39results about How to "Promote bioremediation" patented technology

The present invention discloses the formulation and use of advanced solid-media chemical compositions in the preferred forms of pellets, tablets, capsules, or other similar forms which are designed and intended to enhance the removal of a broad range of recalcitrant organic and inorganic contaminants from a variety of difficult-to-treat environments, in particular, sediments beneath water bodies, by providing an improved means of promoting the anaerobic, biologically mediated degradation, transformation, and / or detoxification of the contaminants. Specific properties of the pellet, tablets, capsules, or other similar forms of the compositions are disclosed which enable the variation in the settling velocity of the compositions and hence the depth to which the compositions will penetrate the underlying contaminated sediments. The compositions comprise carbonaceous co-substrates, inorganic and organic anaerobic electron acceptors, organic and inorganic nutrients to promote the growth of contaminant-degrading microorganisms, and inoculum of naturally occurring microorganisms which act to promote the biodegradation of contaminants.

A process for the remediation and conditioning of soil containing a contaminant. The process comprises (a) mixing the soil with solid material comprising a source of microbial nourishment, (b) inoculating the mixed soil with a diverse microbe population capable of digesting the contaminant, and (c) maintaining the moisture content of the inoculated soil at a level sufficient to support microbial growth. The process can include the additional steps of applying a suspension fertilizer to the mixed soil and / or applying an oxygen-releasing compound to the mixed soil. Step (b) can be repeated to sufficiently reduce the level of contamination.

The invention discloses an aquaculture water quality balancing purification agent, which is prepared from the following raw materials by weight: 10-25 parts of tourmaline, 20-40 parts of montmorillonite, 5-10 parts of photosynthetic bacteria, 8-24 parts of actinomycetes, 40-60 parts of zeolite, 10-15 parts of bacillus subtilis, 10-15 parts of Streptococcus faecalis, 10-25 parts of Soybean molasses, 5-10 parts of sodium dihydrogen phosphate, 3-6 parts of tryptophan, 8-16 parts of sodium humate, 10-15 parts of sodium thiosulfate, and 10-20 parts of nitrobacteria. The aquaculture water quality balancing purification agent provided by the invention can reduce harmful substances in water, balance water nutrition, control outbreak of cyanobacteria, activate growth of beneficial algae, increase the dissolved oxygen of water, and improve the content of beneficial microorganisms.

The invention provides a method capable of facilitating bioremediation of oil-contaminated soil. Oxidation remediation is performed firstly and then bioremediation is performed on the contaminated soil. During oxidation remediation, a H2O2 solution serves as an oxidation reagent; after oxidation remediation, the soil is oxidized through the H2O2 solution to generate nutrition so as to facilitate later-stage bioremediation; during bioremediation, no extra nutrition of microorganisms is needed, and bioremediation is performed directly through self microorganisms in the contaminated soil. By means of the method, a large quantity of soluble nutrition can be released by the contaminated soil and supplied to the microorganisms through chemical oxidation, and no extra nutrient substance is needed. Therefore, the remediation cost is reduced, the operation is simple, a few steps are needed, and defects such as secondary contamination are avoided. Under the extra condition of soluble iron, a large quantity of soluble nutrition can be released, and the subsequent bioremediation is further facilitated.

The invention discloses a microbial strain for degrading long-chain alkanes and application of the microbial strain. The microbial strain is acinetobacter junii numbered as C12, and is collected in China General Microbiological Culture Collection Center on May 30, 2019 with the collection number of CGMCC NO. 17873. A biological emulsification effect can be achieved during culture of the microbialstrain, alkane solubilization and emulsification can be promoted, and the alkanes are easily degraded and used as carbon sources or energy by microorganisms. The strain is easy to culture, has high degradation efficiency, and has a potential in wide application to green and efficient remediation of petroleum hydrocarbon contaminated soil and water.

The invention belongs to the field of microorganism and relates to rhizobium capable of degrading polychlorinated biphenyl and an application thereof. Sinorhizobium melilon SL1 is preserved in China General Microbiological Culture Collection Center, CGMCC on December, 10th, 2012. The collection number is CGMCC No.6962. The strain can grow by the utilization of polychlorinated biphenyl as the sole carbon source and energy, and has good capability of degrading both 2,4,4'-TCB and 3,3',4,4'-PCB under the condition of a shake flask in a laboratory. Degradation conditions are optimized, and the polychlorinated biphenyl degrading effect of the strain is further raised. The strain provides foundations for biodegradation of polychlorinated biphenyl and water body pollution restoration.

