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Preparation method of food-grade low-salt-content sea fish oligopeptide powder

A marine fish oligopeptide, food-grade technology, applied in the field of preparation of marine fish active peptides, can solve the problems of high cost, general desalination effect, high energy consumption, etc., to improve quality, wide enzymatic hydrolysis sites, and reduce pressure Effect

Active Publication Date: 2015-11-18
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Usually, the salt content of marine fish meal is 2-5%. Adjusting the pH value during the enzymatic hydrolysis process will bring in some salt. 25%, while the effect of desalination purely by membrane separation is mediocre, and the energy consumption is high and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Food-grade marine low-fat fishmeal is accurately weighed by a measuring scale, mixed with pure water in a pre-desalination mixing tank according to the mass ratio of material to water at 1:5, set the temperature at 25°C, stirred at a speed of 60r / min, washed with water for 10min, and passed through 100 mesh The pre-desalted fish meal is obtained by pressure filtration with a diaphragm filter press, and the desalination rate is 86.2%. The pre-desalted fish meal is transported to the enzymatic hydrolysis pre-mixing tank through a screw conveyor, and pure water is injected according to the mass ratio of material to water at 1:8, and stirred at 60r / min After mixing, pump it into the colloid mill through the screw pump, use the colloid mill to grind the pre-desalted fish meal particles to 60 mesh, and then transport it to the enzymolysis tank through the screw pump to prepare for enzymolysis; control the temperature at 50°C and pH 9.0, according to the base Add Novozymes alka...

Embodiment 2

[0023] Food-grade marine low-fat fish meal is accurately weighed by a measuring scale, mixed with pure water in a pre-desalination mixing tank according to the mass ratio of material to water 1:6, set the temperature at 30°C, and the stirring speed is 60r / min, washed with water for 20min, and then passed through 100 mesh Pre-desalted fish meal is obtained by pressure filtration with a diaphragm filter press, and the desalination rate is 89.8%; the pre-desalted fish meal is transported to the enzymatic hydrolysis pre-mixing tank through a screw conveyor, and pure water is injected according to the mass ratio of material to water at 1:10, and stirred at 60r / min After mixing, it is pumped into the colloid mill through the screw pump, and the pre-desalted fish meal particles are ground and pulverized to 80 mesh by the colloid mill, and then transported to the enzymolysis tank through the screw pump to prepare for enzymolysis; Add 0.8L of Novozymes neutral protease Neutrase to 0.8% ...

Embodiment 3

[0026]Food-grade marine low-fat fishmeal is accurately weighed by a measuring scale, mixed with pure water in a pre-desalination mixing tank according to the mass ratio of material to water 1:10, set the temperature at 50°C, stirring at a speed of 60r / min, washed with water for 30min, and then passed through 100 mesh The pre-desalted fish meal is obtained by pressure filtration with a diaphragm filter press, and the desalination rate is 94.8%; the pre-desalted fish meal is transported to the enzymatic hydrolysis pre-mixing tank through a screw conveyor, and pure water is injected according to the mass ratio of material and water at 1:6, and stirred at 60r / min After mixing, it is pumped into the colloid mill through the screw pump, and the pre-desalted fish meal particles are ground and pulverized to 100 mesh by the colloid mill, and then transported to the enzymolysis tank through the screw pump to prepare for enzymolysis; the temperature is controlled at 55°C and pH8.5, accordi...

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Abstract

The invention provides a preparation method of food-grade low-salt-content sea fish oligopeptide powder, which uses food-grade sea low-fat fish powder as the raw material and comprises the following steps: pre-desalting by water washing, milling and pulverizing by a colloid mill, carrying out enzymolysis, carrying out membrane separation, concentrating and carrying out spray drying. The raw material fish powder is pre-desalted by water washing to remove most salts in the raw material fish powder, thereby relieving the pressure for subsequent nanofiltration membrane desalting. The food-grade industrialized proteinase preparation is used for enzymolysis, and a 1000-10000Da ultrafiltration membrane is utilized to separate the enzymolysis solution, thereby removing pigments and peptides with higher molecular weight; and a 150-500Da nanofiltration membrane is utilized to remove free amino acids and residual salts in the enzymolysis solution. The prepared food-grade low-salt-content sea fish oligopeptide powder has the advantages high oligopeptide content and low salt content, can be widely used in food industry, and is suitable for large-scale industrial production.

Description

technical field [0001] This patent belongs to the field of preparation of marine fish active peptides, and specifically relates to the preparation of food-grade low-salt marine fish oligopeptides with molecular weight less than or equal to 1000Da. Background technique [0002] Oligopeptide refers to a class of functional biologically active peptides consisting of 2 to 10 amino acids and a molecular weight of 180 to 1000 Da. Studies have shown that oligopeptides have strong biological activities, including: regulating hormone levels in the body; improving mineral and trace element absorption; resisting bacteria and viruses; improving immunity; inhibiting and destroying tumor cells; anti-oxidation and scavenging free radicals, etc. [0003] my country has a vast sea area and abundant marine fish resources. With the rapid development of the marine fishing industry, the output of low-value fish caught is on the rise, accounting for about 60% of the marine fishing output. Due to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 王立梅齐斌
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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