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Alkane degrading bacteria and application thereof

A technology of alkane degrading bacteria and Tsukamura bacterium is applied in the field of alkane degrading bacteria to achieve the effect of promoting biodegradation, increasing contact area and improving solubility

Inactive Publication Date: 2012-10-03
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the existing problems of petroleum pollutants, and aims to provide an alkane-degrading bacterium and its application, which can efficiently degrade alkane components in petroleum pollutants

Method used

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  • Alkane degrading bacteria and application thereof
  • Alkane degrading bacteria and application thereof
  • Alkane degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Tsukamura ( Tsukamurella sp . ) Separation and identification of C20 and its degradation performance on alkanes

[0015] The source of the bacteria was collected from the polluted soil in the sewage treatment station of Wuhan Branch of China Petroleum & Chemical Corporation, 2 to 4 cm from the surface of the oil-sludge mixture, which was dark brown.

[0016] Add 2 g of the collected soil sample to 100 mL of LB medium containing 200 mg / L n-hexadecane (peptone 1wt%, sodium chloride 1wt%, yeast extract 0.5 wt%), and add 15-20 The glass beads of bacteria were activated and cultured at 30°C and 150 r / min for 24 h. After standing still for 10 min, take 2 mL of culture medium and transfer to 100 mL of n-hexadecane inorganic salt culture concentration, acclimate to culture under the same conditions for 1-2 days, and transfer 4 times with gradient, in which the concentration of n-hexadecane is 200, 500 , 1000, 2000 mg / L. Gradually dilute the culture solution con...

Embodiment 2

[0020] Example 2: pH on Tsukamura ( Tsukamurella sp . ) Effect of C20 degradation performance

[0021] Inoculate the inorganic salt medium with 2vol% Tsukamurella ( Tsukamurella sp.) C20 bacterial suspension, adjust the inorganic salt medium to a different pH value (5.0 ~ 8.0), under the condition of the initial n-hexadecane concentration of 1000mg / L, cultivate for 36 hours, and measure the effect of pH value on the degradation of C20 The effect of n-hexadecane, the results are shown in Table 1. It is shown from Table 1 that in the range of pH 5.0-7.0, with the increase of pH in the medium, the ability of strain C20 to degrade alkanes increases, and when the pH is 7, the degradation ability is the strongest, and the degradation rate reaches 79.4%; when the pH is 8.0 When the pH was 6.0, the degradation rate dropped to 54.2%, but it reached 65.1% when the pH was 6.0, indicating that the strain C20 had a strong ability to degrade alkanes in a neutral or slightly acidic en...

Embodiment 3

[0025] Example 3: Tsukamura ( Tsukamurella sp . ) C20 degradation of diesel

[0026] 2vol% of Tsukamura ( Tsukamurella sp .) C20 was inoculated in the inorganic salt medium supplemented with 800mg / L diesel oil, cultured on a shaker at 37°C and 150r / min, cultured for 8, 12, 18 and 23 hours respectively, and samples were taken to analyze the strain’s resistance to diesel oil by gas chromatography. Degradation rate, while taking the sample without adding bacteria as the control. From Table 2 and figure 2 It can be seen that although in the initial 12 h, the volatilization loss of diesel components reached 14.2%, but adding the strain Tsukamurella sp. The degradation rate of C20 has reached 58.1%, indicating that the bacteria can degrade most of the alkanes in diesel. from figure 2 It can be seen that the bacteria are sensitive to alkane components (C 12 ~C 24 ) have a good degradation effect.

[0027] Table 2 Degradation effect of strain C20 on diesel

[0028] ...

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Abstract

The invention discloses an alkane degrading bacterium and an application thereof. The alkane degrading bacterium C20 provided by the invention belongs to Tsukamurellasp. The alkane degrading bacterium is preserved in China Center for Type Culture Collection on 23rd, March, 2012; the preservation number is CCTCC NO: M2012089; the GenBank accession number of the strain 16SrDNA is JQ806393. The alkane degrading bacterium is a gram-positive bacterium; the bacterial colony is chrysanthemum yellow, has regular edge, is round, has drying surface and raised centre; the thallus is in short-rod shape, is arranged in a chain shape and has no capsule under observation of a microscope. The degradation rate of the alkane degrading bacterium for 1000mg / L of n-hexadecane in 48h is more than 98%; medium-long-chain alkane (C12-C24) in the diesel can effectively degraded, and the alkane degrading bacterium metabolizes to produce a bio-emulsifier for accelerating oil to dissolve. The alkane degrading bacterium can be applied in biological strengthening treatment and repairing of refinery waste water, petroleum-polluted water and soil and has good prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant bioaugmentation, and in particular relates to an alkane-degrading bacterium and its application. Background technique [0002] With the development of the petroleum industry, a large amount of petroleum enters the atmosphere, water and soil through various channels during the process of petroleum exploration, transportation and refining, which has caused serious environmental pollution. According to the U.S. Environmental Protection Agency (EPA), there are about 3 to 5 million underground chemical and oil storage warehouses in the United States, and an average of about 1% of the warehouses will leak every year, seriously affecting the quality of surface water and groundwater. Great harm to human health. There are about 110,000 gas stations in our country. The gas stations are so densely distributed in the streets and alleys of the city. Once there is a problem, it will cause great...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
Inventor 颜家保姚嫚王巧凤魏鑫游海许龙龙
Owner WUHAN UNIV OF SCI & TECH
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