A method for facilitating growth of microbial communities for in-situ bodies of flowing water for the sake of bio-remediating contaminated water and / or producing useful biomass.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas capable of oxidizing antimony and arsenic and application of the pseudomonas. A pseudomonas strain is separated and screened from antimony-polluted soil in a stannary in Lengshuijiang City, Hunan Province and has efficient antimony and / or efficient arsenic oxidation performance; bacteria can still survive in a solution and soil with high antimony content and / or arsenic content, has relatively good antimony tolerance and arsenic tolerance and has excellent antimony arsenic oxidability and / orarsenic oxidability after being tested, so that an important foundation is laid for development of a technique for removing antimony and / or arsenic through adsorption of the microorganisms. Meanwhile,the strain combined with Fe<3+> has a good improvement effect, the oxidizing efficiency of the strain can be improved, and the strain has good development and application potentialities.

The invention relates to the technical field of management of oil polluted environments, in particular to an oleophylic fertilizer for bioremediation and a method for preparing the oleophylic fertilizer. The oleophylic fertilizer is composed of lecithin, oleic acid, nitrogen-phosphorus solution and alcohols. The preparation method includes: mixing the lecithin with the alcohols proportionally, heating, ultrasonically dissolving, adding the nitrogen-phosphorus solution and oleic acid of a certain quantity, and performing vortex oscillating to form water-in-oil microemulsion, so that the product is obtained and sealed for storage. After the oleophylic fertilizer contacts with oil pollutants, the oil pollutants can form fine oil drops to be aggregated, the oleophylic fertilizer is attached onto the oil drops to provide nitrogen and phosphorus source needed by oil degrading microorganisms, and bioremediation effect can be remarkably improved.

A composition containing cAMP, cGMP, forskolin, adenylate cyclase or guanylate cyclase and microorganisms is provided to facilitate bioremediation, detoxication and to enhance plant growth in media contaminated with petroleum hydrocarbons. Methods to make the composition and apply it to the contaminated media in order to facilitate bioremediation and detoxication of such contaminants are also provided.

A recombinant bacterium capable to completely degrade or mineralize pollutants such as polychlorobiphenyls (PCBs), which corresponds to Cupriavidus necator strain JMS34, deposited under the access number NRRL B-30817 a product for the bioremediation of environments contaminated with PCBs, where the product includes a bacterial inoculum of this recombinant strain and a method for the bioremediation of environments contaminated with PCBs, which uses this product for the bioremediation.

A recombinant bacterium capable to completely degrade or mineralize pollutants such as polychlorobiphenyls (PCBs), which corresponds to Wautersia eutropha strain JMS34, deposited under the access number NRRL B-30817 a product for the bioremediation of environments contaminated with PCBs, where the product includes a bacterial inoculum of this recombinant strain and a method for the bioremediation of environments contaminated with PCBs, which uses this product for the bioremediation.

The present invention provides a microorganism and a bacterial agent thereof, and applications of the microorganism and the bacterial agent thereof. The microorganism is Enterbacter hormaechei Y-29, wherein the preservation number of the strain is CCTCC NO:M2016049. The preparation method of the Enterbacter hormaechei Y-29 bacterial agent comprises: activating preserved Enterbacter hormaechei Y-29, culturing a seed liquid, fermenting, carrying out centrifuging separation to obtain the Enterbacter hormaechei Y-29, and mixing the Enterbacter hormaechei Y-29 and a Tween solution to obtain the Enterbacter hormaechei Y-29 bacterial agent, wherein the mixing ratio is that per 60 mL of the Tween solution contains 1 g of the bacteria. According to the present invention, the Enterbacter hormaecheiY-29 and the bacterial agent thereof can provide significant bioremediation effects on petroleum pollution, and can provide excellent degradation effects on petroleum in crude oil-polluted and petroleum-polluted soil; and the Enterbacter hormaechei Y-29 bacterial agent has advantages of short production cycle, low cost, simple use, no secondary pollution, low influence on environment and the like,can enrich the existing petroleum pollution degradation strain resources, and has wide application prospects in petroleum-polluted soil remediation.

The invention discloses bio-fertilizer for efficient degradation of atrazine and a preparation method thereof. Wheat straws, plant ash, saw dust and self-made zymophyte fertilizer beneficial for strain survival and growth are compounded and the compound, Exiguobacterium strain BTAH1 and Shinella granuli HBZA0511 are mixed and are fermented to form the bio-fertilizer. The self-made zymophyte fertilizer is prepared from nutrition bases such as cake, wheat bran, corn straws and fish bone powder, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus rhamnosus through fermentation. The bio-fertilizer is sowed in the field so that soil atrazine herbicide residual quantity is fast reduced by 95% or more, is conducive to soil bioremediation, is suitable for green agricultural product production and improves soil organic content.

