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Rhizobium capable of degrading polychlorinated biphenyl and application thereof

A technology of polychlorinated biphenyls and trichlorobiphenyls, applied in the field of microorganisms

Active Publication Date: 2013-11-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the resources of rhizobia that can degrade PCBs in my country.

Method used

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  • Rhizobium capable of degrading polychlorinated biphenyl and application thereof
  • Rhizobium capable of degrading polychlorinated biphenyl and application thereof
  • Rhizobium capable of degrading polychlorinated biphenyl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening and identification of polychlorinated biphenyl degrading rhizobia

[0023] Medium preparation:

[0024] Modified basal salt medium (degradation medium): KH 2 PO 4 3.7g, K 2 HPO 4 ·3H 2 O5.2g, NH 4 Cl1.0g, Na 2 SO 4 1.0g, MgSO 4 ·7H 2 O0.2g, trace metal salt solution 1mL, add water to 1000mL, pH7.0~7.5. Add the acetone solution of PCB28 (2,4,4'-TCB) to make the concentration reach 1mg / L, as the only carbon source, and expose it until the acetone volatilizes for use. Add 18g / L agar to the solid medium.

[0025] Trace metal salt solution (1000mL deionized water): FeCl 2 4H 2 O0.3g, CoCl 2 ·6H 2 O0.038g, MnCl 2 4H 2 O0.02g, ZnCl 2 0.014g, H 3 BO 3 0.0124g, Na 2 MoO 4 2H 2 O0.04g, CuCl 2 2H 2 O0.0034g.

[0026] LB medium: peptone 10g, yeast powder 5g, NaCl 10g, distilled water to 1000mL. Add 18g / L agar to the solid medium.

[0027] Domestication and screening of degrading rhizobia:

[0028] It was isolated from the rhizosphere...

Embodiment 2

[0033] Example 2 SLI rhizobia to different concentrations of 2,4,4'-TCB and 3,3',4,4'-PCB degradation rate

[0034] Medium required:

[0035]Mannitol yeast juice agar medium (YMA) medium: mannitol 10.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.5g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.2g, sodium chloride (NaCl) 0.1g, yeast juice (1%) 100mL, calcium carbonate (CaCO 3 ) 3.0g, distilled water 900mL, pH 7.0~7.2.

[0036] Inorganic salt basal medium, the mass ratio of the medium consists of: KH 2 PO 4 3.7g, K 2 HPO 4 ·3H 2 O5.2g, NH 4 Cl1.0g, Na 2 SO 4 1.0g, MgSO 4 ·7H 2 O0.2g, trace metal salt solution 1mL, add water to 1000mL.

[0037] Trace metal salt solution (1000mL deionized water): FeCl 2 4H 2 O0.3g, CoCl 2 ·6H 2 O0.038g, MnCl 2 4H 2 O0.02g, ZnCl 2 0.014g, H 3 BO 3 0.0124g, Na 2 MoO 4 2H 2 O0.04g, CuCl 2 2H 2 O0.0034g.

[0038] 2.1 Fermentation culture of rhizobia

[0039] Inoculate the SL1 rhizobia strains on a YMA solid plate at 30°C for...

Embodiment 3

[0050] The influence of embodiment 3 different fermentation conditions on the degradation rate of SL1 bacterial strain

[0051] Orthogonal experiments were carried out on the four factors of different temperature, pH, inoculum volume and air flow to explore the influence of the degradation rate of the strain.

[0052] During the fermentation process of the SL1 strain, pH 5, 6, 7, 8, 9 were selected; temperature 20, 25, 30, 35, 40°C; , 100, and 150mL four factors were used for orthogonal experiments, and the levels of the factors are shown in Table 3. After shaken culture in liquid shake flask, the cells were collected by centrifugation and transferred to the basic inorganic salt medium containing 10mg / L2,4,4′-TCB, 30°C, 180r min -1 Culture 7d. The results are shown in Table 4 below, the best combination obtained in this experiment is A 2 B 3 C 3 D. 4 , that is, the temperature is 25°C, the pH is 7, the inoculum volume is 3mL, and the ventilation volume is 100mL. The inf...

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Abstract

The invention belongs to the field of microorganism and relates to rhizobium capable of degrading polychlorinated biphenyl and an application thereof. Sinorhizobium melilon SL1 is preserved in China General Microbiological Culture Collection Center, CGMCC on December, 10th, 2012. The collection number is CGMCC No.6962. The strain can grow by the utilization of polychlorinated biphenyl as the sole carbon source and energy, and has good capability of degrading both 2,4,4'-TCB and 3,3',4,4'-PCB under the condition of a shake flask in a laboratory. Degradation conditions are optimized, and the polychlorinated biphenyl degrading effect of the strain is further raised. The strain provides foundations for biodegradation of polychlorinated biphenyl and water body pollution restoration.

Description

technical field [0001] In the field of microorganisms, the invention relates to a rhizobium strain capable of degrading polychlorinated biphenyls and its application. Background technique [0002] Polychlorinated biphenyls (PCBs) are a class of artificially synthesized organic compounds with strong chemical inertness and biorefractory biodegradability. Soil is the main destination for them in the environment. Because many PCBs can accumulate in organisms and have the "three causes" effects of carcinogenicity, teratogenicity, and mutagenicity, they have been listed as one of the first 12 persistent organic pollutants (POPs) that need to be reduced and controlled. How to remediate PCBs-contaminated soil has become a hot spot of concern. [0003] At present, safe and economical bioremediation, especially microbial remediation measures, has become a very promising PCBs contamination remediation technology. Since Ahmed and Focht screened out two strains of Achromobacter sp. tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 徐莉李方卉胡锋张腾昊张振陈雄李辉信焦加国刘满强陈小云
Owner NANJING AGRICULTURAL UNIVERSITY
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