Microbial strain for degrading long-chain alkanes and application of microbial strain

A technology of long-chain alkanes and microbial bacteria, which is applied in the direction of microorganisms, microorganisms, and isolated microorganisms. It can solve the problems of application limitations, inconsistent application conditions of different strains, and poor stability of bacterial flora, so as to achieve convenient application and benefit governance and bioremediation. , the effect of promoting degradation

Pending Publication Date: 2020-02-28
HUATIAN ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was previously reported that a variety of microorganisms can be used to degrade different alkanes. However, in practical applications, there are problems such as inconsistent application conditions of different strains and poor stability of the bacterial flora, which limits the application. Therefore, it is of great significance to screen an efficient alkane-degrading bacteria.

Method used

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  • Microbial strain for degrading long-chain alkanes and application of microbial strain
  • Microbial strain for degrading long-chain alkanes and application of microbial strain
  • Microbial strain for degrading long-chain alkanes and application of microbial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Acquisition, screening and identification of strain Acinetobacter juniiC12.

[0055] Step 1. Sample collection and enrichment culture

[0056] Retrieve soil samples contaminated by crude oil from Dongying Shengli Oilfield Oil Production Plant, weigh 10g of the collected soil samples and add them to 100mL inorganic salt medium, shake well, add 0.1% crude oil to it, and place it at a constant temperature of 30°C and 150rpm Cultivate in a shaking incubator for 7 days;

[0057] Take 1 mL of the culture solution and add it to the inorganic salt medium containing 0.1% n-hexadecane, and repeat the above operation to obtain the enriched culture solution.

[0058] Step two, separation and purification

[0059] Take 1mL of the culture solution and dilute it to 10% by volume with sterile water -1 ,10 -2 ,10 -3 ,10 -4 ,10 -5 ,10 -6 ,10 -7 ,10 -8 Take 100 μL diluted bacterial suspension with concentration gradient and evenly spread it on a solid LB plate, and place it upsi...

Embodiment 2

[0067] Effect of temperature on degradation of n-hexadecane by strain Acinetobacter juniiC12.

[0068] The strain Acinetobacter juniiC12 was inoculated into inorganic salt medium containing 0.1% volume ratio, and cultured in a constant temperature shaker with a rotation speed of 150 rpm and a culture temperature of 15°C, 20°C, 30°C, 35°C, and 40°C for 48 hours. Gas chromatography is used to detect the impact of temperature on the degradation of n-hexadecane by bacterial strains, the results are shown in Figure 4 , it can be seen from the results that the suitable temperature range of the strain is 25 ℃ ~ 40 ℃, which has a wide range of temperature utilization.

[0069] This example shows that the strain Acinetobacter junii C12 has a good degradation effect on n-hexadecane in different temperature environments.

Embodiment 3

[0071] Effect of pH on the degradation of n-hexadecane by strain Acinetobacter junii C12.

[0072] C12 was inoculated into the inorganic salt medium containing n-hexadecane with a volume ratio of 0.1%, and the pH of the medium was adjusted to 3, 5, 7, 9, and 11 respectively, and the temperature was 30°C at a constant temperature shaker with a rotation speed of 150rpm Cultivated in medium for 48h, adopt gas chromatography to detect the influence of pH on bacterial strain Acinetobacter junii C12 degrading n-hexadecane, the results are shown in Figure 5 .

[0073] It shows that the strains have a good degradation rate of n-hexadecane at a pH value of 6-8, and the degradation effect is the best at a pH value of 7, which can be widely used in this range.

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Abstract

The invention discloses a microbial strain for degrading long-chain alkanes and application of the microbial strain. The microbial strain is acinetobacter junii numbered as C12, and is collected in China General Microbiological Culture Collection Center on May 30, 2019 with the collection number of CGMCC NO. 17873. A biological emulsification effect can be achieved during culture of the microbialstrain, alkane solubilization and emulsification can be promoted, and the alkanes are easily degraded and used as carbon sources or energy by microorganisms. The strain is easy to culture, has high degradation efficiency, and has a potential in wide application to green and efficient remediation of petroleum hydrocarbon contaminated soil and water.

Description

technical field [0001] The invention relates to a microbial bacterium for degrading long-chain alkanes and its application. Background technique [0002] Oil is an important strategic energy source, known as "black gold". The petroleum industry is the main lifeline of the national economy and an important manifestation of the country's comprehensive national strength. The development of the petroleum industry has been widely valued by countries all over the world. With the development of the petroleum industry, the problem of petroleum pollution has become increasingly prominent. Crude oil leakage and oil spill accidents in the process of petroleum exploration, processing, refining, transportation and use have seriously polluted water bodies and soil. The waste produced in the process of oil mining and smelting is piled up around factories and mines, and the content of petroleum hydrocarbons in the surrounding area increases after being washed by rainwater. In severe cases,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C12R1/01C02F101/32
CPCC12N1/02C02F3/344B09C1/10C02F2101/32C12R2001/01C12N1/205
Inventor 崔倩倩程寒飞孟溪
Owner HUATIAN ENG & TECH CORP MCC
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