The invention discloses a marine nitrobacterium, a preparation method therefor and application of the marine nitrobacterium. Through treating leather wastewater with sulfitobacter dubius PT04, which has high saline matter tolerance and can remove hexavalent chrome and ammonia nitrogen from a contaminated water source under aerobic conditions, the problem that the hexavalent chrome and ammonia nitrogen removing capability of the existing nitrobacteria is lowered due to inhibition of toxic substances in the leather wastewater is solved; and through experiments, it is proven that the sulfitobacter dubius PT04 has relatively high biological safety, the Cr(VI) and the ammonia nitrogen are converted into products with lower toxicity by using an enzymatic reaction, and the marine nitrobacterium shows a good effect in treatment on the ammonia nitrogen and hexavalent chrome in the leather wastewater, has the advantages of good reduction effect, high nitrifying efficiency and environment-friendliness and has a good application prospect in the field of bioremediation.

The invention relates to the field of microorganisms, in particular to cupriavidus and application of the cupriavidus. The cupriavidus is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is September 25, 2017, and the preservation number is CCTCC M 2017546. Bacterial strain obtained by the invention can degrade 2-chloro-4-nitrophenol by taking phloroglucinol as a ring-opening substrate, and the strain provided by the invention is gram-negative bacteria capable of metabolizing 2-chloro-4-nitrophenol by a 1,2,4-benzenetriol way. On the other hand, the prepared remediation microbial inoculum can completely degrade 100 ppm of 2-chloro-4-nitrophenol in contaminated soil within 12 days. Therefore, the strain has good application prospects in the biological treatment of 2-chloro-4-nitrophenol pollution.

The present invention provides a biological type bone repairing material and a preparation method thereof, and particularly discloses a heterogeneous decellularized bone repairing material and a preparation method and an application thereof. The preparation method comprises the following steps: (1) cancellous bones of mammals are taken and crushed into granules, the cancellous bone granules are rinsed until no visible bone marrow exists, and the rinsed cancellous bone granules are dried; (2) the obtained bone repairing material in the step (1) is placed in hydrogen peroxide to be soaked, and rinsing and drying are conducted; (3) the obtained bone repairing material in the step (2) is placed in a sodium hydroxide solution to be soaked, and rinsing and drying are conducted; and (4) the obtained bone repairing material in the step (3) is placed in a mixed solution of ethanol and acetone to be soaked, and rinsing and drying are conducted to obtain the heterogeneous decellularized bone repairing material. Compressive strength and elasticity modulus of the prepared heterogeneous decellularized bone repairing material by the technology are consistent with compressive strength and elasticity modulus of human body cancellous bones. The biological type bone repairing material is very suitable for repairing human body bone defects.

The invention belongs to the technical field of agricultural microbial agent, and especially relates to a preparation method of a microbial insecticide. The preparation method comprises following steps: 1, burkholderia bacteria slant cultivation; 2, burkholderia shake-flask culture; 3, burkholderia seed culture; and 4, burkholderia fermentation culture. The microbial insecticide is used in plant growing and breeding process, can be used for degrading harmful substances accumulated in fields after successive plantation, controlling plant insects and diseases, and promoting plant growth, and possesses biological control and biological repairing functions. The burkholderia bacteria are capable of producing a plurality of metabolites with antibacterial activity such as siderophore, azophenylene, pyrrolnitrin, phenylpyrrole, monoterpenoid alkaloids, Cepaciamide A, Cepacidine A, Cepacin A, and can be applied in fields such as biological control and degrading of toxins.

The invention discloses application of rhodococcus ruber and a preparation thereof in pyrethroid pesticide pollution remediation. The bacterial strain is a gram positive bacterium Rhodococcus ruber bacterial strain Y14, and is preserved in Guangdong Microbial Culture Collection Center on July 21, 2021, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61813. The strain has remarkable pyrethroid insecticide biodegradation capacity, especially has the highest activity on D-cyhalothrin, is prepared into a suspension with the thallus number of not less than 1.0 * 10 < 6 > CFU.mL <-1 >, can completely remove 50 mg.L <-1 > D-cyhalothrin in a water body after being treated for 6 days, and also has an efficient bioremediation effect on polluted soil. The invention provides a new thought for solving the problems of excessive pyrethroid pesticide residue and environmental pollution in agricultural production, enriches the germplasm resource library of pesticide degrading bacteria, and has important practical application value for producing non-toxic and pollution-free green agricultural products.

