39results about How to "Promote bioremediation" patented technology

A process for the remediation and conditioning of soil containing a contaminant. The process comprises (a) mixing the soil with solid material comprising a source of microbial nourishment, (b) inoculating the mixed soil with a diverse microbe population capable of digesting the contaminant, and (c) maintaining the moisture content of the inoculated soil at a level sufficient to support microbial growth. The process can include the additional steps of applying a suspension fertilizer to the mixed soil and/or applying an oxygen-releasing compound to the mixed soil. Step (b) can be repeated to sufficiently reduce the level of contamination.

The invention discloses an aquaculture water quality balancing purification agent, which is prepared from the following raw materials by weight: 10-25 parts of tourmaline, 20-40 parts of montmorillonite, 5-10 parts of photosynthetic bacteria, 8-24 parts of actinomycetes, 40-60 parts of zeolite, 10-15 parts of bacillus subtilis, 10-15 parts of Streptococcus faecalis, 10-25 parts of Soybean molasses, 5-10 parts of sodium dihydrogen phosphate, 3-6 parts of tryptophan, 8-16 parts of sodium humate, 10-15 parts of sodium thiosulfate, and 10-20 parts of nitrobacteria. The aquaculture water quality balancing purification agent provided by the invention can reduce harmful substances in water, balance water nutrition, control outbreak of cyanobacteria, activate growth of beneficial algae, increase the dissolved oxygen of water, and improve the content of beneficial microorganisms.

The invention discloses a microbial strain for degrading long-chain alkanes and application of the microbial strain. The microbial strain is acinetobacter junii numbered as C12, and is collected in China General Microbiological Culture Collection Center on May 30, 2019 with the collection number of CGMCC NO. 17873. A biological emulsification effect can be achieved during culture of the microbialstrain, alkane solubilization and emulsification can be promoted, and the alkanes are easily degraded and used as carbon sources or energy by microorganisms. The strain is easy to culture, has high degradation efficiency, and has a potential in wide application to green and efficient remediation of petroleum hydrocarbon contaminated soil and water.

The invention belongs to the field of microorganism and relates to rhizobium capable of degrading polychlorinated biphenyl and an application thereof. Sinorhizobium melilon SL1 is preserved in China General Microbiological Culture Collection Center, CGMCC on December, 10th, 2012. The collection number is CGMCC No.6962. The strain can grow by the utilization of polychlorinated biphenyl as the sole carbon source and energy, and has good capability of degrading both 2,4,4'-TCB and 3,3',4,4'-PCB under the condition of a shake flask in a laboratory. Degradation conditions are optimized, and the polychlorinated biphenyl degrading effect of the strain is further raised. The strain provides foundations for biodegradation of polychlorinated biphenyl and water body pollution restoration.

A method for facilitating growth of microbial communities for in-situ bodies of flowing water for the sake of bio-remediating contaminated water and/or producing useful biomass.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas capable of oxidizing antimony and arsenic and application of the pseudomonas. A pseudomonas strain is separated and screened from antimony-polluted soil in a stannary in Lengshuijiang City, Hunan Province and has efficient antimony and/or efficient arsenic oxidation performance; bacteria can still survive in a solution and soil with high antimony content and/or arsenic content, has relatively good antimony tolerance and arsenic tolerance and has excellent antimony arsenic oxidability and/orarsenic oxidability after being tested, so that an important foundation is laid for development of a technique for removing antimony and/or arsenic through adsorption of the microorganisms. Meanwhile,the strain combined with Fe<3+> has a good improvement effect, the oxidizing efficiency of the strain can be improved, and the strain has good development and application potentialities.

The invention relates to the technical field of management of oil polluted environments, in particular to an oleophylic fertilizer for bioremediation and a method for preparing the oleophylic fertilizer. The oleophylic fertilizer is composed of lecithin, oleic acid, nitrogen-phosphorus solution and alcohols. The preparation method includes: mixing the lecithin with the alcohols proportionally, heating, ultrasonically dissolving, adding the nitrogen-phosphorus solution and oleic acid of a certain quantity, and performing vortex oscillating to form water-in-oil microemulsion, so that the product is obtained and sealed for storage. After the oleophylic fertilizer contacts with oil pollutants, the oil pollutants can form fine oil drops to be aggregated, the oleophylic fertilizer is attached onto the oil drops to provide nitrogen and phosphorus source needed by oil degrading microorganisms, and bioremediation effect can be remarkably improved.

A composition containing cAMP, cGMP, forskolin, adenylate cyclase or guanylate cyclase and microorganisms is provided to facilitate bioremediation, detoxication and to enhance plant growth in media contaminated with petroleum hydrocarbons. Methods to make the composition and apply it to the contaminated media in order to facilitate bioremediation and detoxication of such contaminants are also provided.

A recombinant bacterium capable to completely degrade or mineralize pollutants such as polychlorobiphenyls (PCBs), which corresponds to Wautersia eutropha strain JMS34, deposited under the access number NRRL B-30817 a product for the bioremediation of environments contaminated with PCBs, where the product includes a bacterial inoculum of this recombinant strain and a method for the bioremediation of environments contaminated with PCBs, which uses this product for the bioremediation.

