Porphyridium concentrated harvesting method and porphyridium culturing and concentrated harvesting coupling method

Abstract

The invention discloses a porphyridium concentrated harvesting method and a porphyridium culturing and concentrated harvesting coupling method. The porphyridium concentrated harvesting method comprises the following steps of culturing porphyridium, when the density of porphyridium cells inside culture mediums reach an appropriate harvesting density and the content of exopolysaccharides reaches above 0.02 g/L, inletting gas into the culture medium to perform foam separation concentration, collecting surface bubbles of the culture medium, and then cracking the bubbles to obtain concentrated porphyridium extract. The porphyridium concentrated harvesting method saves surfactant and flocculant, but completely depends on the exopolysaccharides produced by porphyridium to achieve the effects of foam concentration; when the porphyridium is cultured to a stable growth period and secretes a certain quantity of the exopolysaccharides, by changing the structure of a gas distributor to produce tinybubbles, high-concentration harvesting of the porphyridium can be achieved. The porphyridium concentrated harvesting method can achieve concentrating effects by 60 times, greatly save time of centrifugation and reduce the harvesting cost.

Description

Technical field: [0001] The invention belongs to the technical field of marine microbes, and in particular relates to a method for concentrating and harvesting Porphyridum algae and a method for coupling the cultivation of Porphyrococcus and concentrating and harvesting. Background technique: [0002] Porphyridium (Porphyridium) is a marine unicellular algae belonging to Rhodophyta, Rhodophyceae, Rhodophyllia, Porphyrides, Porphyridaceae, and Porphyridium. Environmental adaptability and rapid reproduction ability can synthesize a variety of biologically active ingredients, showing broad application prospects and huge market space in the fields of medicine, cosmetics, fine chemicals, food, and health care products. [0003] Porphyridium, like other photoautotrophic organisms, needs a small amount of inorganic salts such as nitrogen, phosphorus, carbon, and iron, and at the same time gives a certain amount of light to achieve artificial cultivation. Porphyridium has strong en...

AI Technical Summary

Problems solved by technology

At the beginning of the 20th century, foam flotation was widely used in the mining and metallurgy industry, and was later used to remove surface active substances (such as surfactants, protein enzymes, etc.) and detergents in wastewater; in the field of biochemical products, it can also be separated by foam technology for virus isolation and protein enzyme refining, but there is no report on the relevant patents of this method for microalgae harvesting

[0011] (1) At present, the only feasible Porphyridium algae harvesting technology is high-speed centrifugation. High-speed centrifugation has the disadvantages of large equipment investment, high energy consumption, low efficiency, and potential safety hazards in high-speed operation;

[0012] (2) The prior art cannot realize the simultaneous cultivation and harvesting of Porphyridium algae;

[0013] (3) The open cultivation technology of the existing Porphyridium can not realize the high-efficiency supplement of carbon dioxide;

[0014] (4) The energy consumption of centrifugal harvesting is huge;

[0016] (6) There are potential safety hazards in the process of air flotation harvesting of Porphyridium algae with flocculants

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