Functional peptide protein powder, and preparation method and applications thereof

A technology of functional peptide and protein powder, which is applied in the field of animal-derived protein feed preparation, can solve problems such as easy moisture absorption, large composition changes, and affecting feeding effects, so as to increase the content of amino nitrogen, increase the content of protein, and The effect of improving the hydrolysis efficiency

Active Publication Date: 2014-12-03
北京澳龙港生物技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 2) The product is poor in palatability, has a bloody smell, is dark brown, and is sticky, which greatly limits the amount of product added
The composition of blood meal products changes greatly, which affects the feeding effect. This may be related to the microorganisms in the blood, especially the pathogenic bacteria. The microorganisms reproduce in large numbers, which reduces the pH and affects the nutritional value of the product.
However, blood products are prone to moisture absorption, caking, maggots, mold, and corruption, so blood products are not suitable for long-term storage
[0009] 4) The traditional processing method does not sufficiently excavate the molecular resources of hemoglobin, and the functionality of the product has not been fully utilized
In addition to the high protein content, the blood of livestock and poultry also contains carbohydrates, fats, minerals, vitamins, enzymes and some active peptides. and other functions, while the existing production and processing technologies pay more attention to the preparation of traditional nutritional products, while ignoring the functional characteristics of livestock and poultry blood
[0010] 5) In terms of processing technology, the traditional processing technology of livestock and poultry blood and its products have inherent defects
In the process of common processing, the cells in the blood harden, poor in palatability, which is not conducive to digestion and absorption; the content of crude protein in the blood meal processed by fermentation is low and unstable, the fermentation time is long, and the amount of carrier is large; although the enzymatic hydrolysis method is relatively mild, protease bitter peptide

Method used

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  • Functional peptide protein powder, and preparation method and applications thereof
  • Functional peptide protein powder, and preparation method and applications thereof
  • Functional peptide protein powder, and preparation method and applications thereof

Examples

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preparation example Construction

[0099] ②Preparation of serine standard solution: 50mg of serine was dissolved in 500ml of deionized water (0.9516meqv / L).

[0100] ③Preparation of sample solution: Xg sample is dissolved in 100ml deionized water, X is 0.1-1.0g sample containing 8%-80% protein.

[0101] ④Standard measurement: Add 400μL of serine standard solution into a stoppered test tube containing 3mL of OPA reagent, shake well, react accurately at room temperature for 2min, and then measure its absorbance at 340nm with a UV spectrophotometer.

[0102] ⑤ Blank measurement: Add 400 μL deionized water to a stoppered test tube containing 3 mL of OPA reagent, shake well, react accurately at room temperature for 2 min, and then measure its absorbance at 340 nm.

[0103] ⑥Sample detection: Add 3 mL of OPA reagent to 0.4 μL of the sample to be tested and mix well, let it stand at room temperature for 2 minutes, and then measure its absorbance at 340 nm.

[0104] ⑦Calculation:

[0105]

[0106]

[0107] In t...

Embodiment 1

[0119] Example 1 Preparation of functional peptide protein powder E-1

[0120] (1) Preparation of blood cells: Collect fresh duck blood from the slaughterhouse in a safe and hygienic way, and then add 0.6% sodium citrate to the total blood weight to prevent blood coagulation. Transport the duck blood with sodium citrate to the processing plant in the shortest possible time, and centrifuge it with a high-speed centrifuge at a speed of 14,000r / min for 10 minutes. At this time, the blood is divided into upper and lower layers, and the upper layer is light yellow plasma. , stored for other purposes; the lower layer is dark red viscous blood cells.

[0121] (2) Cell wall breaking: add water and hydrochloric acid solution with a concentration of 0.25mol / L to the blood cells obtained by centrifugation to prepare blood cell dilution, the mass ratio of hydrochloric acid solution, blood cells, and water is 1:4:5, and then make the substrate The concentration was maintained at 15g / 100mL...

Embodiment 2

[0128] Example 2 Preparation of functional peptide protein powder E-2

[0129] (1) Preparation of blood cells: Collect fresh duck blood from the slaughterhouse in a safe and hygienic way, and then add 0.8% sodium citrate to the total blood weight to prevent blood coagulation. Transport the duck blood with sodium citrate to the processing plant in the shortest possible time, and centrifuge it with a high-speed centrifuge at a speed of 16,000r / min for 8 minutes. At this time, the blood is divided into upper and lower layers, and the upper layer is light yellow plasma. , stored for other purposes; the lower layer is dark red viscous blood cells.

[0130] (2) Cell wall breaking: add water and hydrochloric acid solution with a concentration of 0.05mol / L to the blood cells obtained by centrifugation to prepare blood cell dilution, the mass ratio of hydrochloric acid solution, blood cells, and water is 1:3:6, and then make the substrate The concentration was maintained at 11.5g / 100m...

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Abstract

The present invention discloses a method for preparing functional peptide protein powder using blood cells of livestock and poultry as raw materials. The method is as follows: continuously centrifuging fresh blood of livestock and poultry to separate blood plasma and blood cells; processing the blood cells by physical and chemical methods to break cell membranes and denature intracellular protein; after the membrane breaking and denaturing process, adding acid (or base) and a protease preparation into the blood cell solution to enzymatically hydrolyze the blood cell protein into small peptides and amino acids containing functional ingredients; then centrifuging the products of enzymolysis and adding acid and a decolorizer to performing decoloration; and finally performing spray drying on the decolorized blood cell solution. The present invention also provides functional peptide protein powder prepared by the method. The functional peptide protein powder has a high protein content, a high essential amino acid content, a high bioactive peptide content, a high digestion and absorption rate, and a strong antioxidant capacity, can improve the disease resistance of livestock and poultry, protein deposition and production performance, can be widely used for livestock, poultry and aquatic animals, and can also be used as a substitute for fish meal and soybean meal.

Description

technical field [0001] The invention belongs to the technical field of animal-derived protein feed preparation, and in particular relates to a functional peptide protein powder and its preparation method and application. Background technique [0002] With the rapid development of the animal husbandry industry, the global livestock and poultry production continues to rise, which drives the growth of feed production. In 2012, the global feed production reached 959 million tons, while my country's feed production ranks first in the world. With the increase of feed production, the demand for feed raw materials has increased, especially protein raw materials with important nutritional and physiological functions, which have become the largest raw material supply bottleneck affecting the development of animal husbandry in my country. Therefore, how to develop and utilize existing feed protein resources, improve the effective utilization rate and quality of protein, increase revenu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/06A23J3/34A23K1/04A23K1/16A23K1/18A23K10/14A23K20/147A23K50/30A23K50/75A23K50/80
CPCY02A40/70Y02A40/818
Inventor 张日俊郑召君黄燕
Owner 北京澳龙港生物技术研究中心
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