The present invention provides a microorganism and a bacterial agent thereof, and applications of the microorganism and the bacterial agent thereof. The microorganism is Enterbacter hormaechei Y-29, wherein the preservation number of the strain is CCTCC NO:M2016049. The preparation method of the Enterbacter hormaechei Y-29 bacterial agent comprises: activating preserved Enterbacter hormaechei Y-29, culturing a seed liquid, fermenting, carrying out centrifuging separation to obtain the Enterbacter hormaechei Y-29, and mixing the Enterbacter hormaechei Y-29 and a Tween solution to obtain the Enterbacter hormaechei Y-29 bacterial agent, wherein the mixing ratio is that per 60 mL of the Tween solution contains 1 g of the bacteria. According to the present invention, the Enterbacter hormaecheiY-29 and the bacterial agent thereof can provide significant bioremediation effects on petroleum pollution, and can provide excellent degradation effects on petroleum in crude oil-polluted and petroleum-polluted soil; and the Enterbacter hormaechei Y-29 bacterial agent has advantages of short production cycle, low cost, simple use, no secondary pollution, low influence on environment and the like,can enrich the existing petroleum pollution degradation strain resources, and has wide application prospects in petroleum-polluted soil remediation.

The invention discloses bio-fertilizer for efficient degradation of atrazine and a preparation method thereof. Wheat straws, plant ash, saw dust and self-made zymophyte fertilizer beneficial for strain survival and growth are compounded and the compound, Exiguobacterium strain BTAH1 and Shinella granuli HBZA0511 are mixed and are fermented to form the bio-fertilizer. The self-made zymophyte fertilizer is prepared from nutrition bases such as cake, wheat bran, corn straws and fish bone powder, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus rhamnosus through fermentation. The bio-fertilizer is sowed in the field so that soil atrazine herbicide residual quantity is fast reduced by 95% or more, is conducive to soil bioremediation, is suitable for green agricultural product production and improves soil organic content.

The invention relates to the field of microorganisms, in particular to cupriavidus and application of the cupriavidus. The cupriavidus is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is September 25, 2017, and the preservation number is CCTCC M 2017546. Bacterial strain obtained by the invention can degrade 2-chloro-4-nitrophenol by taking phloroglucinol as a ring-opening substrate, and the strain provided by the invention is gram-negative bacteria capable of metabolizing 2-chloro-4-nitrophenol by a 1,2,4-benzenetriol way. On the other hand, the prepared remediation microbial inoculum can completely degrade 100 ppm of 2-chloro-4-nitrophenol in contaminated soil within 12 days. Therefore, the strain has good application prospects in the biological treatment of 2-chloro-4-nitrophenol pollution.

The invention belongs to the technical field of agricultural microbial agent, and especially relates to a preparation method of a microbial insecticide. The preparation method comprises following steps: 1, burkholderia bacteria slant cultivation; 2, burkholderia shake-flask culture; 3, burkholderia seed culture; and 4, burkholderia fermentation culture. The microbial insecticide is used in plant growing and breeding process, can be used for degrading harmful substances accumulated in fields after successive plantation, controlling plant insects and diseases, and promoting plant growth, and possesses biological control and biological repairing functions. The burkholderia bacteria are capable of producing a plurality of metabolites with antibacterial activity such as siderophore, azophenylene, pyrrolnitrin, phenylpyrrole, monoterpenoid alkaloids, Cepaciamide A, Cepacidine A, Cepacin A, and can be applied in fields such as biological control and degrading of toxins.

The invention discloses application of rhodococcus ruber and a preparation thereof in pyrethroid pesticide pollution remediation. The bacterial strain is a gram positive bacterium Rhodococcus ruber bacterial strain Y14, and is preserved in Guangdong Microbial Culture Collection Center on July 21, 2021, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61813. The strain has remarkable pyrethroid insecticide biodegradation capacity, especially has the highest activity on D-cyhalothrin, is prepared into a suspension with the thallus number of not less than 1.0 * 10 < 6 > CFU.mL <-1 >, can completely remove 50 mg.L <-1 > D-cyhalothrin in a water body after being treated for 6 days, and also has an efficient bioremediation effect on polluted soil. The invention provides a new thought for solving the problems of excessive pyrethroid pesticide residue and environmental pollution in agricultural production, enriches the germplasm resource library of pesticide degrading bacteria, and has important practical application value for producing non-toxic and pollution-free green agricultural products.

The invention discloses a method for separating high-cadmium-resistance sulfate reducing bacteria. The method comprises the following steps that 1) a sulfate reducing bacteria sample from a cadmium polluted site is activated; 2) the sulfate reducing bacteria sample is separated and purified; 3) a cadmium-resistance strain is screened; 4) the high-cadmium-resistance bacteria is subjected to oxygen-resistant domestication; 5) testing is conducted on a laboratory scale.

The invention belongs to the technical field of petroleum hydrocarbon polluted soil repairing, and more specifically relates to a method used for repairing petroleum hydrocarbon polluted soil throughcooperation of oxidation with lawn plants. The method comprises following steps: 1, spraying of an aqueous hydrogen peroxide solution is carried out, wherein 300ml/L of the aqueous hydrogen peroxide solution is sprayed onto petroleum hydrocarbon polluted soil; 2, adjusting of pH, wherein a sulfuric acid aqueous solution is sprayed onto the petroleum hydrocarbon polluted soil after spraying of theaqueous hydrogen peroxide solution to adjust the pH value of the petroleum hydrocarbon polluted soil to be 1 to 3; 3, spraying of a FeSO4 aqueous solution, wherein 1mol/L FeSO4 aqueous solution is sprayed onto the petroleum hydrocarbon polluted soil after pH adjusting, and then standing is carried out for 24h or longer; and 4, plantation of lawn plants, wherein lawn plants are planted on the petroleum hydrocarbon polluted soil, the seed sowing density is controlled to be 10 to 20g/m<2>. According to the method, oxidation is combined with lawn plant treatment to improve the repairing effect ofpetroleum hydrocarbon polluted soil.