The invention discloses a method for separating high-cadmium-resistance sulfate reducing bacteria. The method comprises the following steps that 1) a sulfate reducing bacteria sample from a cadmium polluted site is activated; 2) the sulfate reducing bacteria sample is separated and purified; 3) a cadmium-resistance strain is screened; 4) the high-cadmium-resistance bacteria is subjected to oxygen-resistant domestication; 5) testing is conducted on a laboratory scale.

The invention discloses an aquaculture water purifying agent. The aquaculture water purifying agent comprises the following components in part by weight: 5 to 20 parts of tourmaline, 20 to 60 parts of montmorillonite, 100 to 500 parts of zeolite, 5 to 30 parts of molasses, 0.5 to 5 parts of phosphate, 0.1 to 1 part of tryptophan and 10 to 50 parts of microbial agent. The purifying agent is compounded by using the microbial agent, mineral biological activating agents and nutrient elements; and the tourmaline, the montmorillonite and the zeolite can promote microbe growth and metabolic activity, and can be attached to or fix microbes to play a barrier protection role. The nutrient elements can regulate the carbon nitrogen ratio balance of the water and promote the growth and the degrading effect of the microbes, and the zeolite and the montmorillonite are good controlled release carriers and have remarkable controlled release effect on the nutrient elements. The purifying agent can strengthen and activate the metabolic activity and the environmental adaptability of the microbial agent; and by compounding the minerals and the nutrient elements, the optimal ecological environment of microbe degradation is regulated and controlled, a biodegradable micro-ecological system for constructing the culture water is optimized and biological repair of the water is promoted.

The invention belongs to the technical field of petroleum hydrocarbon polluted soil repairing, and more specifically relates to a method used for repairing petroleum hydrocarbon polluted soil throughcooperation of oxidation with lawn plants. The method comprises following steps: 1, spraying of an aqueous hydrogen peroxide solution is carried out, wherein 300ml / L of the aqueous hydrogen peroxide solution is sprayed onto petroleum hydrocarbon polluted soil; 2, adjusting of pH, wherein a sulfuric acid aqueous solution is sprayed onto the petroleum hydrocarbon polluted soil after spraying of theaqueous hydrogen peroxide solution to adjust the pH value of the petroleum hydrocarbon polluted soil to be 1 to 3; 3, spraying of a FeSO4 aqueous solution, wherein 1mol / L FeSO4 aqueous solution is sprayed onto the petroleum hydrocarbon polluted soil after pH adjusting, and then standing is carried out for 24h or longer; and 4, plantation of lawn plants, wherein lawn plants are planted on the petroleum hydrocarbon polluted soil, the seed sowing density is controlled to be 10 to 20g / m<2>. According to the method, oxidation is combined with lawn plant treatment to improve the repairing effect ofpetroleum hydrocarbon polluted soil.

The present invention relates to the technical field of agricultural microbial bacterial agents, in particular to a preparation method of microbial insecticides. The specific steps are as follows: Step 1: Burkholderia bacterial slant culture; Step 2: Burkholderia shaking Bottle cultivation; Step 3: Burkholderia bacterium seed cultivation; Step 4: Burkholderia bacterium fermentation and cultivation, microbial insecticide of the present invention is attached to plant body growth and perfect growth process; can Decompose the harmful substances accumulated in the continuous planting field for the prevention and control of plant pests, and have the functions of biological control, plant growth promotion and bioremediation. Bacteria of the genus Burkholderia can produce a variety of metabolites with antibacterial activity, such as siderophore, phenazine, nipyrrolicin, phenylpyrrole, monoterpene alkaloid, Cepacamide A, Cepacidine A, Cepacin A, etc. It has been used in biological control, decomposition of toxic substances and other fields.

The invention discloses a marine nitrifying bacterium and its preparation method and application, by providing a suspicious sulfite bacterium (Sulfitobacter dubius) that has high tolerance to salt and can remove hexavalent chromium and ammonia nitrogen in polluted water sources under aerobic conditions PT04 treats leather wastewater, which solves the problem that the existing nitrifying bacteria have reduced ability to remove hexavalent chromium and ammonia nitrogen due to the inhibition of toxic substances in leather wastewater, and it has been proved by experiments that the suspected sulfite bacteria PT04 itself has a high biological Safety, using enzymatic reaction to convert Cr(VI) and ammonia nitrogen into less toxic products, showing good results in the treatment of ammonia nitrogen and hexavalent chromium in leather wastewater, with good reduction effect, high nitrification efficiency, and environmental protection It has good application prospects in the field of bioremediation.

