] (3) Concentration and crystallization: Concentrate 2800kg of the permeate obtained in step (2) with a falling film concentrator to a total solid mass concentration of 25%, cool to room temperature, and adjust the pH value with 10% acetic acid aqueous solution to 5.7, stirred and crystallized...

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Abstract

The invention relates to a method of simultaneously extracting high purity tyrosine and levodopa from the seed of stizolobium cochinchinensis. The method comprises the following steps: (1) crushing a raw material, seed of stizolobium cochinchinensis, adding water, a protease preparation and an antioxidant for enzymolysis to obtain a hydrolysate; (2) adding a low degree alcohol aqueous solution to extract, filter and nano-filter to obtain a permeate liquid; (3) concentrating and cooling the permeate liquid to room temperature, adjusting the pH value, performing devitrification, filtering and drying to obtain a tyrosine coarse product; adjusting the pH value of the filtrate, and performing devitrification, filtering and drying to obtain a levodopa coarse product; and (4) adding an acidic alcohol aqueous solution into the tyrosine or levodopa coarse product separately to filter and adjust the pH value, performing freezing and devitrification, filtering and drying to obtain high purity tyrosine and levodopa. The product obtained by the method is white crystal powder; the purity of tyrosine is greater than or equal to 97.5%, the yield is greater than or equal to 99.8%, the purity of levodopa is greater than or equal to 99.8%, and the yield is greater than or equal to 91%. The method provided by the invention is high in operability, environment-friendly and pollution-free, low in cost and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting two amino acids from cat beans, in particular to a method for simultaneously extracting high-purity tyrosine and levodopa from cat beans. Background technique [0002] Cat beans, also known as Prickly Li beans, are the seeds of ivy vine. Ivy vine is mainly distributed in subtropical and temperate regions, growing in bushes, valleys, and riversides. In China, it is mainly distributed in Sichuan, Guizhou, Yunnan, southern Shaanxi, Hubei, Zhejiang, Jiangxi, Hunan, Fujian, Guangdong, Guangxi. Cat beans have the characteristics of rich and balanced nutritional value, especially rich in protein, and its nutritional components are close to those of soybeans and broad beans. In addition, cat beans are rich in levodopa, which is the most important raw material for extracting natural levodopa. Among them, the content of levodopa can reach up to 10%. In recent years, studies have found that there are many import...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C227/42C07C229/36
CPCC07C227/40C07C227/42C07C229/36
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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