A kind of preparation method of microbial insecticide

A microbial pesticide and seed technology, applied in the field of microbial pesticide preparation, can solve problems such as single application range, strong antagonism, and environmental restrictions, and achieve the effect of increasing growth rate and promoting plant growth

Active Publication Date: 2018-03-27
上海乾界生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its growth and reproduction are largely restricted by external conditions, and it is restricted by the environment in practical applications.
3. Viral insecticides: The infection routes of insect viruses mainly include ingestion infection and skin infection. At present, this type of preparation is in the research and development stage, and industrial production is not yet mature.
[0004] In summary, the existing microbial insecticides have a relatively single application range in the actual application process, strong antagonism between strains, and a narrow insecticidal spectrum; existing products in terms of industrial application, environmental adaptability, and stress resistance There are also many deficiencies, which limit the wide application of microbial agents on crop insecticides.

Method used

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  • A kind of preparation method of microbial insecticide
  • A kind of preparation method of microbial insecticide
  • A kind of preparation method of microbial insecticide

Examples

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preparation example Construction

[0046]The preparation method of the Acorus calamus extract described in the examples is as follows: crush the Calamus calamus and pass through a 16-mesh sieve to obtain a calamus calamus powder; mix the calamus calamus powder and water with a solid-to-liquid mass ratio of 1:20; , Ultrasonic extraction at 60°C for 2 hours at an ultrasonic frequency of 40kHz, centrifuged at a speed of 5000 rpm for 20min, and the upper clear layer was concentrated under reduced pressure at a vacuum of 0.06MPa and a temperature of 70°C to a density of 1.05 g / mL (25°C) to obtain the Acorus calamus extract. Acorus tatarinowii, Latin scientific name: Acorus tatarinowii Schott, is the rhizome of Araceae plant Acorus tatarinowii. Specifically, the calamus calamus provided by Anguo Yuejie Chinese Medicinal Materials Co., Ltd. was used.

[0047] The preparation method of the mulberry leaf extract described in the examples is as follows: crush the mulberry leaves and pass through a 16-mesh sieve to obtai...

Embodiment 1

[0053] Step 1: Burkholderia bacteria slant culture

[0054] Bacteria of the genus Burkholderia were inoculated on the slant medium for slant culture, the inoculation mass percentage was 5%, the temperature of the slant culture was 28°C, and the time was 24h, the mass of each component in the slant medium The percentages are: sodium chloride 0.5%, peptone 1%, agar powder 1.8%, the rest is water, and the pH value is adjusted to 7.0;

[0055] Step 2: Shake Flask Culture of Burkholderia Bacteria

[0056] The culture solution obtained in step 1 was inoculated into the shake flask medium for shake flask culture, the inoculation mass percentage was 10%, the shake flask culture temperature was 28°C, the time was 24h, and the rotation speed was 220rpm. In the shake flask culture medium The mass percentage of each component is: 0.2% of dipotassium hydrogen phosphate, 0.5% of beef extract, 1% of peptone, and the rest is water, and the pH value is adjusted to 7.0;

[0057] Step 3: Burkh...

Embodiment 2

[0064] It is basically the same as Embodiment 1, the difference is only in:

[0065] The mass percent of each component in the fermentation medium described in the step 4 is: beef extract 0.5%, peptone 1%, Shichangpu extract 0.8%, defoamer 0.005%, and the rest is water, and the pH value is adjusted to 7.0.

[0066] In this example, after 28 hours of fermentation in step 4, the number of bacteria in the fermentation broth reached 5×10 8 cfu / ml. It can be seen that adding the Acorus calamus extract to the fermentation medium will accelerate the fermentation rate of the bacteria of the genus Burkholderia, and within the same fermentation time, the productivity of the bacteria of the genus Burkholderia is higher.

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Abstract

The present invention relates to the technical field of agricultural microbial bacterial agents, in particular to a preparation method of microbial insecticides. The specific steps are as follows: Step 1: Burkholderia bacterial slant culture; Step 2: Burkholderia shaking Bottle cultivation; Step 3: Burkholderia bacterium seed cultivation; Step 4: Burkholderia bacterium fermentation and cultivation, microbial insecticide of the present invention is attached to plant body growth and perfect growth process; can Decompose the harmful substances accumulated in the continuous planting field for the prevention and control of plant pests, and have the functions of biological control, plant growth promotion and bioremediation. Bacteria of the genus Burkholderia can produce a variety of metabolites with antibacterial activity, such as siderophore, phenazine, nipyrrolicin, phenylpyrrole, monoterpene alkaloid, Cepacamide A, Cepacidine A, Cepacin A, etc. It has been used in biological control, decomposition of toxic substances and other fields.

Description

technical field [0001] The invention relates to the technical field of agricultural microbial bacterial agents, in particular to a preparation method of microbial pesticides. Background technique [0002] Plant diseases and insect pests are the great enemies of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the annual average loss of production reduction caused by plant diseases is 15% to 20% of the total output. For a long time, the preparations used to prevent or exterminate farmland pests are generally chemical pesticides, such as 666 and dichlorvos. Long-term and frequent use of chemical pesticides has led to the emergence of pest resistance and gradually reduced the effectiveness of chemical pesticides. At present, the main solution is to increase the dosage of pesticides, which will directly lead to the increase of agricultural costs; the resistance of pests to pesticides will increase, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N43/16A01P7/00C12R1/01
CPCA01N43/16A01N63/00C12N1/20C12P1/04C12N1/205C12R2001/01A01N2300/00
Inventor 林江一
Owner 上海乾界生物科技有限公司
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