The invention discloses the application of Rhodococcus rhodochrous and its preparation in the remediation of pyrethroid pesticide pollution. The strain is the gram-positive bacterium Rhodococcus ruber strain Y14, and was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 21, 2021, and the deposit number is GDMCC NO: 61813. The strain has significant biodegradation ability of pyrethroid insecticides, especially the highest activity to dextrofenothrin, and the number of bacterial cells is not less than 1.0×10. 6 CFU·mL ‑1 The suspension of 50mg·L in water after 6 days of treatment ‑1 D-Cyphenothrin is completely removed, and it also has a high-efficiency bioremediation effect on polluted soil. It provides a new idea to solve the problems of excessive pyrethroid pesticide residues and environmental pollution in agricultural production, and also enriches the germplasm resource bank of pesticide-degrading bacteria, which is of great practical importance for the production of non-toxic and pollution-free green agricultural products. Value.

The invention discloses a multi-element composite microorganism regulating agent for regulating and controlling an aquatic water environment. The multi-element composite microorganism regulating agent mainly consists of the following components in parts by weight: 10-100 parts of a composite mineral carrier, 5-40 parts of a microbial agent, 0.5-1 part of liquorice, 0.5-2 parts of charred hawthorn powder, 1-6 parts of biomass char, 0.1-5 parts of carboxymethylcellulose, 3-8 parts of potassium fulvic acid, 0.5-0.35 part of pyridoxine, 0.2-1 part of paclobutrazol, 2-3.6 parts of polyvinyl acetate, 3-20 parts of expanded vermiculite and 5-20 parts of fried wheat bran. By adopting the multi-element composite microorganism regulating agent, microbial communities of a pool can be directionally adjusted and controlled, beneficial microorganisms can be increased, biological population diversity can be maintained, beneficial organisms can be in a dominant position, a biological degradation microorganism system of an aquatic water body can be optimized and established, bioremediation of the aquatic water body can be promoted, multiple nutrient elements can be provided for biont in the water body, growth and propagation of the biont can be promoted, and in addition, harmful substances in the water body can be adsorbed, so that stabilized regulation and control on the aquatic water body and effective prevention of diseases can be achieved, cultivation pollution can be reduced, and the quality and the security of an aquaculture product can be improved.

The invention belongs to the field of microorganism and relates to rhizobium capable of degrading polychlorinated biphenyl and an application thereof. Sinorhizobium melilon SL1 is preserved in China General Microbiological Culture Collection Center, CGMCC on December, 10th, 2012. The collection number is CGMCC No.6962. The strain can grow by the utilization of polychlorinated biphenyl as the sole carbon source and energy, and has good capability of degrading both 2,4,4'-TCB and 3,3',4,4'-PCB under the condition of a shake flask in a laboratory. Degradation conditions are optimized, and the polychlorinated biphenyl degrading effect of the strain is further raised. The strain provides foundations for biodegradation of polychlorinated biphenyl and water body pollution restoration.

The invention relates to the technical field of management of oil polluted environments, in particular to an oleophylic fertilizer for bioremediation and a method for preparing the oleophylic fertilizer. The oleophylic fertilizer is composed of lecithin, oleic acid, nitrogen-phosphorus solution and alcohols. The preparation method includes: mixing the lecithin with the alcohols proportionally, heating, ultrasonically dissolving, adding the nitrogen-phosphorus solution and oleic acid of a certain quantity, and performing vortex oscillating to form water-in-oil microemulsion, so that the product is obtained and sealed for storage. After the oleophylic fertilizer contacts with oil pollutants, the oil pollutants can form fine oil drops to be aggregated, the oleophylic fertilizer is attached onto the oil drops to provide nitrogen and phosphorus source needed by oil degrading microorganisms, and bioremediation effect can be remarkably improved.

The invention discloses a nutritional anti-stress bioremediation composition for pigs and a preparation method thereof. The composition comprises the following components: water-soluble Vitamin A powder, water-soluble Vitamin D3, water-soluble Vitamin E, water-soluble Vitamin K3, Vitamin B1, Vitamin B2, Vitamin B6, Vitamin B12, biotin, folic acid, nicotinic acid amine, calcium pantothenate, Vitamin C, carnitine, Beta-carotene, lysine, methionine, threonine, tryptophane, arginine, fructooligosccharide, glutamine, lipoic acid and anhydrous dextrose. The invention also relates to the preparation method of the composition, which comprises the step that after all the components are uniformly mixed, the final product (the nutritional anti-stress bioremediation composition for pigs) can be obtained. According to the invention, the oxidation prevention method is adopted to inhibit the incidence and development processes of stress of pigs, and the bioremediation technology is adopted to repair oxidative damages. The composition has superior effects and stable properties, does not contain any antibiotic additive, can reduce loss caused by stress syndrome and has a better application prospect.