Two-step fermentation method for efficient production of fermented soybean meal

A technology for fermenting soybean meal and soybean meal, which is applied in animal feed, animal feed, application, etc., can solve problems such as uncomfortable micro-aerobic environment, and achieve the effects of uniform fermentation degree, balanced animal intestinal microbial environment, and simple fermentation method.

Active Publication Date: 2014-09-03
JIANGNAN UNIV
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Problems solved by technology

[0004] Bacillus subtilis, as a strain with high protease production, is often used to ferment soybean meal, but since most protease-producing Bacillus subtilis are aerobic bacteria, they are not suitable for the micro-aerobic environment of stacking fermentation, so only some extra can be added in stacking fermentation production Protease preparations to enhance protein degradation

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  • Two-step fermentation method for efficient production of fermented soybean meal
  • Two-step fermentation method for efficient production of fermented soybean meal

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Embodiment 1

[0018] Two-step fermentation is carried out with Bacillus subtilis and plant lactic acid bacteria as the main fermentation strains. The specific steps are as follows:

[0019] (1) Strain activation: Bacillus subtilis was activated with beef extract peptone medium for 2 days, then two rings were placed in 100mL culture solution at 37°C, 200r / min shaking table for 20 hours; lactic acid bacteria were activated with MRS medium for 2 days, and then two rings were placed The rings were cultured statically at 30°C for 48 hours in 100 mL of culture medium.

[0020] (2) Soybean meal crushing: use a pulverizer to crush the soybean meal, and pass through a 60-mesh sieve to obtain soybean meal less than 60 mesh for subsequent fermentation.

[0021] (3) Shallow plate fermentation: take two wide-mouth can bottles, add 50g of crushed soybean meal and 50mL of water to each bottle, mix evenly, wrap it with eight layers of gauze and newspaper, and sterilize at 121°C for 20 minutes. After cooli...

Embodiment 2

[0026] Two-step fermentation is carried out with Bacillus subtilis, Neurospora and plant lactic acid bacteria as the main fermentation strains, and the specific steps are as follows:

[0027] (1) Strain activation: Bacillus subtilis was activated with beef extract peptone medium for 1-2 days, and then two rings were placed in 100mL culture solution at 37°C, 200r / min shaker for 20h; Neurospora was activated with PDA medium, Take two rings of activated Neurospora spores and disperse them in 100mL of sterilized sterile water, which is the bacterial suspension of Neurobacillus; lactic acid bacteria are activated with MRS medium for 2 days, and then put two rings in 100mL culture solution at 30°C Static culture for 48h.

[0028] (2) Shallow dish fermentation: Take four canned jars, add 50g of crushed soybean meal and 50mL of water to each bottle, mix evenly, wrap with eight layers of gauze and newspaper, and sterilize at 121°C for 20min. After cooling down, add 2 mL of Bacillus su...

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Abstract

The present invention discloses a two-step fermentation method for efficient production of fermented soybean meal, and belongs to the technical field of solid-state microorganic fermentation. The present invention adopts soybean meal as main raw material and uses an aerobic bacteria-bacillus subtilis with high protease production capacity as a major strain of shallow dish fermentation in the first step. The fermented soybean meal with high protease activity is obtained through an aerobic fermentation for 1-2 days. The shallow dish fermented soybean meal is served as a protease inhibitor-like agent to mix with lactobacillus and other unsterilized soybean meal to conduct the second step-heap fermentation. The present invention not only can meet the requirement of mix fermentation of aerobic and anaerobic bacteria, but also solves the problem that the aerobic bacteria is difficult to apply to solid heap accumulation. The shallow dish fermented soybean meal has a protease activity of higher than 700U / g, and the soybean meal after heap fermentation has a crude protein content of higher than 50% and a small molecule peptide content higher than 10%. The mixed fermentation can improve the palatability of soybean meal for animals, promote digestion and absorption of animals, balance animal intestinal microbial environment and improve animal immunity.

Description

technical field [0001] The invention relates to a method for efficiently producing fermented soybean meal by two-step fermentation, which belongs to the technical field of solid-state microbial fermentation. Background technique [0002] Soybean meal is a by-product of soybean oil extraction. It has high protein content and rich amino acid composition, and is a widely used vegetable protein resource. Fermented soybean meal is the combination of microbial technology and solid-state fermentation technology, using soybean meal as the main raw material, inoculating microorganisms, eliminating the anti-nutritional factors in soybean meal to the greatest extent through microbial fermentation, effectively degrading soybean protein into a high-quality small peptide protein source, and Probiotics, oligopeptides, glutamic acid, lactic acid, vitamins, UGF (unknown growth factor) and other active substances can be produced. For animals, it has the effects of improving palatability, imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14A23K10/12A23K10/37
CPCY02P60/87
Inventor 杨瑞金郑婷婷金妙仁徐张贤赵伟
Owner JIANGNAN UNIV
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