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Antioxidant active peptide fragment derived from sea cucumbers and extraction method

A technology of antioxidant active peptides and antioxidant activities, which is applied in the field of preparation and identification of antioxidant active peptides, can solve the problems of unfavorable absorption, etc., and achieve the effect of strong antioxidant activity and clear sequence

Active Publication Date: 2018-06-08
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the special evolutionary status of sea cucumber, unique reproductive life history, and biological phenomena such as aestivation, viscera removal and regeneration, and autolysis, it has important scientific research value. At the same time, there are relatively few studies on its active peptides. At present, the sea cucumber mixed polypeptide mainly prepared by enzymatically hydrolyzing sea cucumber protein with protease has a molecular weight of more than 5000 Da, which is not conducive to human body absorption, and it is also a mixture of peptides with various functions.

Method used

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  • Antioxidant active peptide fragment derived from sea cucumbers and extraction method
  • Antioxidant active peptide fragment derived from sea cucumbers and extraction method
  • Antioxidant active peptide fragment derived from sea cucumbers and extraction method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A peptide with antioxidant activity derived from sea cucumber, the amino acid sequence of the peptide is NH 2 -Gly-Pro-Ala-Gly-Pro-Cys. The peptide has a good scavenging effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals.

Embodiment 2

[0036] A method for extracting antioxidant active peptides derived from sea cucumbers described in Example 1, specifically comprising the following steps:

[0037] S1, raw material pretreatment: 1000 kg of imitation sea cucumber body wall is pulverized with a meat grinder for 15 minutes, and then entered into a colloid mill for grinding to obtain a slurry;

[0038] S2, import the slurry into 5m 3 Enzymolysis tank, the 5m 3 The enzymolysis tank is a sandwich type heating reaction kettle, add pure water 3 times the mass of oyster slurry, adjust the system temperature to 50°C, adjust the pH to 8.6 with 2.5mol / L NaOH solution, add 0.12% of the total mass of the imitation sea cucumber slurry The compound protease of compound protease; the mass ratio of compound protease is: neutral protease: papain: flavor protease=3:4:3; Stir enzymolysis 4h, heat up 80 ℃ and kill enzyme 20min, adopt tubular centrifuge 16000rpm centrifugation to carry out solid-liquid Separation, collection of ce...

Embodiment 3

[0047] The extraction method of the antioxidant active peptides derived from sea cucumbers described in this example is the same as that in Example 2, and the different technical parameters are:

[0048]1) In step S1, the body wall of the imitation sea cucumber was crushed with a meat grinder for 10 minutes;

[0049] 2) In step S2, add pure water twice the mass of the oyster slurry, adjust the system temperature to 40°C, adjust the pH to 8.0 with 2.5mol / L NaOH solution, and add a compound protease of 0.1% of the total mass of the japonicus japonicus slurry; compound The mass ratio of protease is: neutral protease: papain: flavor protease = 2:3:4; stir for 3 hours, heat up to 82°C to extinguish the enzyme for 10 minutes, and use a tubular centrifuge at 10,000rpm to separate the solid from the liquid;

[0050] 3) In step S3, the obtained clear liquid is separated by a nanofiltration membrane separation device with a cutting molecular weight of 2000Da to obtain the permeate;

[...

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Abstract

The invention belongs to the fields of preparation and identification of antioxidant active peptides, and particularly relates to an antioxidant active peptide fragment derived from sea cucumbers andan extraction method. The amino acid sequence of the peptide fragment is NH2-Gly-Pro-Ala-Gly-Pro-Cys, and the peptide fragment is obtained by enzymolysis by using a compound protease preparation, andhas a good scavenging effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals. Sea cucumber oligopeptides with the molecular weight of 400-800 account for 57%-68% of the total sea cucumber oligopeptides prepared by the method provided by the invention. The sea cucumber oligopeptides prepared by the method have no bitterness or fishy smell, and can be directly eatenas a protein nutritional supplement. The peptide fragment obtained by the method is derived from apostichopus japonicus, has strong antioxidant activity and a clear sequence, and can be produced massively by using prokaryotic expression or polypeptide synthesis method.

Description

technical field [0001] The invention belongs to the field of preparation and identification of antioxidant active peptides, in particular to an antioxidant active peptide segment derived from sea cucumbers and an extraction method. Background technique [0002] Because there are many extreme environments in the ocean, such as high pressure (deep sea), low temperature (polar regions, deep sea), high temperature (undersea crater) and high salinity, etc. In order to adapt to these extreme marine habitats, marine biological proteins are very different from terrestrial biological proteins regardless of amino acid composition or sequence. Among the various marine protein amino acid sequences, there are many potentially biologically active amino acid sequences, which are hydrolyzed with specific proteases to release active peptides. The biological significance of these active peptides is mainly reflected in two aspects: their absorption mechanism is better than that of amino acids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/14
CPCC12P21/06C07K7/06
Inventor 包卫洋王祖哲杨大苹马普
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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